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Single Oral Dose Toxicity Test of Blue Honeysuckle Concentrate in Mice

  • Kim, Hyung-Soo (Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University) ;
  • Park, Sang-In (Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University) ;
  • Choi, Seung-Hoon (Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University) ;
  • Song, Chang-Hyun (Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University) ;
  • Park, Soo-Jin (Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University) ;
  • Shin, Yong-Kook (Department of Natural Medicine Resources, Semyung University) ;
  • Han, Chang-Hyun (Department of Medical History & Literature Group, Korea Institute of Oriental Medicine) ;
  • Lee, Young Joon (The Medical Research Center for Globalization of Herbal Formulation, Daegu Haany University) ;
  • Ku, Sae-Kwang (Department of Anatomy and Histology, College of Korean Medicine, Daegu Haany University)
  • Received : 2014.12.17
  • Accepted : 2015.03.16
  • Published : 2015.03.31

Abstract

The objective of this study was to obtain single oral dose toxicity information for concentrated and lyophilized powder of blue honeysuckle (Lonicera caerulea L., Caprifoliaceae; BHcL) in female and male ICR mice to aid in the process of developing natural origin medicinal ingredients or foods following proximate analysis and phytochemical profile measurement. The proximate analysis revealed that BHcL had an energy value of 3.80 kcal/g and contained 0.93 g/g of carbohydrate, 0.41 g/g of sugar, 0.02 g/g of protein, and 0.20 mg/g of sodium. BHcL did not contain lipids, including saturated lipids, trans fats, or cholesterols. Further, BHcL contained 4.54% of betaine, 210.63 mg/g of total phenols, 159.30 mg/g of total flavonoids, and 133.57 mg/g of total anthocyanins. Following administration of a single oral BHcL treatment, there were no treatment-related mortalities, changes in body weight (bw) or organ weight, clinical signs, necropsy or histopathological findings up to 2,000 mg/kg bw, the limited dosage for rodents of both sexes. We concluded that BHcL is a practically non-toxic material in toxicity potency.

Acknowledgement

Grant : CHR(38)lt;신동의보감CHR(38)gt; 편찬 사업-역사문헌 증보와 현대한의지식 통합

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