KRT13, FAIM2 and CYP2W1 mRNA Expression in Oral Squamous Cell Carcinoma Patients with Risk Habits

  • Published : 2015.03.04


Background: Expression of KRT13, FAIM2 and CYP2W1 appears to be influenced by risk habits, thus exploring the associations of these genes in oral squamous cell cancer (OSCC) with risk habits, clinico-pathological parameters and patient survival may be beneficial in identifying relevant biomarkers with different oncogenic pathways. Materials and Methods: cDNAs from 41 OSCC samples with and without risk habits were included in this study. Quantitative real-time PCR was used to analyze KRT13, FAIM2 and CYP2W1 in OSCC. The housekeeping gene (GAPDH) was used as an endogenous control. Results: Of the 41 OSCC samples, KRT13 was down-regulated in 40 samples (97.6%), while FAIM2 and CYP2W1 were down-regulated in 61.0% and 48.8%, respectively. Overall, there were no associations between KRT13, FAIM2 and CYP2W1 expression with risk habits, selected socio-demographic and clinico-pathological parameters and patient survival. Conclusions: Although this study was unable to show significance, there were some tendencies in the associations of KRT13, FAIM2 and CYP2W1 expression in OSCC with selected clinic-pathological parameters and survival.


Supported by : MoE


  1. Aung P.P, Oue N, Mitani Y, et al (2006). Systematic research for gastric cancer-specific genes based on SAGE data: melanoma inhibitory and matrixmetalloproteinase-10 are novel prognostic factors in patients with gastric cancer. Oncogene, 25, 2546-57
  2. Bloor BK, Seddon SV, Morgan PR (2001). Gene expression of differentiation-specific keratin in oral epithelial dysplasia and squamous cell carcinoma. Oral Oncol, 37, 251-61
  3. Boender AJ, Rozen AJ, Adan RAH (2012). Nutritional state affects the expression of the obesity-associated genes Etv5, Faim2, Fto, and Negr1. Obesity, 20, 2420-5
  4. Carrilho C, Alberto M, Buane L, et al (2004). Keratin 8, 10, 13, and 17 are useful markers in the diagnosis of human cervix carcinomas. Human Pathol, 35, 546-51
  5. Cheong SC, Chadramouli GVR, Saleh A, et al (2009). Gene expression in human oral squamous cell carcinoma is influenced by risk factor exposure. Oral Oncol, 45, 712-9
  6. Edler D, Stenstedt K, Ohrling K, et al (2009). The expression of the novel CYP2W1 enzyme is an independent prognostic factor in colorectal cancer - A pilot study. Eur J Cancer, 45, 705-12
  7. Ferlay J, Shin HR, Bray F, et al (2010). Estimates of worldwide burden of cancer: GLOBOCAN 2008. Intern J Cancer, 127, 2893-917
  8. Fialka F, Gruber RM, Hitt R, et al (2008). CPA6, FMO2, LGI1, SIAT1 and TNC are differentially expressed in early- and late-stage oral squamous cell carcinoma--a pilot study. Oral Oncol, 44, 941-8
  9. Gomez A, Karlgren M, Edler D, et al (2007). Expression of CYP2W1 in colon tumour: regulation by gene methylation. Pharmacogenomics, 8, 1315-25
  10. Gupta B, Johnson NW (2014). Emerging and established global life-style risk factors for cancer of the upper aero-digestive tract. Asian Pac J Cancer Prev, 15, 5983-91.
  11. Karlgren M, Miura S, Ingelman-Sunberg M (2005). Novel extrahepatic cytochrome P450s. Toxicol Applied Pharmacol, 207, 57-61
  12. Karlgren M, Gomez A, Stark K, et al (2006). Tumor-specific expression of the novel cytochrome P450 enzyme, CYP2W1. Biochem Biophys Res Commun, 341, 451-8
  13. Krishna Rao SV, Mejia G, Roberts-Thomson K, et al (2013). Epidemiology of oral cancer in Asia in the past decade--an update (2000-2012). Asian Pac J Cancer Prev, 14, 5567-77
  14. Lallemant B, Evrard A, Combescure C, et al (2009). Clinical relevance of nine transcriptional molecular markers for the diagnosis of head and neck squamous cell carcinoma in tissue and saliva rinse. BMC Cancer, 9, 370
  15. Loyha K, Vatanasapt P, Promthet S, et al (2012). Risk factors for oral cancer in northeast Thailand. Asian Pac J Cancer Prev, 13, 5087-90
  16. Mikami T, Cheng J, Maruyama S, et al (2011). Emergence of keratin 17 vs. loss of keratin 13: Their reciprocal immunohistochemical profiles in oral carcinoma in situ. Oral Oncol, 47, 497-503
  17. Nishida CR, Lee M, De Montellano PR (2010). Efficient hypoxic activation of the anticanceragent AQ4N by CYPS1 and CYP2W1. Mol Pharmacol, 78, 497-502
  18. Petti S (2009). Lifestyle risk factors for oral cancer. Oral Oncol, 45, 340-50
  19. Reich A, Spering C, Gertz K, et al (2011). Fas/CD95 Regulatory Protein Faim2 is neuroprotective after transient Brain Ischemia. J Neurosci, 31, 225-33
  20. Scully C, Bagan JV (2009).Oral squamous cell carcinoma: overview of current understanding of aetiopathogenesis and clinical implications. Oral Diseases, 15, 388-99
  21. Somia NV, Schmitt MJ, Vetter DE, et al (1999). LFG: An antiapoptotic gene that provides protection from Fas-mediated cell death. Proc Nati Acad Sci U S A, 96, 12667-72
  22. Stenstedt K, Hallstorm M, Johansson I, et al (2012). The expression of CYP2W1: a prognostic marker in colon cancer. Anticancer Res, 32, 3869-74
  23. Warnakulasuriya S (2009). Global epidemiology of oral and oropharyngeal cancer. Oral Oncol, 45, 309-16
  24. Woolgar J.A, Scott J, Vaughan E.D, et al (1995). Survival, metastasis and recurrence of oral cancer in relation to pathological features. Ann R Coll Surg Engl, 77, 325-31

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