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Effects of Chelidonium Majus Extract on Apoptosis Induction of MDA-MB-231 Human Breast Cancer Cells

백굴채 추출물이 MDA-MB-231 유방암 세포주에서 세포사멸에 미치는 효과

  • Received : 2015.04.21
  • Accepted : 2015.05.06
  • Published : 2015.05.29

Abstract

Objectives : In this study, we investigated the anti-proliferative and apoptosis inducing effect of water extract of Chelidonium majus (CM) on human breast cancer cell MDA-MB-231. Methods : The MTT assay was used to assess cell proliferation. The expression of apoptosis related gene was assessed by quantitative Real-time PCR. Cell apoptosis detected by flow cytometry using Annexin-V/PI staining. Results : Our data revealed that CM inhibited the cell growth in a dose dependent manner (0, 62.5, 125, 250, 500 μg/ml). CM increased mRNA expression of pro-apoptotic genes Bax, caspase-3, and caspase-9. Annexin-V/PI staining assays revealed that apoptosis-induced cell death increased in a dose-dependent manner in cells. Conclusions : CM induces cell death in MDA-MB-231 human breast cancer cell and shows potentials for use in cancer therapy as apoptosis-inducing agent.

Keywords

Chelidonium majus;breast cancer;MDA-MB-231;apoptosis;anti-proliferative

References

  1. Yoon JH. 2014 breast cancer white paper. Seoul:Korean breast cancer society. 2014:2-5.
  2. International Agency for Research on Cancer. GLOBOCAN 2012: Estimated cancer incidence, mortality and prevalence worldwide in 2012. Lyon. 2014. Available from:URL:http://globocan.iarc.fr/Pages/fact_sheets_cancer.
  3. Korea central cancer registry. Ministry of Health&wealfare. Annual report of cancer statistics in Korea in 2011. 2013. Available from:URL:http://meta.narastat.kr/metasvc/index.do?confmNo=11744&inputYear=2011. Accessed September 1st, 2014.
  4. 陳自明. 婦人良方大全. 臺北:集文書局. 1950:71-5.
  5. Cho JK. The clinical oncology in oriental medicine. Korea:Joomin publisher. 2005:4-5, 533-49.
  6. Korean obstetrics & gynecology. Oriental obstetrics & gynecology(下). Seoul: Euiseongdang. 2012:399.
  7. Gilca M, et al. Chelidonium majus-an integrative review: traditional knowledge versus modern findings. Forsch Komplementmed. 2010;17(5):241-8. https://doi.org/10.1159/000321397
  8. Lim SJ. Study on cancer patients presenting to the emergency department due to chemotherapy induced side effects. Department of Nursing, The Graduate school of Seoul National University. 2013.
  9. Lee SI. Herbology. Seoul:Suseowon. 1981:532-3.
  10. The compilation committee of Herbology. Herbology. Seoul:Younglim pubilisher. 2008:725.
  11. Sokoloff B. The oncostatic and oncolytic factors present in certain plants, Oncology. 1969;22:49-60.
  12. So JB, et al. The study on anticancer effect of Chelidonium majus. The Korea journal of herbology. 1997;13(2):63-72.
  13. Kim SC, et al. The Effects of Chelidonii Herba Extract on Apoptosis in Human Stomach Adenocarcinoma Cell Line. The korean journal of oriental medical prescription. 2005;13(1):71-83.
  14. Biswas SJ, Bhattacharjee N, Khuda-Bukhsh AR. Efficacy of a plant extract (Chelidonium majus L.) in combating induced hepatocarcinogenesis in mice. Food Chem Toxicol. 2008; 46(5):1474-87. https://doi.org/10.1016/j.fct.2007.12.009
