- Volume 16 Issue 12
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Relationship between Genetic Polymorphisms in MTHFR (C677T, A1298C and their Haplotypes) and the Incidence Of Breast Cancer among Jordanian Females - Case-Control Study
- Awwad, Nemah (Biopharmaceutics and Clinical Pharmacy, Department of Pharmacy, The University of Jordan) ;
- Yousef, Al-Motassem (Biopharmaceutics and Clinical Pharmacy, Department of Pharmacy, The University of Jordan) ;
- Abuhaliema, Ali (Biopharmaceutics and Clinical Pharmacy, Department of Pharmacy, The University of Jordan) ;
- Abdalla, Ihab (Biopharmaceutics and Clinical Pharmacy, Department of Pharmacy, The University of Jordan) ;
- Yousef, Muhammad (Biopharmaceutics and Clinical Pharmacy, Department of Pharmacy, The University of Jordan)
- Published : 2015.07.13
Background: Breast cancer is a major cause of morbidity and mortality in Jordan and worldwide. Abnormality of DNA methylation is a possible mechanism for the development of cancer. Methylenetetrahydrofolate reductase (MTHFR) is involved in DNA methylation. Our aim was to study the association between genetic polymorphisms of MTHFR at two sites (C677T and A1298C) and their haplotypes and the risk of breast cancer among Jordanian females. Materials and Methods: A case-control study involving 150 breast cancer cases and 150 controls was conducted. Controls were age-matched to cases. Polymerase chain reaction/restriction fragment length polymorphism (PCR-RFLP) technique and sequencing were conducted to determine the genotypes. Results: There was a significant difference in genotype frequency of C677T in the 41-60 year age category [cases: CC (37.4%), CT (49.5%) and TT (13.2%); controls: CC (56.3%), CT (35.6%) and TT (8%), p= 0.04;
Supported by : Deanship of Scientific Research (University of Jordan, Jordan)
- American Cancer Society. 2012. Cancer Facts and Figures 2012 [Online]. Available: http://www.cancer.org/acs/groups/content/@epidemiologysurveilance/documents/document/acspc-031941.pdf [Accessed 25-3-2015 2015].
- Butterworth AS, Higgins JP, Pharoah P (2006). Relative and absolute risk of colorectal cancer for individuals with a family history: a meta-analysis. Eur J Cancer, 42, 216-27. https://doi.org/10.1016/j.ejca.2005.09.023
- Chad M. Barnett, Laura Boehnke Michaud, Francisco J. Esteva (2014). Bresat cancer. In 'Pharmacotherapy: A Pathophysiologic Approach', Eds Mc Graw Hill education,
Cochran G (1954). Some methods for strengthening the common
$\chi$tests. Biometrics, 10.
- Collaborative Group on Hormonal Factors in Breast Cancer (2001). Familial breast cancer: collaborative reanalysis of individual data from 52 epidemiological studies including 58,209 women with breast cancer and 101,986 women without the disease. Lancet, 358, 1389-99. https://doi.org/10.1016/S0140-6736(01)06524-2
- Diakite B, Tazzite A, Hamzi K, et al (2012). Methylenetetrahydrofolate reductase C677T polymorphism and breast cancer risk in Moroccan women. African Health Sciences, 12, 204-9.
- Eliassen AH, Colditz GA, Rosner B, et al (2006). Adult weight change and risk of postmenopausal breast cancer. JAMA, 296, 193-201. https://doi.org/10.1001/jama.296.2.193
- Food Fortification Initiative. 2014. Middle East - Fortification Status [Online]. Available: http://www.ffinetwork.org/regional_activity/middle_east.php#middle_east.php?s=2&_suid=142601829808809411473741271781 [Accessed 10-3-2015 2015].
- Foulkes WD (2008). Inherited susceptibility to common cancers. N Eng J Med, 359, 2143-53. https://doi.org/10.1056/NEJMra0802968
- Frosst P, Blom HJ, Milos R, et al (1995). A candidate genetic risk factor for vascular disease: a common mutation in methylenetetrahydrofolate reductase. Nature Genetics, 10, 111-3. https://doi.org/10.1038/ng0595-111
- Gaughan DJ, Barbaux S, Kluijtmans LA, et al (2000). The human and mouse methylenetetrahydrofolate reductase (MTHFR) genes: genomic organization, mRNA structure and linkage to the CLCN6 gene. Gene, 257, 279-89. https://doi.org/10.1016/S0378-1119(00)00392-9
- Hosseini M, Houshmand M, Ebrahimi A (2011). MTHFR polymorphisms and breast cancer risk. Arch Medical Science, 7, 134-7.
- Jiang Y, Hou J, Zhang Q, et al (2013). The MTHFR C677T polymorphism and risk of acute lymphoblastic leukemia: an updated meta-analysis based on 37 case-control studies. Asian Pac J Cancer Prev, 14, 6357-62. https://doi.org/10.7314/APJCP.2013.14.11.6357
- Jiao Z, Li D (2013). Lack of association between MHTFR Glu429Ala polymorphism and breast cancer susceptibility: a systematic review and meta-analysis of 29 research studies. Tumour Biology, 34, 1225-33. https://doi.org/10.1007/s13277-013-0665-7
- Jordan ministry of health. 2010. Annual Incidence of cancer in Jordan [Online]. Available: http://www.moh.gov.jo/EN/Pages/Periodic-Newsletters.aspx [Accessed 21-1-2015 2015].
- Kruk J (2014). Lifestyle Components and Primary Breast Cancer Prevention. Asian Pac J Cancer Prev, 15, 10543-55.
