• Jin, Hee (Graduate School of Pharmaceutical Sciences, Ewha Womans University) ;
  • Jin, Yeung Bae (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Ju-Woon (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jae-Kyung (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Yun-Sil (Graduate School of Pharmaceutical Sciences, Ewha Womans University)
  • Received : 2014.11.28
  • Accepted : 2014.12.28
  • Published : 2015.03.31


We analyzed the differential effects of histopathology, apoptosis and expression of radiation response genes after chronic low dose rate (LDR) and acute high dose rate (HDR) radiation exposure in spleen, lung and liver of rats. Female 6-week-old Sprague-Dawley rats were used. For chronic low-dose whole body irradiation, rats were maintained for 14 days in a $^{60}Co$ gamma ray irradiated room and received a cumulative dose of 2 Gy or 5 Gy. Rats in the acute whole body exposure group were exposed to an equal dose of radiation delivered as a single pulse ($^{137}Cs$-gamma). At 24 hours after exposure, spleen, lung and liver tissues were extracted for histopathologic examination, western blotting and RT-PCR analysis. 1. The spleen showed the most dramatic differential response to acute and chronic exposure, with the induction of substantial tissue damage by HDR but not by LDR radiation. Effects of LDR radiation on the lung were only apparent at the higher dose (5 Gy), but not at lower dose (2 Gy). In the liver, HDR and LDR exposure induced a similar damage response at both doses. RT-PCR analysis identified cyclin G1 as a LDR-responsive gene in the spleen of rats exposed to 2 Gy and 5 Gy gamma radiation and in the lung of animals irradiated with 5 Gy. 2. The effects of LDR radiation differed among lung, liver, and spleen tissues. The spleen showed the greatest differential effect between HDR and LDR. The response to LDR radiation may involve expression of cyclin G1.


Supported by : National Research Foundation of Korea (NRF)


