Odontoclast and Osteoclast Formation in Rats with Ligature-Induced Periodontitis

치주염 유발 쥐에서 상아질파괴세포와 뼈파괴세포의 형성

  • Received : 2015.04.10
  • Accepted : 2015.05.06
  • Published : 2015.06.30


Odontoclasts and osteoclasts resorb tooth root and alveolar bone, respectively. Many studies have focused on osteoclast formation in periodontitis, but effect of periodontitis on odontoclast formation is not clearly clarified. In this study, we observed formation of odontoclasts as well as osteoclasts in rats with ligature-induced periodontitis. To induce periodontitis, ligatures were placed around the first molars in left mandibles of rats. Rats were sacrificed at days 1, 3, and 10 after ligation. After tartrate resistant acid phosphatase (TRAP) staining in mandible section, the number of TRAP-positive odontoclasts and osteoclasts were histologically counted along the root and the alveolar bone surfaces of tooth, respectively. Odontoclasts increased until day 10 in mesial and furcation root surface, but did not increase in distal root surface. When compared odontoclast formation to osteoclast formation in mesial surface, osteoclasts peaked at day 3, and then decreased gradually, whereas odontoclasts were continuously increased until day 10. The number of odontoclasts was lower than that of osteoclasts before and after periodontitis induction. These indicate that periodontitis increased formation of odontoclasts as well as osteoclasts, but odontoclast formation occurs slower and weaker than that of osteoclasts.




  1. Harokopakis-Hajishengallis E: Physiologic root resorption in primary teeth: molecular and histological events. J Oral Sci 49: 1-12, 2007.
  2. Sahara N, Toyoki A, Ashizawa Y, Deguchi T, Suzuki K: Cytodifferentiation of the odontoclast prior to the shedding of human deciduous teeth: an ultrastructural and cytochemical study. Anat Rec 244: 33-49, 1996.<33::AID-AR4>3.0.CO;2-G
  3. Muto A, Mizoguchi T, Udagawa N, et al.: Lineage-committed osteoclast precursors circulate in blood and settle down into bone. J Bone Miner Res 26: 2978-2990, 2011.
  4. Yamaguchi M, Aihara N, Kojima T, Kasai K: RANKL increase in compressed periodontal ligament cells from root resorption. J Dent Res 85: 751-756, 2006.
  5. Tyrovola JB, Spyropoulos MN, Makou M, Perrea D: Root resorption and the OPG/RANKL/RANK system: a mini review. J Oral Sci 50: 367-376, 2008.
  6. Crespo Abelleira AC, Rodriguez Cobos MA, Fuentes Boquete IM, Castano Oreja MT, Jorge Barreiro FJ, Rodriguez Pato RB: Morphological study of root surfaces in teeth with adult periodontitis. J Periodontol 70: 1283-1291, 1999.
  7. Rodriguez-Pato RB: Root resorption in chronic periodontitis: a morphometrical study. J Periodontol 75: 1027-1032, 2004.
  8. Nakano Y, Yamaguchi M, Fujita S, Asano M, Saito K, Kasai K: Expressions of RANKL/RANK and M-CSF/c-fms in root resorption lacunae in rat molar by heavy orthodontic force. Eur J Orthod 33: 335-343, 2011.
  9. Fukushima H, Kajiya H, Takada K, Okamoto F, Okabe K: Expression and role of RANKL in periodontal ligament cells during physiological root-resorption in human deciduous teeth. Eur J Oral Sci 111: 346-352, 2003.
  10. Kim JH, Lee DE, Cha JH, Bak EJ, Yoo YJ: Receptor activator of nuclear factor-$\kappa$B ligand and sclerostin expression in osteocytes of alveolar bone in rats with ligature-induced periodontitis. J Periodontol 85: e370-e378, 2014.
  11. Lee DE, Kim JH, Choi SH, Cha JH, Bak EJ, Yoo YJ: Periodontitis mainly increases osteoclast formation via enhancing the differentiation of quiescent osteoclast precursors into osteoclasts. J Periodontal Res 50: 256-264, 2015.
  12. Oka H, Mori M, Kihara H: F-spondin inhibits migration and differentiation of osteoclastic precursors. J Periodontol 82:1776-1783, 2011.
  13. Duarte PM, Tezolin KR, Figueiredo LC, Feres M, Bastos MF: Microbial profile of ligature-induced periodontitis in rats. Arch Oral Biol 55: 142-147, 2010.


Supported by : 연세대학교 치과대학