Characteristics of nonalcoholic fatty liver disease induced in wistar rats following four different diets

  • Fakhoury-Sayegh, Nicole (Department of Nutrition, Faculty of Pharmacy, Saint Joseph University) ;
  • Trak-Smayra, Viviane (Department of Pathology, Faculty of Medicine, Saint Joseph University) ;
  • Khazzaka, Aline (Department of Surgical Research, Faculty of Medicine, Saint Joseph University) ;
  • Esseily, Fady (Department of Laboratory Sciences, Faculty of Public Health II, Lebanese University) ;
  • Obeid, Omar (Department of Nutrition and Food Sciences, Faculty of Agricultural and Food Sciences, American University of Beirut) ;
  • Lahoud-Zouein, May (Department of Laboratory Sciences, Faculty of Public Health II, Lebanese University) ;
  • Younes, Hassan (Department of Nutrition and Health Sciences, Institut Polytechnique LaSalle Beauvais)
  • Received : 2014.05.20
  • Accepted : 2015.02.11
  • Published : 2015.08.01


BACKGROUND/OBJECTIVES: The prevalence of nonalcoholic fatty liver disease (NAFLD) has increased worldwide in parallel with overnutrition characterized by high-fat and high-carbohydrate intake. Our objective was to establish, in 16 weeks, a model of NAFLD in Wistar pathogen-free rats following four dietary types. MATERIALS/METHODS: Forty (6 weeks old) healthy Wistar male rats, weighing an average of 150 g were randomly divided into four groups of ten and assigned a diet with the same quantity (15 g/rat/day), but with different composition. The moderate-fat (MF) group was fed a moderate-fat diet (31.5% fat and 50% carbohydrates), the high-fat (HF) group was fed a fat-rich diet (51% fat), the high-sucrose (HS) group and the high-fructose (HFr) group were fed a carbohydrate-rich diet (61%). The carbohydrate contents of the HS group was composed of 60.3% sucrose while that of the HFr group was composed of 59.3% fructose. RESULTS: At week 16, the HF group had the highest percentage of cells enriched in fat (40%) and the highest weight and liver weight (P < 0.05). The HFr group showed significantly higher levels of serum triglycerides, alanine aminotransferase and adiponectin at week 16 as compared to week 1 (P < 0.05). CONCLUSIONS: The 15 g/rat/day diet composed of 51% fat or 61% carbohydrates enriched mainly in fructose may induce characteristics of NAFLD in rats.


Supported by : Saint Joseph University


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