Soluble extract of soybean fermented with Aspergillus oryzae GB107 inhibits fat accumulation in cultured 3T3-L1 adipocytes

  • So, Kyoung-Ha (Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University) ;
  • Suzuki, Yasuki (Faculty of Agriculture, Shinshu University) ;
  • Yonekura, Shinichi (Faculty of Agriculture, Shinshu University) ;
  • Suzuki, Yutaka (Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University) ;
  • Lee, Chan Ho (Genebiotech Co.) ;
  • Kim, Sung Woo (Department of Animal Science, North Carolina State University) ;
  • Katoh, Kazuo (Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University) ;
  • Roh, Sang-Gun (Laboratory of Animal Physiology, Graduate School of Agricultural Science, Tohoku University)
  • Received : 2014.11.26
  • Accepted : 2015.02.16
  • Published : 2015.08.01


BACKGROUND/OBJECTIVES: This study was conducted to investigate the effects of fermented soybean (FS) extract on adipocyte differentiation and fat accumulation using cultured 3T3-L1 adipocytes. MATERIALS/METHODS: 3T3-L1 adipocytes were treated with FS and nonfermented soybean (NFS) extract during differentiation for 10 days in vitro. Oil red O staining was performed and glycerol-3-phosphate dehydrogenase (GPDH) activity was measured for analysis of fat accumulation. Expressions of adipogenic genes were measured. RESULTS: Soluble extract of soybean fermented with Aspergillus oryzae GB107 contained higher levels of low-molecular-weight protein than conventional soybean protein did. FS extract ($50{\mu}g/ml$) inhibited adipocyte differentiation and fat accumulation during differentiation of 3T3-L1 preadipocytes for 10 days in vitro. Significantly lower GPDH activity was observed in differentiated adipocytes treated with the FS extract than those treated with NFS extract. Treatment with FS extract resulted in decreased expression levels of leptin, adiponectin, and adipogenin genes, which are associated with adipogenesis. CONCLUSIONS: This report is the first to demonstrate that the water-soluble extract from FS inhibits fat accumulation and lipid storage in 3T3-L1 adipocytes. Thus, the soybean extract fermented with A. oryzae GB107 could be used to control lipid accumulation in adipocytes.


