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Short Low Concentration Cisplatin Treatment Leads to an Epithelial Mesenchymal Transition-like Response in DU145 Prostate Cancer Cells

  • Liu, Yi-Qing (Department of Clinical Laboratory, Shandong Provincial Hospital affiliated to Shandong University, Shandong University) ;
  • Zhang, Guo-An (Department of The Morphology Laboratory, Jining Medical University) ;
  • Zhang, Bing-Chang (Department of Clinical Laboratory, Shandong Provincial Hospital affiliated to Shandong University, Shandong University) ;
  • Wang, Yong (Department of Clinical Laboratory, Shandong Provincial Hospital affiliated to Shandong University, Shandong University) ;
  • Liu, Zheng (Department of Urology, Shandong Provincial Hospital affiliated to Shandong University, Shandong University) ;
  • Jiao, Yu-Lian (Department of Central Laboratory, Shandong Provincial Hospital affiliated to Shandong University, Shandong University) ;
  • Liu, Ning (Department of Information Technology, Jining Medical University) ;
  • Zhao, Yue-Ran (Department of Central Laboratory, Shandong Provincial Hospital affiliated to Shandong University, Shandong University)
  • Published : 2015.03.04

Abstract

Background: Prostate cancer is one of the main causes of cancer death, and drug resistance is the leading reason for therapy failure. However, how this occurs is largely unknown. We therrfore aimed to study the response of DU145 cells to cisplatin. Materials and Methods: Du145 prostate cancer cells were treated with a low dose of cisplatin for 24 h and cell viability and number were determined by MTT assay and trypan blue exclusion assay, respectively. The real time polymerase chain reaction (PCR) was used to assess responses to cisplatin treatment. Results: After 24h $2{\mu}g/ml$ treatment did not result in significant reduction in cell viability or number. However, it led to enhanced cancer cell invasiveness. E-cadherin mRNA was reduced, and vimentin, Snail, Slug, metalloproteinase 9 (MMP9) mRNA expression increased significantly, a feature of epithelial-mesenchymal transition (EMT). Conclusions: Short time low concentration cisplatin treatment leads to elevated invasiveness of DU145 cancer cells and this is possibly due to EMT.

Keywords

Epithelial-mesenchymal transition;prostate cancer;cisplatin;cancer invasiveness

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