- Liew S, Gupta ED (2015). Methylenetetrahydrofolate reductase (MTHFR) C677T polymorphism: Epidemiology, metabolism and the associated diseases. Eur J Medical Genetics, 58, 1-10. https://doi.org/10.1016/j.ejmg.2014.10.004
- Luo B, Repalli J, Yousef AM, et al (2011). Human thymidylate synthase with loop 181-197 stabilized in an inactive conformation: ligand interactions, phosphorylation, and inhibition profiles. Protein Science, 20, 87-94. https://doi.org/10.1002/pro.539
- Marie A., Barbara G., Terry L., et al 2013. Pharmacotherapy Principles and Practice.
- National cancer institute. 2014. BRCA1 and BRCA2: Cancer Risk and Genetic Testing [Online]. Available: http://www.cancer.gov/cancertopics/genetics/brca-fact-sheet [Accessed 6-3-2015 2015].
- Ozen F, Sen M, Ozdemir O (2014). Methylenetetrahydrofolate reductase gene germ-line C677T and A1298C SNPs are associated with colorectal cancer risk in the Turkish population. Asian Pac J Cancer Prev, 15, 7731-5. https://doi.org/10.7314/APJCP.2014.15.18.7731
- Pharoah PD, Day NE, Duffy S, et al (1997). Family history and the risk of breast cancer: a systematic review and meta-analysis. Intl J Cancer, 71, 800-9. https://doi.org/10.1002/(SICI)1097-0215(19970529)71:5<800::AID-IJC18>3.0.CO;2-B
- Rai V (2014a). Methylenetetrahydrofolate Reductase A1298C Polymorphism and Breast Cancer Risk: A Meta-analysis of 33 Studies. Ann Medical Health Sciences Res, 4, 841-51. https://doi.org/10.4103/2141-9248.144873
- Rai V (2014b). The methylenetetrahydrofolate reductase C677T polymorphism and breast cancer risk in Asian populations. Asian Pac J Cancer Prev, 15, 5853-60. https://doi.org/10.7314/APJCP.2014.15.14.5853
- Rodriguez S, Gaunt TR, Day IN (2009). Hardy-Weinberg equilibrium testing of biological ascertainment for Mendelian randomization studies. Am J Epidemiol, 169, 505-14. https://doi.org/10.1093/aje/kwn359
- Shiovitz S, Korde LA (2015). Genetics of breast cancer: a topic in Evolution. Ann Oncol. [Epub ahead of print]
- Siegel R, Naishadham D, Jemal A (2012). Cancer statistics, 2012. CA: a Cancer J Clin, 62, 10-29. https://doi.org/10.3322/caac.20138
- Walker SE, Lorsch J (2013). Sanger dideoxy sequencing of DNA. Method Enzymol, 529, 171-84. https://doi.org/10.1016/B978-0-12-418687-3.00014-8
- Welcsh PL, King MC (2001). BRCA1 and BRCA2 and the genetics of breast and ovarian cancer. Human Molecular Genetics, 10, 705-13. https://doi.org/10.1093/hmg/10.7.705
- Xu X, Gammon MD, Zeisel SH, et al (2008). Choline metabolism and risk of breast cancer in a population-based study. FASEB J, 22, 2045-52. https://doi.org/10.1096/fj.07-101279
- Yang YB, Shang YH, Tan YL, et al (2014). Methylenetetrahydrofolate reductase polymorphisms and susceptibility to esophageal cancer in Chinese populations: a meta-analysis. Asian Pac J Cancer Prev, 15, 1345-9. https://doi.org/10.7314/APJCP.2014.15.3.1345
- Yousef AM, Shomaf M, Berger S, et al (2013). Allele and genotype frequencies of the polymorphic methylenetetrahydrofolate reductase and colorectal cancer among Jordanian population. Asian Pac J Cancer Prev, 14, 4559-65. https://doi.org/10.7314/APJCP.2013.14.8.4559
- Zhang, Qiu LX, Wang ZH, et al (2010). MTHFR C677T polymorphism associated with breast cancer susceptibility: a meta-analysis involving 15,260 cases and 20,411 controls. Breast Cancer Res Treatment, 123, 549-55. https://doi.org/10.1007/s10549-010-0783-5
- Zhong S, Chen Z, Yu X, et al (2014). A meta-analysis of genotypes and haplotypes of methylenetetrahydrofolate reductase gene polymorphisms in breast cancer. Molec Cell Biochem, 41, 5775-85.
- Zintzaras E (2006). Methylenetetrahydrofolate reductase gene and susceptibility to breast cancer: a meta-analysis. Clinical Genetics, 69, 327-36. https://doi.org/10.1111/j.1399-0004.2006.00605.x
- Zintzaras E, Koufakis T, Ziakas PD, et al (2006). A meta-analysis of genotypes and haplotypes of methylenetetrahydrofolate reductase gene polymorphisms in acute lymphoblastic leukemia. Eur J Epidemiol, 21, 501-10. https://doi.org/10.1007/s10654-006-9027-8
- Zintzaras E, Lau J (2008). Synthesis of genetic association studies for pertinent gene-disease associations requires appropriate methodological and statistical approaches. J Clin Epidemiol, 61, 634-45. https://doi.org/10.1016/j.jclinepi.2007.12.011
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- Population-level diversity in the association of genetic polymorphisms of one-carbon metabolism with breast cancer risk vol.7, pp.4, 2016, https://doi.org/10.1007/s12687-016-0277-1
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