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SRD5A2 Gene Polymorphisms and the Risk of Benign Prostatic Hyperplasia but not Prostate Cancer

  • Choubey, Vimal Kumar (Department of Urology, King George Medical University) ;
  • Sankhwar, Satya Narayan (Department of Urology, King George Medical University) ;
  • Carlus, S. Justin (Centre for Cellular and Molecular Biology) ;
  • Singh, Anand Narayan (Department of Urology, King George Medical University) ;
  • Dalela, Divakar (Department of Urology, King George Medical University) ;
  • Thangaraj, Kumarasamy (Centre for Cellular and Molecular Biology) ;
  • Rajender, Singh (Division of Endocrinology, Central Drug Research Institute)
  • Published : 2015.03.04

Abstract

Background: Testosterone, a primary androgen in males, is converted into its most active form, dihydrotestosterone (DHT), by $5{\alpha}$-reductase type 2 (encoded by the SRD5A2 gene) in the prostate. DHT is necessary for prostatic growth and has five times higher binding affinity than testosterone for androgen receptors. We hypothesized that polymorphic variations in the SRD5A2 gene may affect the risk of benign prostatic hyperplasia and prostate cancer. Materials and Methods: We analyzed SRD5A2 gene polymorphisms in 217 BPH patients, 192 PCa cases, and 171 controls. Genotyping was undertaken using direct DNA sequencing. Genotype data were compared between cases and controls using a Chi square statistical tool. Results: We found that the A49T locus was monomorphic with 'AA' genotype in all subjects. At V89L locus, the presence of 'VV' showed a marginally significant correlation with increased BPH risk (p=0.047). At the $(TA)_n$ locus, longer TA repeats were found to be protective against BPH (p=0.003). However, neither of these polymoprhisms correlated with the risk of PCa. Conclusions: We conclude that A49T is monomorphic in the study population, VV marginally correlates with BPH risk, and longer $(TA)_n$ repeats are protective against BPH. None of these polymorphisms affect the risk of PCa.

Keywords

$5{\alpha}$-reductase type 2;benign prostatic hyperplasia;genetic polymorphism;prostate cancer;SRD5A2

Acknowledgement

Supported by : Indian Council of Medical Research (ICMR)

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