Effects of Chelidonium Majus Extract on Apoptosis Induction of MDA-MB-231 Human Breast Cancer Cells

백굴채 추출물이 MDA-MB-231 유방암 세포주에서 세포사멸에 미치는 효과

  • Received : 2015.04.21
  • Accepted : 2015.05.06
  • Published : 2015.05.29


Objectives : In this study, we investigated the anti-proliferative and apoptosis inducing effect of water extract of Chelidonium majus (CM) on human breast cancer cell MDA-MB-231. Methods : The MTT assay was used to assess cell proliferation. The expression of apoptosis related gene was assessed by quantitative Real-time PCR. Cell apoptosis detected by flow cytometry using Annexin-V/PI staining. Results : Our data revealed that CM inhibited the cell growth in a dose dependent manner (0, 62.5, 125, 250, 500 μg/ml). CM increased mRNA expression of pro-apoptotic genes Bax, caspase-3, and caspase-9. Annexin-V/PI staining assays revealed that apoptosis-induced cell death increased in a dose-dependent manner in cells. Conclusions : CM induces cell death in MDA-MB-231 human breast cancer cell and shows potentials for use in cancer therapy as apoptosis-inducing agent.


Chelidonium majus;breast cancer;MDA-MB-231;apoptosis;anti-proliferative


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