  1. Luckey TD. Physiological benefits from low levels of ionizing radiation. Health Phys. 1982;43(6):771-789.
  2. Yonezawa M, Takeda A, Misonoh J. Acquired radioresistance after low dose X-irradiation in mice. J Radiat Res. 1990;31(3):256-262.
  3. Yonezawa M, Misonoh J, Hosokawa Y. Two types of X-ray-induced radioresistance in mice: presence of 4 dose ranges with distinct biological effects. Mutat Res. 1996;358(2):237-243.
  4. Ducoff HS. Form of the increased longevity of Tribolium after X-irradiation. Exp Gerontol. 1975;10(3-4):189-193.
  5. Mine M, Okumura Y, Ichimaru M, Nakamura T, Kondo S. Apparently beneficial effect of low to intermediate doses of A-bomb radiation on human lifespan. Int J Radiat Biol. 1990;58(6):1035-1043.
  6. James SJ, Makinodan T. T cell potentiation in normal and autoimmune-prone mice after extended exposure to low doses of ionizing radiation and/or caloric restriction. Int J Radiat Biol Relat Stud Phys Chem Med. 1988;53(1):137-152.
  7. James SJ, Enger SM, Peterson WJ, Makinodan T. Immune potentiation after fractionated exposure to very low doses of ionizing radiation and/or caloric restriction in autoimmune-prone and normal C57Bl/6 mice. Clin Immunol Immunopathol. 1990;55(3):427-437.
  8. Matsubara J, Turcanu V, Poindron P, Ina Y. Immune effects of low-dose radiation: short-term induction of thymocyte apoptosis and long-term augmentation of T-cell-dependent immune responses. Radiat Res. 2000;153(3):332-338.[0332:IEOLDR]2.0.CO;2
  9. Kojima S, Ishida H, Takahashi M, Yamaoka K. Elevation of glutathione induced by low-dose gamma rays and its involvement in increased natural killer activity. Radiat Res. 2002;157(3):275-280.[0275:EOGIBL]2.0.CO;2
  10. Lee YJ, Ducoff HS. Radiation factors and their influence on induction of oxygen resistance. Radiat Res. 1989;117(1):158-162.
  11. Miyachi Y, Yamada T. Low-dose X-ray-induced depression of sexual behavior in mice. Behav Brain Res. 1994;65(1):113-115.
  12. Shen RN, Lu L, Kaiser HE, Broxmeyer HE. Murine AIDS cured by low dosage total body irradiation. Adv Exp Med Biol. 1997;407:451-458.
  13. Hashimoto S, Shirato H, Hosokawa M, Nishioka T, Kuramitsu Y, Matushita K, Kobayashi M, Miyasaka K. The suppression of metastases and the change in host immune response after low-dose total-body irradiation in tumor-bearing rats. Radiat Res. 1999;151(6):717-724.
  14. Takahashi M, Kojima S, Yamaoka K, Niki E. Prevention of type I diabetes by low-dose gamma irradiation in NOD mice. Radiat Res. 2000;154(6):680-685.[0680:POTIDB]2.0.CO;2
  15. Cardis E, Vrijheid M, Blettner M, Gilbert E, Hakama M, Hill C, Howe G, Kaldor J, Muirhead CR, Schubauer-Berigan M, Yoshimura T, Bermann F, Cowper G, Fix J, Hacker C, Heinmiller B, Marshall M, Thierry-Chef I, Utterback D, Ahn YO, Amoros E, Ashmore P, Auvinen A, Bae JM, Bernar J, Biau A, Combalot E, Deboodt P, Diez Sacristan A, Eklöf M, Engels H, Engholm G, Gulis G, Habib RR, Holan K, Hyvonen H, Kerekes A, Kurtinaitis J, Malker H, Martuzzi M, Mastauskas A, Monnet A, Moser M, Pearce MS, Richardson DB, Rodriguez- Artalejo F, Rogel A, Tardy H, Telle-Lamberton M, Turai I, Usel M, Veress K. The 15-Country Collaborative Study of Cancer Risk among Radiation Workers in the Nuclear Industry: estimates of radiation-related cancer risks. Radiat Res. 2007;167(4):396-416.
  16. Kadhim MA, Lorimore SA, Townsend KM, Goodhead DT, Buckle VJ, Wright EG. Radiation-induced genomic instability: delayed cytogenetic aberrations and apoptosis in primary human bone marrow cells. Int J Radiat Biol. 1995;67(3):287-293.
  17. Morgan WF, Day JP, Kaplan MI, McGhee EM, Limoli CL. Genomic instability induced by ionizing radiation. Radiat. Res. 1996;146(3):247-258.
  18. Suzuki K, Ojima M, Kodama S, Watanabe M. Radiation-induced DNA damage and delayed induced genomic instability. Oncogene. 2003;22(45):6988-6993.
  19. Kang CM, Park KP, Song JE, Jeoung DI, Cho CK, Kim TH, Bae S, Lee SJ, Lee YS. Possible biomarkers for ionizing radiation exposure in human peripheral blood lymphocytes. Radiat Res. 2003;159(3):312-319.[0312:PBFIRE]2.0.CO;2
  20. Lim YB, Pyun BJ, Lee HJ, Jeon SR, Jin YB, Lee YS. Proteomic identification of radiation response markers in mouse intestine and brain. Proteomics. 2011;11(7):1254-1263.
  21. Lee HJ, Lee M, Kang CM, Jeoung D, Bae S, Cho CK, Lee YS. Identification of possible candidate biomarkers for local or whole body radiation exposure in C57BL/6 mice. Int J Radiat Oncol Biol Phys. 2007;69(4):1272-1281.
  22. Ruka W, Taghian A, Gioioso D, Fletcher JA, Preffer F, Suit HD. Comparison between the in vitro intrinsic radiation sensitivity of human soft tissue sarcoma and breast cancer cell lines. J Surg Oncol. 1996;61(4):290-294.<290::AID-JSO13>3.0.CO;2-A
  23. Tapio S. Ionizing radiation effects on cells, organelles and tissues on proteome level. Adv Exp Med Biol. 2013;990:37-48.
  24. Lee WJ, Majumder ZR, Jeoung DI, Lee HJ, Kim SH, Bae S, Lee YS. Organ-specific gene expressions in C57BL/6 mice after exposure to low-dose radiation. Radiat Res. 2006;165(5):562-569.
  25. Cheng GH, Wu N, Jiang DF, Zhao HG, Zhang Q, Wang JF, Gong SL. Increased levels of p53 and PARP-1 in EL-4 cells probably related with the immune adaptive response induced by low dose ionizing radiation in vitro. Biomed Environ Sci. 2010;23(6):487-495.
  26. Ina Y, Tanooka H, Yamada T, Sakai K. Suppression of thymic lymphoma induction by life-long low-dose-rate irradiation accompanied by immune activation in C57BL/6 mice. Radiat Res. 2005;163(2):153-158.
  27. Yu HS, Song AQ, Lu YD, Qiu WS, Shen FZ. Effects of low-dose radiation on tumor growth, erythrocyte immune function and SOD activity in tumor-bearing mice. Chin Med J (Engl). 2004;117(7):1036-1039.
  28. Gong SL, Liu SC, Liu JX, Zhang YC, Liu SZ. Adaptive response of thymocyte apoptosis and cell cycle progression induced by low dose X-ray irradiation in mice. Biomed Environ Sci. 2000;13(3):180-188.
  29. Nomura T, Kinuta M, Hongyo T, Nakajima H, Hatanaka T. Programmed cell death in whole body and organ systems by low dose radiation. J. Radiat Res. 1992;33 Suppl:109-123.
  30. Mori N, van Wezel T, van der Valk M, Yamate J, Sakuma S, Okumoto M, Demant P. Genetics of susceptibility to radiation-induced apoptosis in colon: two loci on chromosomes 9 and 16. Mamm. Genome. 1998;9(5):377-380.
  31. Kimura SH, Nojima H. Cyclin G1 associates with MDM2 and regulates accumulation and degradation of p53 protein. Genes Cells. 2002;7(8):869-880.