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Lack of Influence of the ACE1 Gene I/D Polymorphism on the Formation and Growth of Benign Uterine Leiomyoma in Turkish Patients

  • Gultekin, Guldal Inal (Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University) ;
  • Yilmaz, Seda Gulec (Department of Molecular Medicine, Institute of Health Sciences, Faculty of Medicine, Yeditepe University) ;
  • Kahraman, Ozlem Timirci (Department of Molecular Medicine, Institute of Experimental Medicine, Istanbul University) ;
  • Atasoy, Hande (Department of Molecular Medicine, Institute of Health Sciences, Faculty of Medicine, Yeditepe University) ;
  • Dalan, A. Burak (Yeditepe University Hospital, Faculty of Medicine, Yeditepe University) ;
  • Attar, Rukset (Department of Gynecology, Faculty of Medicine, Yeditepe University) ;
  • Buyukoren, Ahmet (Department of Gynecology, Faculty of Medicine, Istanbul University) ;
  • Ucunoglu, Nazli (Department of Molecular Medicine, Institute of Health Sciences, Faculty of Medicine, Yeditepe University) ;
  • Isbir, Turgay (Department of Medical Biology, Faculty of Medicine, Yeditepe University)
  • Published : 2015.03.04

Abstract

Uterine leiomyomas (ULM), are benign tumors of the smooth muscle cells of the myometrium. They represent a common health problem and are estimated to be present in 30-70% of clinically reproductive women. Abnormal angiogenesis and vascular-related growth factors have been suggested to be associated with ULM growth. The angiotensin-I converting enzyme (ACE) is related with several tumors. The aim of this study was to identify possible correlation between ULM and the ACE I/D polymorphism, to evaluate whether the ACE I/D polymorphism could be a marker for early diagnosis and prognosis. ACE I/D was amplified with specific primer sets recognizing genomic DNA from ULM (n=72) and control (n=83) volunteers and amplicons were separated on agarose gels. The observed genotype frequencies were in agreement with Hardy-Weinberg equilibrium ($x^2=2.162$, p=0.339). There was no association between allele frequencies and study groups ($x^2=0.623$; p=0.430 for ACE I allele, $x^2=0.995$; p=0.339 for ACE D allele). In addition, there were no significant differences between ACE I/D polymorphism genotype frequencies and ULM range in size and number ($X^2=1.760;$ p=0.415 for fibroid size, $X^2=0.342;$ p=0.843 for fibroid number). We conclude that the ACE gene I/D polymorphism is not related with the size or number of ULM fibroids in Turkish women. Thus it cannot be regarded as an early diagnostic parameter nor as a risk estimate for ULM predisposition.

Keywords

Angiogenesis;benign cancer;uterine fibroid;uterine myoma;ACE insertion/deletion

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