Acute Toxicity of Cadmium on Gene Expression Profiling of Fleshy Shrimp, Fenneropenaeus Chinensis Postlarvae Using a cDNA Microarray

Microarray 분석을 이용한 대하 (Fenneropenaeus chinensis) 유생의 카드뮴 단기 노출에 따른 유전자변화

  • Received : 2014.10.01
  • Accepted : 2015.05.02
  • Published : 2015.05.30


Microarray technology provides a unique tool for the determination of gene expression at the level of messenger RNA (mRNA). This study, the mRNA expression profiles provide insight into the mechanism of action of cadmium in Fleshy shrimp (Fenneropenaeus chinensis). The ability of genomic technologies was contributed decisively to development of new molecular biomarkers and to the determination of new possible gene targets. Also, it can be approach for monitoring of trace metal using oligo-chip microarray-based in potential model marine user level organisms. 15K oligo-chip for F. chinensis that include mostly unique sets of genes from cDNA sequences was developed. A total of 13,971 spots (1,181 mRNAs up- regulated and 996 down regulated) were identified to be significantly expressed on microarray by hierarchical clustering of genes after exposure to cadmium for different conditions (Cd24-5000 and Cd48-1000). Most of the changes of mRNA expression were observed at the long time and low concentration exposure of Cd48-1000. But, gene ontology analysis (GO annotation) were no significant different between experiments groups. It was observed that mRNA expression of main genes involved in metabolism, cell component, molecular binding and catalytic function. It was suggested that cadmium inhibited metabolism and growth of F. chinensis.