Supported by : JSPS KAKENHI


  1. Hong KJ, Lee CH, Kim SW. Aspergillus oryzae GB-107 fermentation improves nutritional quality of food soybeans and feed soybean meals. J Med Food 2004;7:430-5.
  2. Kim SW, van Heugten E, Ji F, Lee CH, Mateo RD. Fermented soybean meal as a vegetable protein source for nursery pigs: I. Effects on growth performance of nursery pigs. J Anim Sci 2010;88:214-24.
  3. Roh SG, Carroll JA, Kim SW. Effects of fermented soybean meal on innate immunity-related gene expressions in nursery pigs acutely challenged with lipopolysaccharides. Anim Sci J. Forthcoming 2014.
  4. Friedman JM, Halaas JL. Leptin and the regulation of body weight in mammals. Nature 1998;395:763-70.
  5. Gregoire FM, Smas CM, Sul HS. Understanding adipocyte differentiation. Physiol Rev 1998;78:783-809.
  6. Roh SG, Hishikawa D, Hong YH, Sasaki S. Control of adipogenesis in ruminants. Anim Sci J 2006;77:472-7.
  7. Zhang Y, Proenca R, Maffei M, Barone M, Leopold L, Friedman JM. Positional cloning of the mouse obese gene and its human homologue. Nature 1994;372:425-32.
  8. Sethi JK, Xu H, Uysal KT, Wiesbrock SM, Scheja L, Hotamisligil GS. Characterisation of receptor-specific TNFalpha functions in adipocyte cell lines lacking type 1 and 2 TNF receptors. FEBS Lett 2000;469: 77-82.
  9. Yamauchi T, Kamon J, Ito Y, Tsuchida A, Yokomizo T, Kita S, Sugiyama T, Miyagishi M, Hara K, Tsunoda M, Murakami K, Ohteki T, Uchida S, Takekawa S, Waki H, Tsuno NH, Shibata Y, Terauchi Y, Froguel P, Tobe K, Koyasu S, Taira K, Kitamura T, Shimizu T, Nagai R, Kadowaki T. Cloning of adiponectin receptors that mediate antidiabetic metabolic effects. Nature 2003;423:762-9.
  10. Hotamisligil GS, Shargill NS, Spiegelman BM. Adipose expression of tumor necrosis factor-alpha: direct role in obesity-linked insulin resistance. Science 1993;259:87-91.
  11. Roh SG, Song SH, Choi KC, Katoh K, Wittamer V, Parmentier M, Sasaki S. Chemerin--a new adipokine that modulates adipogenesis via its own receptor. Biochem Biophys Res Commun 2007;362:1013-8.
  12. Suzuki Y, Hong YH, Song SH, Ardiyanti A, Kato D, So KH, Katoh K, Roh SG. The regulation of chemerin and CMKLR1 genes expression by TNF-$\alpha$, adiponectin, and chemerin analog in bovine differentiated adipocytes. Asian-Australas J Anim Sci 2012;25: 1316-21.
  13. Suzuki Y, Song SH, Sato K, So KH, Ardiyanti A, Kitayama S, Hong YH, Lee SD, Choi KC, Hagino A, Katoh K, Roh SG. Chemerin analog regulates energy metabolism in sheep. Anim Sci J 2012;83:263-7.
  14. Tontonoz P, Hu E, Spiegelman BM. Stimulation of adipogenesis in fibroblasts by PPAR gamma 2, a lipid-activated transcription factor. Cell 1994;79:1147-56.
  15. Kwon DY, Daily JW 3rd, Kim HJ, Park S. Antidiabetic effects of fermented soybean products on type 2 diabetes. Nutr Res 2010; 30:1-13.
  16. Hong YH, Hishikawa D, Miyahara H, Tsuzuki H, Nishimura Y, Gotoh C, Choi KC, Hokari Y, Takagi Y, Lee HG, Cho KK, Roh SG, Sasaki S. Up-regulation of adipogenin, an adipocyte plasma transmembrane protein, during adipogenesis. Mol Cell Biochem 2005;276: 133-41.
  17. Kim HK, Kim JN, Han SN, Nam JH, Na HN, Ha TJ. Black soybean anthocyanins inhibit adipocyte differentiation in 3T3-L1 cells. Nutr Res 2012;32:770-7.
  18. Choi KC, Shrestha YB, Roh SG, Hishikawa D, Kuno M, Tsuzuki H, Hong YH, Sasaki S. The role of phosphatidylinositol 3-kinase and mitogenic activated protein kinase on the differentiation of ovine preadipocytes. Asian-Australas J Anim Sci 2003;16:1199-204.
  19. Hong YH, Nishimura Y, Hishikawa D, Tsuzuki H, Miyahara H, Gotoh C, Choi KC, Feng DD, Chen C, Lee HG, Katoh K, Roh SG, Sasaki S. Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR43. Endocrinology 2005;146:5092-9.
  20. Wise LS, Green H. Participation of one isozyme of cytosolic glycerophosphate dehydrogenase in the adipose conversion of 3T3 cells. J Biol Chem 1979;254:273-5.
  21. Gonzalez de Mejia E, Martinez-Villaluenga C, Roman M, Bringe NA. Fatty acid synthase and in vitro adipogenic response of human adipocytes inhibited by $\alpha$ and ${\alpha}{\prime}$ subunits of soybean $\beta$-conglycinin hydrolysates. Food Chem 2010;119:1571-7.
  22. Martinez-Villaluenga C, Bringe NA, Berhow MA, Gonzalez de Mejia E. $\beta$-Conglycinin embeds active peptides that inhibit lipid accumulation in 3T3-L1 adipocytes in vitro. J Agric Food Chem 2008;56:10533-43.
  23. Lovati MR, Manzoni C, Canavesi A, Sirtori M, Vaccarino V, Marchi M, Gaddi G, Sirtori CR. Soybean protein diet increases low density lipoprotein receptor activity in mononuclear cells from hypercholesterolemic patients. J Clin Invest 1987;80:1498-502.
  24. Oakenfull DG, Topping DL. Saponins and plasma cholesterol. Atherosclerosis 1983;48:301-3.
  25. Sidhu GS, Oakenfull DG. A mechanism for the hypocholesterolaemic activity of saponins. Br J Nutr 1986;55:643-9.
  26. Sugano M, Goto S, Yamada Y, Yoshida K, Hashimoto Y, Matsuo T, Kimoto M. Cholesterol-lowering activity of various undigested fractions of soybean protein in rats. J Nutr 1990;120:977-85.
  27. Kwon DY, Hong SM, Ahn IS, Kim MJ, Yang HJ, Park S. Isoflavonoids and peptides from meju, long-term fermented soybeans, increase insulin sensitivity and exert insulinotropic effects in vitro. Nutrition 2011;27:244-52.
  28. Iwai K, Nakaya N, Kawasaki Y, Matsue H. Inhibitory effect of natto, a kind of fermented soybeans, on LDL oxidation in vitro. J Agric Food Chem 2002;50:3592-6.
  29. Iwai K, Nakaya N, Kawasaki Y, Matsue H. Antioxidative functions of natto, a kind of fermented soybeans: effect on LDL oxidation and lipid metabolism in cholesterol-fed rats. J Agric Food Chem 2002;50:3597-601.
  30. de Mejia EG, Dia VP. Lunasin and lunasin-like peptides inhibit inflammation through suppression of NF-kappaB pathway in the macrophage. Peptides 2009;30:2388-98.

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