Preventive Effects of a Major Component of Green Tea, Epigallocathechin-3-Gallate, on Hepatitis-B Virus DNA Replication

  • Karamese, Murat (Department of Microbiology, Medical Faculty, Kafkas University) ;
  • Aydogdu, Sabiha (Department of Microbiology, Medical Faculty, Ataturk University) ;
  • Karamese, Selina Aksak (Department of Histology and Embryology, Medical Faculty, Kafkas University) ;
  • Altoparlak, Ulku (Department of Microbiology, Medical Faculty, Ataturk University) ;
  • Gundogdu, Cemal (Department of Pathology, Medical Faculty, Ataturk University)
  • Published : 2015.06.03


Background: Hepatitis B virus infection is one of the major world health problems. Epigallocatechin-3 gallate is the major component of the polyphenolic fraction of green tea and it has an anti-viral, anti-mutagenic, anti-tumorigenic, anti-angiogenic, anti-proliferative, and/or pro-apoptotic effects on mammalian cells. In this study, our aim was to investigate the inhibition of HBV replication by epigallocatechin-3 gallate in the Hep3B2.1-7 hepatocellular carcinoma cell line. Materials and Methods: HBV-replicating Hep3B2.1-7 cells were used to investigate the preventive effects of epigallocatechin-3 gallate on HBV DNA replication. The expression levels of HBsAg and HBeAg were determined using ELISA. Quantitative real-time-PCR was applied for the determination of the expression level of HBV DNA. Results: Cytotoxicity of epigallocathechin-3-gallate was not observed in the hepatic carcinoma cell line when the dose was lower than $100{\mu}M$. The ELISA method demonstrated that epigallocatechin-3 gallate have strong effects on HBsAg and HBeAg levels. Also it was detected by real-time PCR that epigallocatechin-3 gallate could prevent HBV DNA replication. Conclusions: The obtained data pointed out that although the exact mechanism of HBV DNA replication and related diseases remains unclear, epigallocatechin-3 gallate has a potential as an effective anti-HBV agent with low toxicity.


Hepatitis B;Hepatocellular carcinoma;EGCG;green tea;in-vitro


Supported by : Ataturk University


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