Microarray;Fenneropenaeus chinensis;Cadmium;Toxicity


  1. Buikema, A.L. Jr., Niederlehner, B.R., Cairns, J. Jr., 1982. Biological monitoring. Part IV. Toxicity testing. Water Res., 16, 239-262.
  2. Carvalho, C.E.V., Cavalcante, M.P.O, Gomes, M.P., Faria, V.V., Rezende, C.E., 2001. Distribuicao de Metais Pesados em Mexilhoes (Perna perna, L.) da Ilha de Santana, Macae, SE, Brasil. Ecotoxicol. Environ. Rest., 4, 1-5.
  3. Carvalho, C.E.V., Fariam, V.V., Cavalcante, P.O., Gomes, M.P., Rezende, C.E., 2000. Distribuicao de Metais Pesados em Peixes Costeiros Bentonicos da Regiao de Macae, R.J., Brasil. Ecotoxicol. Environ. Rest., 3, 12-16.
  4. Bambang, Y., Charmantier, G., Thuet, P., Trilles, J-P., 1994. Effect of cadmium on survival and osmoregulation of various developmental stages of the shrimp Penaeus japonicus. Marine Biology, 123, 443-450.
  5. Barbieri, E., Passos, E. A., Garcia, C.A.B., 2005. Use of metabolism to evaluate the sub-lethal toxicity of mercury on Farfantepaneus brasiliensis larvae (Latreille 1817, crustacean), J. Shellfish Research, 24, 1229-1234.[1229:UOMTET]2.0.CO;2
  6. Changcheng, Y., 1984. The prawn (Penaeus orientalis Kishinouye) in Pohai Sea and their fishery. In: Gulland, J.A., Rothschild., Rothschild, B.J. (Eds.), Penaeid shrimps-Their Biology and Management. Fishing News Books / Blackwell Scientific Publication, Oxford press. pp. 49-60.
  7. Cleveland, W. S. 1979. Robust locally weighted regression and smoothing scatterplots. J. American Statistical Association, 74, 829-836.
  8. Barbieri, E., 2009. Effects of zinc and cadmium on oxygen consumption and ammonium excretion in pink shrimp (Farfantepenaeus paulensis, Perez-Farfante, 1967, Crustacea). Ecotoxicology, 18, 312-318.
  9. Benjamin, P., Carols, B., 2011. A genomic and ecotoxicological perspective of DNA array studies in aquatic environmental risk assessment. Aquatic Toxicology, 105, 40-49.
  10. Eimers, R.D., Evans, R.D., Welbourn, P.M., 2001. Cadmium accumulation in the freshwater isopod Asellus racovitzai: the relative importance of solute and particulate sources at trace concentrations, Environ Pollut., 111, 247-258.
  11. Eysink, G.G.J., Padua, H.B., Bertoletti, S.A.E., Pereira, D.N., 1988a. Metais pesados no vale do Ribeirae em Iguape Ilha Comprida. R. Ambiente., 2, 6-13.
  12. Eysink, G.G.J., Padua, H.B., Martins, M.C., 1988b. Presenca de Mercurio no Ambiente. R. Ambiente., 2, 43-50.
  13. Finney, D.J., 2001. Probit Analysis. 2nd ed. Cambridge: Cambridge University Press
  14. Geard, N., Wiles, J., 2005. A gene network model for developing cell lineages. Artificial Life, 11, 249-67.
  15. Irizarry, R.A., Bolstad, B.M., Collin, F., Cope, L.M., Hobbs, B., Speed, T.P., 2003. Summaries of Affymetrix Gene Chip probe level data. Nucleic Acids Res., 31, e15.
  16. Ki, J.S, Raisuddin, S., Lee, K-W., Hwang, D.S., Han, J.H., Rhee, J-S., Kim, I-C., Park, H.G., Ryu, J-C., Lee, J.S., 2009. Gene expression profiling of copper-induced responses in the intertidal copepod Tigriopus japonicus using a 6K oligochip microarray. Aquatic Toxicology, 93,177-187.
  17. Kim, S.Y., Yoon, N.N., Ji, H.S., Han, S.M., Kwon, D.M., Lee, K.S., 2010. Characteristics of Ecotoxicity in Industrial Effluent using Daphnia magna. The Annual Report of Busan Metropolitan City Institute of Health & Environment, 20, 131-142.
  18. Kubo, I., 1949. Studies on the penaeids of Japanese and its adjacent water. J. Tokyo College Fish, 36, 393-398.
  19. Landrum, R.F., Robbins, J.A., 1990. Bioavailability of sediment associated contaminants to benthic invertebrates. In: Bando R, Giesy J and Muntau H (eds). Sediments chemistry and toxicity of in-place pollutants, Boston, Lewis Publishers, pp. 237-263.
  20. Lee, D.O.C., Wickins, J.F., 1992. Crustacean Farming. Blackwell Scientific Publications, Oxford press, pp.392.
  21. Lee, H.Y., Lee, S.H., 1997. Impacts of Large-scale reclamation on environment in Korea. The Korean Geographical Society, 32, 463-478.
  22. Leu, J-H., Chen, S-H., Wang, Y-B., Chen, Y.C., Su, S-Y., Lin, C.Y., Ho, J.M., Lo, C.F., 2011. A review of the major penaeid shrimp EST studies and the construction of a shrimp transcriptome data based on the ESTs from four penaeid shrimp. Marine Biotechnology, 13, 608-621.
  23. Litchfield, J.T. Jr., Wilcoxon, F., 1949. A simplified method of evaluating dose-effect experiments. J. Pharmacol Exp Ther., 96, 99-113
  24. Mance, G., 1987. Pollution Threats of Heavy Metals in Aquatic Environments. Elsevier Appied. London, UK. Science Publishers Ltd, pp. 230.
  25. Maroni, G., Lastowski-Perry, D., Otto, E., Watson, D., 1986. Effects of heavy metals on Drosophila larvae and a metallothionein cDNA, Environ Health Perspect., 65, 108-116.
  26. Ministry of Oceans and Fisheries. Statistics of Fisheries Production. www. [accessed 10 September 2013].
  27. Munshi, A.B., Su, Y.Q., Li, S.J., 1996. Acute toxicities of copper, cadmium and Cu: Cd mixture to larvae of the shrimp Penaeus Penicilliatus. China Journal of Oenology and Limnology, 14, 170-174.
  28. Papathanassion, E., 1983. Effects of cadmium and mercury ions on respiration and survival of the common prawn Palaemon serratus. (Pennant). Rev. Inst. Oceanogr. Med., 70, 21-35.
  29. Reynoldson, T.B., 1987. The role of environmental factors in the ecology of tubificid oligochaetes-an experimental study. Holarct Ecol., 10, 241-248.
  30. Schilstra, M.J., Bolouri, H., 2002. Modeling the Regulation of Gene Expression in Genetic Regulatory Networks. Biocomputation group, University of Hertfordshire.
  31. Sheridan, P.F., Brower, J.A., Powers, J.E., 1984. Ecological interactions between penaeid shrimp and bottom fish assemblages. In: Gulland, J.A., Rothschild., Rothschild, B.J. (Eds.) Penaeid shrimps-Their Biology and Management. Fishing News Books/Blackwell Scientific Publication, Oxford press, pp. 4235-4253.
  32. Soegianto, A., Charmantier-Daures, M., Trilles, J.P., Charmatier, G., 1999. Impact of Cadmium on the Structure of Gills and Epipodites of the Shrimp Penaeus japonicus (Crustacea: Decapoda). Aquatic Living Resources, 12, 57-70.
  33. Tusher, V.G., Tibshirani, R., Chu, G., 1998. Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci, USA. pp. 5116-5121.
  34. Vohradsky, J., 2001. Neural model of the genetic network. J. Biol. Chem., 276, 36168-73.
  35. Wu, J.P., Chen, H.C., 2004. Effects of cadmium and zinc on oxygen consumption, ammonium excretion, and osmoregulation of white shrimp (Litopenaeus vannamei). Chemosphere, 57, 1591-1598.
  36. 1952. Frias-Espericueta MG, Voltolina D, Osuna-Lopez JI. Acute Toxicity of Cadmium, Mercury, and Lead to White leg shrimp (Litopenaeus vannamei) Postlarvae. Bull. Environmental Contamination and Toxicolog,. 67, 580-586.


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