Molecular Phylogenetic Diversity and Spatial Distribution of Bacterial Communities in Cooling Stage during Swine Manure Composting

  • Guo, Yan (College of Life Science, Shangqiu Normal University) ;
  • Zhang, Jinliang (College of Life Science, Shangqiu Normal University) ;
  • Yan, Yongfeng (College of Life Science, Shangqiu Normal University) ;
  • Wu, Jian (Department of Basci Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University) ;
  • Zhu, Nengwu (The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters of Ministry of Education, College of Environmental Science and Engineering, South China University of Technology) ;
  • Deng, Changyan (Ministry of Agriculture Key Laboratory of Swine Breeding and Genetics, College of Animal Science and Technology, Huazhong Agricultural University)
  • Received : 2014.11.17
  • Accepted : 2015.01.22
  • Published : 2015.06.01


Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and subsequent sub-cloning and sequencing were used in this study to analyze the molecular phylogenetic diversity and spatial distribution of bacterial communities in different spatial locations during the cooling stage of composted swine manure. Total microbial DNA was extracted, and bacterial near full-length 16S rRNA genes were subsequently amplified, cloned, RFLP-screened, and sequenced. A total of 420 positive clones were classified by RFLP and near-full-length 16S rDNA sequences. Approximately 48 operational taxonomic units (OTUs) were found among 139 positive clones from the superstratum sample; 26 among 149 were from the middle-level sample and 35 among 132 were from the substrate sample. Thermobifida fusca was common in the superstratum layer of the pile. Some Bacillus spp. were remarkable in the middle-level layer, and Clostridium sp. was dominant in the substrate layer. Among 109 OTUs, 99 displayed homology with those in the GenBank database. Ten OTUs were not closely related to any known species. The superstratum sample had the highest microbial diversity, and different and distinct bacterial communities were detected in the three different layers. This study demonstrated the spatial characteristics of the microbial community distribution in the cooling stage of swine manure compost.


Bacterial Community;Composting;Spatial Distribution;Diversity;Restriction Fragment Length Polymorphism;Cooling Stage


Supported by : National Natural Science Foundation of China


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