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Development of molecular biological techniques for the differentiation of medicinal plant species

약용작물의 기원 판별에 관한 분자생물학적 기술 개발 현황

  • Han, Eun-Heui (Dept. of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science & Technology) ;
  • Kim, Yun-Hee (Dept. of Biology Education, College of Education, IALS, Gyeongsang National University) ;
  • Lee, Shin-Woo (Dept. of Agronomy & Medicinal Plant Resources, Gyeongnam National University of Science & Technology)
  • 한은희 (경남과학기술대학교, 생명과학대학, 농학.한약자원학부) ;
  • 김윤희 (경상대학교, 사범대학, 생물교육과 (농업생명과학연구원)) ;
  • 이신우 (경남과학기술대학교, 생명과학대학, 농학.한약자원학부)
  • Received : 2015.03.05
  • Accepted : 2015.03.15
  • Published : 2015.03.31

Abstract

Medicinal plants resources are becoming important assets since their usages have been expanded to the development of functional foods for human health, more attractive cosmetics, and pharmaceutical industries. However, their phylogenetic origins and names are different from each country and quite often they are mixed each other resulting in the confusion for consumers. In particular, when they are very similar based on their morphological characteristics and distributed as dried roots, it is extremely difficult to differentiate their origins even by specialists. Recently, "DNA barcodes" have been extensively applied to identify their origin of medicinal plant species. In this review, we tried to overview the current research achievements for the development of suitable "DNA barcodes" regarding to the differentiation of medicinal plant species. Furthermore, more advanced techniques including amplification refractory mutation system (ARMS)-PCR, multiplex single base extension (MSBE), high-resolution melting (HRM) curve analyses are also discussed for their practical applications in the authentification of particular medicinal plant species.

Keywords

Acknowledgement

Supported by : 농림축산식품부

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