• Received : 2015.04.16
  • Accepted : 2015.06.19
  • Published : 2015.06.30


We have employed X-ray photoelectron spectroscopy (XPS) technique to examine the combined effects of low-energy electron (LEE) irradiation and $Fe^{3+}$ ion on DNA damage. pBR322 plasmid DNA extracted from E. coli ER2420 was used for preparing DNA-$Fe^{3+}$ sample. The C1s XPS spectra were scanned for LEE-irradiated and LEE-unirradiated samples and then curve-fitted. For the samples with LEE irradiation only or with Fe ion only, no significant changes from pure DNA samples were observed - a single effect of either $Fe^{3+}$ ion or LEE irradiation did not cause a significant damage. However, when these two components were combined, the DNA damage was increased quite significantly, compared to the sum of DNA damages caused by $Fe^{3+}$ ion and by LEE irradiation independently. This observation is consistent with our previous results [Radiat. Res. 177, 775 (2012)] which was done using gel-electrophoresis technique. Partial interpretation of the observed spectrum peaks was also attempted.


Grant : 미래선도 플라즈마-농식품 융합기술 개발 사업


  1. Boudaiffa B, Cloutier P, Hunting D, Huels MA, Sanche L. Resonant formation of DNA strand breaks by low-energy(3 to 20 eV) electrons. Science. 2000;287:1658-1660.
  2. Sanche L. Low energy electron-driven damage in biomolecules. Eur Phys J D. 2005;35:367-390.
  3. Huels MA, Parenteau L, Sanche L. Reactive scattering of 1-5 eV $O^-$ in films of tetrahydrofuran. J Phys Chem B. 2004;108:16303-16312.
  4. Barrios R, Skurski P, Simons J. Mechanism for damage to DNA by low-energy electrons. J Phys Chem B. 2002;106:7991-7994.
  5. Ptasinska S, Sanche L. On the mechanism of anion desorption from DNA induced by low energy electrons. J Chem Phys. 2006;125:144713.
  6. Berdys J, Anusiewics I, Skurski P, Simons J. Damage to model DNA fragments from very low-energy(<1 eV) electrons. J Am Chem Soc. 2004;126:6441.
  7. Martin F, Burrow PD, Cai Z, Cloutier P, Hunting D, Sanche L. DNA strand breaks induced by 0-4 eV electrons: The role of shape resonances. Phys Rev Lett. 2004;93:068101.
  8. Lu QB, Sanche L. Condensed-phase effects ob absolute cross sections for dissociative electron attachment to CFCs and FCFCs adsorbed on Kr. J Chem Phys. 2003;119:2658-2662.
  9. Lu QB, Sanche L. Enhancement in dissociative electron attachment to $CF_4$, chlorofluorocarbons and hydrofluorocarbons adsorbed on $H_2O$ ice. J Chem Phys. 2004;120:2434-2438.
  10. Christophorou LG, McCorkle DL, Christodoulides AA. Electron-Molecule Interactions and Their Applications. New York; Academic Press. 1984.
  11. Chutjian A, Garscadden A, Wadehra JM. Electron attachment to molecules at low electron energies. Phys Rep. 1996;264:393-470.
  12. Ying ZC, Ho WJ. Photodissociation of adsorbed $MO(CO)_6$ induced by direct photoexcitation and hot-electro attachment. 2. Physical mechanisms. J Chem Phys. 1991;94:5701-5714.
  13. Sanche L. Nanoscopic aspects of electronic aging in dielectrics. IEEE Trans Dielectr Electr Insul. 1997;4:507-543.
  14. Lu QB, Sanche L. Effects of cosmic rays on atmospheric chlorofluorocarbon dissociation and ozone depletion. Phys Rev Lett. 2001;87:078501.
  15. Park YS, Cho H, Parenteau L, Bass AD, Sanche L. Cross sections for electron trapping by DNA and its component subunits I: condensed tetrahydrofuran deposited on Kr. J Chem Phys. 2006;125: 074714.
  16. Voet D, Voet JG, Pratt CW. Fundamentals of Biochemistry. New York; Wiley. 1999.
  17. Park Y, Noh H-A, Cho H. Effect of low-energy electron irradiation on DNA damage by $Fe^{3+}$ ion. Radiation Res. 2012;177;775-780.
  18. Aruoma OI, Halliwell B, Gajewski E, Dizdaroglu M. Copper-ion-dependent damage to the bases in DNA in the presence of hydrogen-peroxide. Biochem J. 1991;273:601-604.
  19. Keyer K, Imlay JA. Superoxide accelerates DNA damage by elevating free-iron levels. Proc Natl Acad Sci USA. 1996;93:13635-13640.
  20. Kasprzak KS. Oxidative DNA and protein damage in metal-induced toxicity and carcinogenesis. Free Radic Biol Med. 2002;32: 958-967.
  21. Gao YG, Sriram M, Wang AH. Crystallographic studies of metal-ion DNA interactions-different binding modes of cobalt(III), copper(II) and barium(II) to N-7 of guanines in Z-DNA and a drug-DNA complex. Nucleic Acid Res. 1993;21: 4093-4101.
  22. Lloyd DR, Phillips DH. Oxidative DNA damage mediated by copper(III), iron(II) and nickel(II) Fenton reactions. Mutation Res. 1999;424:23-36.
  23. Garcia J, Subias G, Cuartero V, Herrero-Martin J. On the correlation between the X-ray absorption chemical shift and the formal valence state in the mixed-valence manganites. J Synchrotron Rad. 2010;17:386-392.
  24. May CJ, Canavan HE, Castner DG. Quantitative X-ray Photoelectron Spectroscopy and Time-of-Flight Secondary Ion Mass Spectrometry Characterization of the Components in DNA. Anal Chem. 2004;76:1114.
  25. Peeling J, Hruska FE, McIntyre NS. ESCA spectra and molecular charge distributions for some pyrimidine and purine bases. Can J Chem. 1978; 56:1555.
  26. Furukawa M, Fujisawa H, Katano S, Ogawawara H, Kim Y, Komeda T, Nilsson A, Kawai M. Geometrical characterization of pyrimidine base molecules adsorbed on Cu(110) surfaces: XPS and NEXAFS studies. Surf Sci. 2003;532-535:261-266.
  27. Ptasinska S, Stypczynska A, Nixon T, Mason N J, Klyachko D V, Sanche L. X-ray induced damage in DNA monitored by X-ray photoelectron spectroscopy. J Chem Phys. 2008;129:065102.