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Effects of Cosmetics and Their Preservatives on the Growth and Composition of Human Skin Microbiota

피부 미생물총의 생장과 구성에 대한 화장품과 그 방부제의 영향

  • Jeong, Jin-Ju (Department of Life and Nanopharmaceutical Sciences, Kyung Hee University) ;
  • Kim, Dong-Hyun (Department of Life and Nanopharmaceutical Sciences, Kyung Hee University)
  • 정진주 (경희대학교 나노의약생명과학과) ;
  • 김동현 (경희대학교 나노의약생명과학과)
  • Received : 2015.03.06
  • Accepted : 2015.06.16
  • Published : 2015.06.30

Abstract

We investigated the growth-inhibitory activities of cosmetics and their preservatives against pathogens and resident skin bacteria. Of the tested cosmetics, preservatives such as parabens, 1,2-hexanediol, phenoxyethanol-contained toner, emulsion, cream and baby cream exhibited potent antibacterial effects against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Parabens, 1,2-hexanediol and phenoxyethanol inhibited the growth of pathogens, as well as skin-resident bacteria such as Staphilococcus epidermidis, Shigella flexneri, Enterobacter aerogenes and so on. The application of a basic cream containing phenoxyethanol to human skin was shown to disturb the skin microbiota: at the phylum level, Proteobacteria increased and at species level, 4P004125_s increased and Propionibacterium humerusii decreased. Based on these findings, parabens, 1,2-hexanediol and phenoxyethanol have antimicrobial activity and cosmetics containing phenoxyethanol may disturb skin microbiota.

화장품 및 화장품 함유 방부제에 대한 병원미생물과 사람의 피부서식세균들의 증식에 미치는 효과를 조사하였다. 파라벤, 1,2-헥산디올, 페녹시 에탄올과 같은 방부제를 함유한 토너, 에멀젼, 크림, 유아용크림은 포도상구균, 대장균, 녹농균에 대해 강한 방부효과를 보였으며, 파라벤, 1,2-헥산디올, 페녹시 에탄올과 같은 방부제들은 포도상구균, 대장균, 녹농균과 같은 병원균 및 Staphilococcus epidermidis, Shigella flexneri, Enterobacter aerogenes 등과 같은 일부 피부서식세균에 대한 강한 항균효과를 보였다. 사람의 피부에 페녹시 에탄올 함유 크림을 도포하였을 때, 피부서식 미생물들의 구성이 바뀌었다. 문 수준에서는 Proteobacteria가 증가하였고, 종 수준에서는 4P004125_s가 증가하였고, Propionibacterium humerusii 감소하였다. 이 결과들로부터 파라벤, 1,2-헥산디올, 페녹시 에탄올과 같은 방부제는 병원균에 대한 항균효과를 가짐과 동시에 페녹시 에탄올을 함유한 화장품은 피부세균군집에 변화를 줄 수 있음을 시사하고 있다.

Keywords

Acknowledgement

Supported by : Ministry of Food and Drug Safety

References

  1. J. A. Sanford and R. L. Gallo, Functions of the skin microbiota in health and disease, Semin Immunol., 25(5), 370 (2013).
  2. L. K. Ursell, J. C. Clemente, J. R. Rideout, D. Gevers, J. G. Caporaso, and R. Knight, The interpersonal and intrapersonal diversity of human-associated microbiota in key body sites, J. Allergy, Clin Immunol., 129(5), 1204 (2012). https://doi.org/10.1016/j.jaci.2012.03.010
  3. N. N. Schommer and R. L. Gallo, Structure and function of the human skin microbiome, Trends Microbiol., 21(12), 660 (2013). https://doi.org/10.1016/j.tim.2013.10.001
  4. P. L. Zeeuwen, M. Kleerebezem, H. M. Timmerman, and J. Schalkwijk, Microbiome and skin diseases, Curr. Opin, Allergy Clin. Immunol., 13(5), 514 (2013). https://doi.org/10.1097/ACI.0b013e328364ebeb
  5. Y. Belkaid and T. W. Hand, Role of the microbiota in immunity and inflammation, Cell., 157(1), 121 (2014). https://doi.org/10.1016/j.cell.2014.03.011
  6. A. Herman, A. P. Herman, B. W. Domagalska, and A. Mynarczyk, Essential oils and herbal extracts as antimicrobial agents in cosmetic emulsion, Indian J. Microbiol., 53(2), 232 (2013). https://doi.org/10.1007/s12088-012-0329-0
  7. R. I. AminovI, J. C. Chee-Sanford, N. Garrigues, A. ehboob, and R. I. Mackie, Detection of tetracycline resistance genes by PCR methods, Methods Mol. Biol., 268, 3 (2004).
  8. A. F. Kaul and J. F. Jewett, Agents and techniques for disinfection of the skin, Surg. Gynecol. Obstet., 152(5), 677 (1981).
  9. S. Kokura, O. Handa, T. Takagi, T. Ishikawa, Y. Naito, and T. Yoshikawa, Silver nanoparticles as a safe preservative for use in cosmetics, Nanomedicine., 6(4), 570 (2010). https://doi.org/10.1016/j.nano.2009.12.002
  10. R. Herruzo, M. J. Vizcaino, and I. Herruzo, In vitro-in vivo sequence studies as a method of selecting the most efficacious alcohol-based solution for hygienic hand disinfection, Clin Microbiol Infect., 16(5), 518 (2010). https://doi.org/10.1111/j.1469-0691.2009.02827.x
  11. J. H. Platt and R. A. Bucknall, MIC tests are not suitable for assessing antiseptic handwashes, J. Hosp. Infect., 11(4), 396 (1988). https://doi.org/10.1016/0195-6701(88)90095-3
  12. K. A. Kim, J. H. Jung, S. H. Park, Y. T. Ahn, C. S. Huh, and D. H. Kim, Comparative analysis of the gut microbiota in people with different levels of ginsenoside Rb1 degradation to compound K, PLoS One., 8, e62409. (2013). https://doi.org/10.1371/journal.pone.0062409
  13. G. Baviera, M. C. Leoni, L. Capra, F. Cipriani, G. Longo, N. Maiello, G. Ricci, and E. Galli, Microbiota in healthy skin and in atopic eczema Biomed. Res. Int., 2014, 436921 (2014).
  14. T. Jagielski, E. Rup, A. Ziolkowska, K. Roeske, A. B. Macura, and J. Bielecki, Distribution of Malassezia species on the skin of patients with atopic dermatitis, psoriasis, and healthy volunteers assessed by conventional and molecular identification methods, BMC Dermatol., 14, 3 (2014). https://doi.org/10.1186/1471-5945-14-3
  15. A. L. Cogen, K. Yamasaki, K. M. Sanchez, R. A. Dorschner, Y. Lai, D. T. MacLeod, J. W. Torpey, M. Otto, V. Nizet, J. E. Kim, and R. L. Gallo, Selective antimicrobial action is provided by phenol-soluble modulins derived from Staphylococcus epidermidis, a normal resident of the skin, J. Invest. Dermatol., 130, 192 (2010). https://doi.org/10.1038/jid.2009.243