- Volume 16 Issue 12
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Relationship between Genetic Polymorphisms in MTHFR (C677T, A1298C and their Haplotypes) and the Incidence Of Breast Cancer among Jordanian Females - Case-Control Study
- Awwad, Nemah (Biopharmaceutics and Clinical Pharmacy, Department of Pharmacy, The University of Jordan) ;
- Yousef, Al-Motassem (Biopharmaceutics and Clinical Pharmacy, Department of Pharmacy, The University of Jordan) ;
- Abuhaliema, Ali (Biopharmaceutics and Clinical Pharmacy, Department of Pharmacy, The University of Jordan) ;
- Abdalla, Ihab (Biopharmaceutics and Clinical Pharmacy, Department of Pharmacy, The University of Jordan) ;
- Yousef, Muhammad (Biopharmaceutics and Clinical Pharmacy, Department of Pharmacy, The University of Jordan)
- Published : 2015.07.13
Background: Breast cancer is a major cause of morbidity and mortality in Jordan and worldwide. Abnormality of DNA methylation is a possible mechanism for the development of cancer. Methylenetetrahydrofolate reductase (MTHFR) is involved in DNA methylation. Our aim was to study the association between genetic polymorphisms of MTHFR at two sites (C677T and A1298C) and their haplotypes and the risk of breast cancer among Jordanian females. Materials and Methods: A case-control study involving 150 breast cancer cases and 150 controls was conducted. Controls were age-matched to cases. Polymerase chain reaction/restriction fragment length polymorphism (PCR-RFLP) technique and sequencing were conducted to determine the genotypes. Results: There was a significant difference in genotype frequency of C677T in the 41-60 year age category [cases: CC (37.4%), CT (49.5%) and TT (13.2%); controls: CC (56.3%), CT (35.6%) and TT (8%), p= 0.04;
Jordanian females;MTHFR;polymorphism;breast cancer;risk factor
Supported by : Deanship of Scientific Research (University of Jordan, Jordan)
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