Lifestyle intervention might easily improve blood pressure in hypertensive men with the C genotype of angiotensin II type 2 receptor gene

  • Kitaoka, Kaori (Department of Health and Nutrition, Faculty of Health Science, Kyoto Koka Women's University) ;
  • Kitade, Azusa (Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University) ;
  • Nagaoka, Junko (Health Promotion Division, Higashiosaka City Public Health Office) ;
  • Tsuzaki, Kokoro (Division of Preventive Medicine and Diabetes Education, Clinical Research Institute, National Hospital Organization Kyoto Medical Center) ;
  • Harada, Kiyomi (School of Nursing, Kyoto Prefectural University of Medicine) ;
  • Aoi, Wataru (Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University) ;
  • Wada, Sayori (Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University) ;
  • Asano, Hiroaki (School of Nursing, Kyoto Prefectural University of Medicine) ;
  • Sakane, Naoki (Division of Preventive Medicine and Diabetes Education, Clinical Research Institute, National Hospital Organization Kyoto Medical Center) ;
  • Higashi, Akane (Division of Applied Life Sciences, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University)
  • Received : 2014.06.20
  • Accepted : 2015.03.09
  • Published : 2015.08.01


BACKGROUND/OBJECTIVES: Recent studies have reported an association of the angiotensin II type 2 receptor (AT2R) 3123Cytosine/Adenine (3123C/A) polymorphism with essential hypertension and cardiovascular diseases. The purpose of the study was to investigate whether the AT2R 3123C/A polymorphism affects blood pressure for free-living hypertensive men during a 5-month intervention period. SUBJECTS/METHODS: The subjects were free-living hypertensive Japanese men aged 40 to 75 years who agreed to intervention in the period from 2004 to 2011. Detection of the AT2R 3123C/A polymorphism was determined by polymerase chain reaction. The dietary intervention was designed to decrease salt level and to increase potassium level through cooking instructions and self-monitoring of the diet. The exercise session consisted of activities such as stretching, resistance training, and walking. Blood pressure, urinary sodium and potassium excretion, dietary and lifestyle data, and non-fasting venous blood sample were collected at baseline and after the intervention period. RESULTS: Thirty nine subjects were eligible for participation and the follow-up rate was 97.4%. The C allele proportion was 57.9%. AT2R 3123C/A polymorphism was X-chromosome-linked, therefore we analyzed the C and A genotypes. At baseline, no significant differences were observed between the genotype groups. After the intervention, there were no significant differences in lifestyle habit between the groups. Nevertheless, the estimated salt excretion (g/day) was significantly decreased only in the C genotype (13.0-10.3, P = 0.031). No significant change was observed in systolic blood pressure (SBP) (mmHg) in the A genotype, but a significant decrease was observed in the C genotype (150.0-141.5, P = 0.024). CONCLUSTIONS: In the C genotype, it might be easy to improve SBP through lifestyle intervention in free-living hypertensive Japanese men, however generalization could not be achieved by the small sample size.


Supported by : Health Promotion Foundation Japan, Osaka Gas Group Welfare Foundation


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