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Oral Concentrated Grape Juice Suppresses Expression of NF-kappa B, TNF-α and iNOS in Experimentally Induced Colorectal Carcinogenesis in Wistar Rats

  • de Lima Pazine Campanholo, Vanessa Maria (Oncology Group from Gastroenterology Division, Universidade Federal de Sao Paulo) ;
  • Silva, Roseane Mendes (Oncology Group from Gastroenterology Division, Universidade Federal de Sao Paulo) ;
  • Silva, Tiago Donizetti (Oncology Group from Gastroenterology Division, Universidade Federal de Sao Paulo) ;
  • Neto, Ricardo Artigiani (Oncology Group from Gastroenterology Division, Universidade Federal de Sao Paulo) ;
  • Paiotti, Ana Paula Ribeiro (Oncology Group from Gastroenterology Division, Universidade Federal de Sao Paulo) ;
  • Ribeiro, Daniel Araki (Oncology Group from Gastroenterology Division, Universidade Federal de Sao Paulo) ;
  • Forones, Nora Manoukian (Oncology Group from Gastroenterology Division, Universidade Federal de Sao Paulo)
  • Published : 2015.03.04

Abstract

The aim of this study was to evaluate the effects of grape juice on colon carcinogenesis induced by azoxymethane (AOM) and expression of NF-kB, iNOS and TNF-${\alpha}$. Methods: Forty male Wistar rats were divided into 7 groups: G1, control; G2, 15 mg/kg AOM; G3, 1% grape juice 2 weeks before AOM; G4, 2% grape juice 2 weeks before AOM; G5, 1% grape juice 4 weeks after AOM; G6, 2% grape juice 4 weeks after AOM; G7, 2% grape juice without AOM. Histological changes and aberrant crypt foci (ACF) were studied, while RNA expression of NF-kB, TNF- and iNOS was evaluated by qPCR. Results: The number of ACF was higher in G2, and G4 presented a smaller number of crypts per focus than G5 (p=0.009) and G6. Small ACF (1-3) were more frequent in G4 compared to G2, G5 and G6 (p=0.009, p=0.009 and p=0.041, respectively). RNA expression of NF-kB was lower in G3 and G4 compared to G2 (p=0.004 and p=0.002, respectively). A positive correlation was observed between TNF-${\alpha}$ and NF-kB gene expression (p=0.002). In conclusion, the administration of 2% grape juice before AOM reduced the crypt multiplicity, attenuating carcinogenesis. Lower expression of NF-kB was observed in animals exposed to grape juice for a longer period of time, regardless of concentration.

Keywords

Grape juice;NF-kappa B;TNF-${\alpha}$;iNOS;carcinogenesis

Acknowledgement

Supported by : Sao Paulo Research Foundation (FAPESP)

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