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Aerobic Stability and Effects of Yeasts during Deterioration of Non-fermented and Fermented Total Mixed Ration with Different Moisture Levels

  • Hao, W. (College of Engineering, China Agricultural University) ;
  • Wang, H.L. (College of Engineering, China Agricultural University) ;
  • Ning, T.T. (College of Engineering, China Agricultural University) ;
  • Yang, F.Y. (College of Animal Science and Technology, China Agricultural University) ;
  • Xu, C.C. (College of Engineering, China Agricultural University)
  • Received : 2014.10.29
  • Accepted : 2015.01.19
  • Published : 2015.06.01

Abstract

The present experiment evaluated the influence of moisture level and anaerobic fermentation on aerobic stability of total mixed ration (TMR). The dynamic changes in chemical composition and microbial population that occur after air exposure were examined, and the species of yeast associated with the deterioration process were also identified in both non-fermented and fermented TMR to deepen the understanding of aerobic deterioration. The moisture levels of TMR in this experiment were adjusted to 400 g/kg (low moisture level, LML), 450 g/kg (medium moisture level, MML), and 500 g/kg (high moisture level, HML), and both non-fermented and 56-d-fermented TMR were subjected to air exposure to determine aerobic stability. Aerobic deterioration resulted in high losses of nutritional components and largely reduced dry matter digestibility. Non-fermented TMR deteriorated during 48 h of air exposure and the HML treatment was more aerobically unstable. On dry matter (DM) basis, yeast populations significantly increased from $10^7$ to $10^{10}cfu/g$ during air exposure, and Candida ethanolica was the predominant species during deterioration in non-fermented TMR. Fermented TMR exhibited considerable resistance to aerobic deterioration. Spoilage was only observed in the HML treatment and its yeast population increased dramatically to $10^9cfu/g$ DM when air exposure progressed to 30 d. Zygosaccharomyces bailii was the sole yeast species isolated when spoilage occurred. These results confirmed that non-fermented and fermented TMR with a HML are more prone to spoilage, and fermented TMR has considerable resistance to aerobic deterioration. Yeasts can trigger aerobic deterioration in both non-fermented and fermented TMR. C. ethanolica may be involved in the spoilage of non-fermented TMR and the vigorous growth of Z. bailii can initiate aerobic deterioration in fermented TMR.

Keywords

Total Mixed Ration;Fermentation;Moisture Level;Aerobic Stability;Yeast

Acknowledgement

Supported by : National Natural Science Foundation of China, National Scientific and Technological, Agro-scientific Research

