Phylogentic Position, Pigment Content and Optimal Growth Condition of the Unicellular Hydrogen-Producing Cyanobacterial Strains from Korean Coasts

한국 연안산 단세포성 수소생산 남세균 종주들의 분류계통, 색소함량 및 최적성장 환경

  • PARK, JONG-WOO (Fisheries Resources and Environment Division, Southwest Sea Fisheries Research Institute) ;
  • KIM, JU HEE (National Marine Biodiversity Institute of Korea) ;
  • CHO, AE-RA (Fisheries Resources and Environment Division, Southwest Sea Fisheries Research Institute) ;
  • JUNG, YUN-DUK (Fisheries Resources and Environment Division, Southwest Sea Fisheries Research Institute) ;
  • KIM, PYOUNG JOONG (Fisheries Resources and Environment Division, Southwest Sea Fisheries Research Institute) ;
  • KIM, HYUNG-SEOP (Department of Marine Biotechnology, Kunsan National University) ;
  • YIH, WONHO (Department of Marine Biotechnology, Kunsan National University)
  • 박종우 (국립수산과학원 남서해수산연구소 자원환경과) ;
  • 김주희 (국립해양생물자원관) ;
  • 조애라 (국립수산과학원 남서해수산연구소 자원환경과) ;
  • 정연덕 (국립수산과학원 남서해수산연구소 자원환경과) ;
  • 김평중 (국립수산과학원 남서해수산연구소 자원환경과) ;
  • 김형섭 (군산대학교 해양생물공학과) ;
  • 이원호 (군산대학교 해양생물공학과)
  • Received : 2015.04.22
  • Accepted : 2015.07.22
  • Published : 2015.08.31


To set up unicellular cyanobacterial strains with photo-biological $H_2$ production potential, live samples were repeatedly collected from 68 stations in the coastal zone of Korea for the four years since 2005. Among 77 cyanobacterial strains established six (KNU strains, CB-MAL002, 026, 031, 054, 055 and 058) were finally chosen as the excellent strains for $H_2$ production with $H_2$ accumulation over 0.15 mL $H_2\;mL^{-1}$ under general basic $H_2$ production conditions as well as positive $H_2$ production for more than 60 hr. To explore optimum procedures for higher $H_2$ production efficiency of the six cyanobacterial strains, the inter-strain differences in the growth rate under the gradients of water temperature and salinity were investigated. The maximum daily growth rates of the six strains ranged from 1.78 to 2.08, and all of them exhibited $N_2-fixation$ ability. Based on the similarity of the 16S rRNA sequences, all the test strains were quite close to Cyanothece sp. ATCC51142 (99%). The six strains, however, were grouped into separate clades from strain ATCC51142 in the molecular phylogeny diagram. Chlorophyll- a content was 3.4~7.8% of the total dried weight, and the phycoerythrin and phycocyanin contents were half of those in the Atlantic strain, Synechococcus sp. Miami BG03511. The growth of the six strains was significantly suppressed at temperatures above the optimal range, $30{\sim}35^{\circ}C$, to be nearly stopped at $40^{\circ}C$. The growth was not inhibited by high salinities of 30 psu salinity in all the strains while strain CB055 maintained its high growth rate at low salinities down to 15 psu. The euryhaline strains like CB055 might support massive biotechnological cultivation systems using natural basal seawater in temperate latitudes. base seawater. The biological and ecophysiological characteristics of the test strains may contribute to designing the optimal procedures for photo-biological $H_2$ production by unicellular cyanobacteria.


Supported by : 국립수산과학원


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