  15. Nadova S, et al. Potential antioxidant activity, cytotoxic and apoptosis-inducing effects of Chelidonium majus L. extract on leukemia cells. Neuro Endocrinol Lett. 2008;29(5):649-52.
  16. Nawrot R, Wóluń-Cholewa M, Goździcka-Józefiak A. Nucleases isolated from Chelidonium majus L. milky sap can induce apoptosis in human cervical carcinoma HeLa cells but not in Chinese Hamster Ovary CHO cells. Folia Histochem Cytobiol. 2008;46(1) :79-83.
  17. Park SW, et al. Chelidonium majus L. extract induces apoptosis through caspase activity via MAPK-independent NF-κB signaling in human epidermoid carcinoma A431 cells. Oncol Rep. 2015; Epub 2014 Oct 23;33(1):419-24. https://doi.org/10.3892/or.2014.3566
  18. Kim JB, Ahn GS. Articles : A Study on the Pathology of Breast Canncer. The Journal of oriental medical pathology. 1994;9(1):189-208.
  19. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. Journal of Immunological Methods. 1983;65(1-2) :55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  20. Paik NS. Current Status of Breast Cancer. Journal of Korean Association of Cancer prevention. 2003;8(4):236-44.
  21. 巢元方. 巢氏諸病源候論. Seoul:DSprint. 1992:296.
  22. Lee JH, Yoo DY. Meta Analysis of researches about herbal extracts used in breast cancer in South Korea since 2000. Institute of Korean Medicine of Daejeon university. 2007;16(2):241-9.
  23. Kim JS, Lee TK, Kim DI. A study of antiproliferative effect by Euonymus alatus (Thunb.) Sieb water-extract on SKBR3 human breast cancer cell Line. J Korean Obstet Gynecol. 2005; 18(4):1-9.
  24. Yang DS, Yang SJ. Effects of Curcuma longa L. on MDA-MB-231 Human Breast Cancer Cells and DMBA-induced Breast Cancer in Rats. J Korean Obstet Gynecol. 2013;26(3):44-58. https://doi.org/10.15204/jkobgy.2013.26.3.044
  25. Park KM, Cho SH, Jeong KA. Anti-proliferative effect of Sam-nueng (Sparganii Rhizoma) extract on MCF-7 cells. J Korean Obstet Gynecol. 2006; 19(1):166-77.
  26. Jung S, et al. Inhibition of Cellular Proliferation by Curcumae Longae Rhizoma Extracts on MCF-7. The Korea journal of herbology. 2006;21(1) :71-7.
  27. Yoon WK, Kim DC. Toosendan Fructus Induces Apoptotic Cell Death in MCF-7 Cell, Via the Inhibition of Bcl-2 Expression. J Korean Obstet Gynecol. 2008;21(3):18-33.
  28. Park YA, Kim DC. Corydalis Tuber Induces Apoptosis in MCF-7 Cells, Via Inhibition of Bcl-2 and Bcl- XL Expression. J Korean Obstet Gynecol. 2008;21(4):90-103.
  29. Lee HJ, et al. Effects of Sophorae Radix on Human Breast Adenocarcinoma Cells. Korean journal of oriental medicine. 2012;18(1):75-84.
  30. Kim JN, et al. Effects of Schisandra Chinensis on Human Breast Cancer Cells. Korean journal of oriental physiology & pathology. 2014;28(2) :162-8.
  31. Colombo ML, Bosisio E. Pharmacological activities of Chelidonium majus L. (Papaveraceae). Pharmacol Res. 1996; 33(2):127-34. https://doi.org/10.1006/phrs.1996.0019
  32. Ernst E, Schmidt K. Ukrain - a new cancer cure? A systematic review of randomised clinical trials. BMC Cancer. 2005;5:69. https://doi.org/10.1186/1471-2407-5-69
  33. Sang ME. Juhaedosul Hangamboncho. Seoul:Iljoong publisher. 1992:204-5.
  34. Vinay Kumar, et al. Robbins Basic Pathology. 8e. Elsevier Inc:USA. 2007:19-23.
  35. Williams GT. Programmed cell death:apoptosis and oncogenesis. Cell. 1991; 65(7):1097-8. https://doi.org/10.1016/0092-8674(91)90002-G