Role of Temperature and Gibberellic Acid in Stimulating Embryo Elongation and Radicle Emergence of Hepatica asiatica Nakai Seeds

  • Chon, Young Shin (Department of Horticultural Science, Gyeongnam National University of Science and Technology) ;
  • Jeong, Kyeong Jin (Graduate School, Gyeongnam National University of Science and Technology) ;
  • Lee, Sang Woo (Department of Horticultural Science, Gyeongnam National University of Science and Technology) ;
  • Yun, Jae Gill (Department of Horticultural Science, Gyeongnam National University of Science and Technology)
  • Received : 2014.09.25
  • Accepted : 2015.01.29
  • Published : 2015.06.30


This research was performed to determine the temperature requirements for embryo growth and radicle emergence of Hepatica asiatica Nakai, a perennial herb native to Korea. Seed viability, embryo growth, and radicle emergence were monitored in seeds exposed to various temperatures (10, 15, $20^{\circ}C$ and $30{\rightarrow}15^{\circ}C$). Laboratory experiments at various temperatures revealed that (1) embryo elongation occurred effectively between 10 and $15^{\circ}C$; (2) radicle emergence occurred only at $15^{\circ}C$; (3) a warm stratification (2-8 weeks at $30^{\circ}C$) was not required for embryo elongation and radicle emergence, and led to inhibition of radicle emergence; (4) application of gibberellic acid ($GA_3$) promoted embryo growth, but not radicle emergence. These results suggested that H. asiatica seeds have two separate mechanisms to overcome dormancy, either by $GA_3$ (morphological dormancy) or temperature (physiological dormancy), and warm stratification is not involved in breaking radicle dormancy. These findings indicate that H. asiatica seeds have morphophysiological dormancy.


Supported by : Ministry for Food, Agriculture, Forestry and Fisheries (MIFAFF)


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