References

  1. AOAC. 1990. Official Methods of Analysis. 15th ed. Association of Official Analytical Chemists, Arlington, VA, USA.
  2. Cao, Y., T. Takahashi, and K. Horiguchi. 2009. Effects of addition of food by-products on the fermentation quality of a total mixed ration with whole crop rice and its digestibility, preference, and rumen fermentation in sheep. Anim. Feed Sci. Technol. 151:1-11. https://doi.org/10.1016/j.anifeedsci.2008.10.010
  3. Carvalho, B., C. A vila, M. G. C. P. Miguel, J. C. Pinto, M. C. Santos, and R. F. Schwan. 2014. Aerobic stability of sugarcane silage inoculated with tropical strains of lactic acid bacteria. Grass Forage Sci. http://dx.doi/10.1111/gfs.12117 https://doi.org/10.1111/gfs.12117
  4. Dalmau, E., J. L. Montesinos, M. Lotti, and C. Casas. 2000. Effect of different carbon sources on lipase production by Candida rugosa. Enzyme Microb. Technol. 26:657-663. https://doi.org/10.1016/S0141-0229(00)00156-3
  5. Filya, I. 2003. The effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corn and sorghum silages. J. Dairy Sci. 86:3575-3581. https://doi.org/10.3168/jds.S0022-0302(03)73963-0
  6. Kimura, M. and T. Ohta. 1972. On the stochastic model for estimation of mutational distance between homologous proteins. J. Mol. Evol. 2:87-90. https://doi.org/10.1007/BF01653945
  7. Kurtzman, C. P. and C. J. Robnett. 1998. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 73:331-371. https://doi.org/10.1023/A:1001761008817
  8. Liu, Q. H., T. Shao, and J. G. Zhang. 2013. Determination of aerobic deterioration of corn stalk silage caused by aerobic bacteria. Anim. Feed Sci. Technol. 183:124-131. https://doi.org/10.1016/j.anifeedsci.2013.05.012
  9. Makimura, K., S. Y. Murayama, and H. Yamaguchi. 1994. Detection of a wide range of medically important fungi by the polymerase chain reaction. J. Med. Microbiol. 40:358-364. https://doi.org/10.1099/00222615-40-5-358
  10. McDonald, P., A. R. Henderson, and S. J. E. Heron. 1991. The Biochemistry of Silage. 2nd ed. Chalcombe Publ., Cambrian Prrinters, Ltd., Merlow, Bucks, Aberystwyth, Wales, UK.
  11. Muck, R. E., R. E. Pitt, and R. Y. Leibensperger. 1991. A model of aerobic fungal growth in silage: 1. Microbial characteristics. Grass Forage Sci. 46:283-299. https://doi.org/10.1111/j.1365-2494.1991.tb02234.x
  12. Nishino, N. and H. Hattori. 2007. Resistance to aerobic deterioration of total mixed ration silage inoculated with and without homofermentative or heterofermentative lactic acid bacteria. J. Sci. Food Agric. 87:2420-2426. https://doi.org/10.1002/jsfa.2911
  13. Nishino, N., H. Wada, M. Yoshida, and H. Shiota. 2004. Microbial counts, fermentation products, and aerobic stability of whole crop corn and a total mixed ration ensiled with and without inoculation of Lactobacillus casei or Lactobacillus buchneri. J. Dairy Sci. 87:2563-2570. https://doi.org/10.3168/jds.S0022-0302(04)73381-0
  14. Ohyama, Y., S. Masaki, and S. Hara. 1975. Factors influencing aerobic deterioration of silages and changes in chemical composition after opening silos. J. Sci. Food Agric. 26:1137-1147. https://doi.org/10.1002/jsfa.2740260811
  15. Saitou, N. and M. Nei. 1987. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425.
  16. Scaccabarozzi, L., C. Locatelli, G. Pisoni, G. Manarolla, A. Casula, V. Bronzo, and P. Moroni. 2011. Short communication: Epidemiology and genotyping of Candida rugosa strains responsible for persistent intramammary infections in dairy cows. J. Dairy Sci. 94:4574-4577. https://doi.org/10.3168/jds.2011-4294
  17. Schmidt, R. J. and L. Kung Jr.. 2010. The effects of Lactobacillus buchneri with or without a homolactic bacterium on the fermentation and aerobic stability of corn silages made at different locations. J. Dairy Sci. 93:1616-1624. https://doi.org/10.3168/jds.2009-2555
  18. Sousa, M. J., F. Rodrigues, M. Corte-Real, and C. Leao. 1998. Mechanisms underlying the transport and intracellular metabolism of acetic acid in the presence of glucose in the yeast Zygosaccharomyces bailii. Microbiology 144:665-670. https://doi.org/10.1099/00221287-144-3-665
  19. Spoelstra, S. F., M. G. Courtin, and J. A. C. Van Beers. 1988. Acetic acid bacteria can initiate aerobic deterioration of whole crop maize silage. J. Agric. Sci. 111:127-132. https://doi.org/10.1017/S0021859600082915
  20. Tabacco, E., F. Righi, A. Quarantelli, and G. Borreani. 2011. Dry matter and nutritional losses during aerobic deterioration of corn and sorghum silages as influenced by different lactic acid bacteria inocula. J. Dairy Sci. 94:1409-1419. https://doi.org/10.3168/jds.2010-3538
  21. Thompson, J. D., D. G. Higgins, and T. J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res. 22:4673-4680. https://doi.org/10.1093/nar/22.22.4673
  22. Wang, F. and N. Nishino. 2008a. Ensiling of soybean curd residue and wet brewers grains with or without other feeds as a total mixed ration. J. Dairy Sci. 91:2380-2387. https://doi.org/10.3168/jds.2007-0821
  23. Wang, F. J. and N. Nishino. 2008b. Resistance to aerobic deterioration of total mixed ration silage: Effect of ration formulation, air infiltration and storage period on fermentation characteristics and aerobic stability. J. Sci. Food Agric. 88:133-140. https://doi.org/10.1002/jsfa.3057
  24. Wang, C. and N. Nishino. 2013. Effects of storage temperature and ensiling period on fermentation products, aerobic stability and microbial communities of total mixed ration silage. J. Appl. Microbiol. 114:1687-1695. https://doi.org/10.1111/jam.12200
  25. Weinberg, Z. G., Y. Chen, D. Miron, Y. Raviv, E. Nahim, A. Bloch, E. Yosef, M. Nikbahat, and J. Miron. 2011. Preservation of total mixed rations for dairy cows in bales wrapped with polyethylene stretch film - A commercial scale experiment. Anim. Feed Sci. Technol. 164:125-129. https://doi.org/10.1016/j.anifeedsci.2010.11.016
  26. Woolford, M. K. 1990. The detrimental effects of air on silage. J. Appl. Microbiol. 68:101-116.
  27. Xu, C. C., Y. M. Cai, N. Moriya, and M. Ogawa. 2007a. Nutritive value for ruminants of green tea grounds as a replacement of brewers' grains in totally mixed ration silage. Anim. Feed Sci. Technol. 138:228-238. https://doi.org/10.1016/j.anifeedsci.2006.11.014
  28. Xu, C. C., Y. Cai, J. G. Zhang, and M. Ogawa. 2007b. Fermentation quality and nutritive value of a total mixed ration silage containing coffee grounds at ten or twenty percent of dry matter. J. Anim. Sci. 85:1024-1029. https://doi.org/10.2527/jas.2005-628
  29. Xu, C. C., Y. M. Cai, J. Zhang, M. Fukasawa, and N. Moriya. 2008. Ensiling and subsequent ruminal degradation characteristics of barley tea grounds treated with contrasting additives. Anim. Feed Sci. Technol. 141:368-374. https://doi.org/10.1016/j.anifeedsci.2007.05.032
  30. Xu, C., H. Wang, F. Yang, and Z. Yu. 2011. Effect of an inoculant and enzymes on fermentation quality and nutritive value of erect milk vetch (Astragalusadsurgens Pall.) silages. J. Anim. Feed Sci. 20:449-460. https://doi.org/10.22358/jafs/66199/2011

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