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Evaluation of Antitumor and Antioxidant Activity of Sargassum tenerrimum against Ehrlich Ascites Carcinoma in Mice

  • Patra, Satyajit (Division of Biochemistry and Genetics, American International Medical University) ;
  • Muthuraman, Meenakshi Sundaram (Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University) ;
  • Prabhu, A.T.J. Ram (Department of Biotechnology, Srimad Andavan Arts & Science College) ;
  • Priyadharshini, R. Ramya (Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University) ;
  • Parthiban, Sujitha (Department of Biotechnology, School of Chemical and Biotechnology, SASTRA University)
  • Published : 2015.03.04

Abstract

Context: In the last half century, discovering, developing and introducing of clinical agents from marine sources have seen great successes, with examples including the anti-cancer compound trabectedin. However, with increasing need for new anticancer drugs, further exploration for novel compounds from marine organism sources is strongly justified. Objective: The major aim of this study was to evaluate the antitumor and antioxidant potential of Sargassum tenerrimum J.Agardh (Sargassaceae) on Ehrlich ascites carcinoma (EAC) in Swiss albino mice. Materials and Methods: An ethanol extract of S. tenerrimum (EEST) from whole algae was used to evaluate cytotoxicity followed by in vivo assessment of toxicity, using biochemical parameters including hepatic and non-hepatic enzymes. Antioxidant properties were examined in animals bearing EAC treated with daily oral administration of 100-300 mg/kg extract suspension. Results: Antitumor effects of EEST in EAC bearing mice was observed with LD50 1815 mg/kg. Parameters like body weight, tumor volume, packed cell volume, tumor cell count, mean survival time and increase in life span in animals in the EAC bearing animals treated with EEST 300 mg/kg was comparable with control group. Significant differences were also seen with changes in total protein content, hepatic enzymes contents, MDA level, and free radical scavenging enzymes in untreated vs. EEST treated group animals. Conclusions: Evaluation of antioxidant enzymes and hepatic enzymes in the EAC animal model treated with EEST exhibited similar effects as the positive control drug 5-flurouracil. S. tenerrimum extracts contain effective antioxidants with significant antitumor activity.

Keywords

Algae ethanolic extract;cytotoxic activity;survival time;hepatic enzymes;lipid peroxidation

References

  1. Ahmed MI, Fayed ST, Hossein H, et al (1999). Lipid peroxidation and antioxidant status in human cervical carcinoma. Dis Markers, 15, 283-91. https://doi.org/10.1155/1999/963176
  2. Ali R, Mirza Z, Ashraf GM, et al (2012). New anticancer agents: recent developments in tumor therapy. Anticancer Res, 32, 2999-3005.
  3. Bodansky O (1973). Biochemical tests for cancer. CA Cancer J Clin, 23, 275-80. https://doi.org/10.3322/canjclin.23.5.275
  4. Brown TD, Burris HA, Havlin KA, et al (1991). New anticancer agents. Cancer Chemother Biol Response Modif, 12, 111-46.
  5. Burney IA, Furrukh M, Al-Moundhri MS (2014). What are our Options in the Fight against Breast Cancer? Sultan Qaboos Univ Med J, 14, 149-51.
  6. Calixto-Campos C, Zarpelon AC, Correa M, et al (2013). The Ehrlich tumor induces pain-like behavior in mice: a novel model of cancer pain for pathophysiological studies and pharmacological screening. Biomed Res Int, 2013, 624815.
  7. Chen X, Nie W, Yu G, et al (2012). Antitumor and immunomodulatory activity of polysaccharides from Sargassum fusiforme. Food Chem Toxicol, 50, 695-700. https://doi.org/10.1016/j.fct.2011.11.015
  8. Czeczot H, Scibior-Bentkowska D, Skrzycki M, et al (2010). [Lipid peroxidation level in gastrointestinal tract tumors]. Pol Merkur Lekarski, 29, 309-14.
  9. Desbois AP (2014). How might we increase success in marinebased drug discovery? Expert Opin Drug Discov, 1-6.
  10. Dolai N, Karmakar I, Suresh Kumar RB, et al (2012). Evaluation of antitumor activity and in vivo antioxidant status of Anthocephalus cadamba on Ehrlich ascites carcinoma treated mice. J Ethnopharmacol, 142, 865-70. https://doi.org/10.1016/j.jep.2012.05.050
  11. Dolma S, Lessnick SL, Hahn WC, et al (2003). Identification of genotype-selective antitumor agents using synthetic lethal chemical screening in engineered human tumor cells. Cancer Cell, 3, 285-96. https://doi.org/10.1016/S1535-6108(03)00050-3
  12. El-Shemy HA, Aboul-Soud MA, Nassr-Allah AA, et al (2010). Antitumor properties and modulation of antioxidant enzymes' activity by Aloe vera leaf active principles isolated via supercritical carbon dioxide extraction. Curr Med Chem, 17, 129-38. https://doi.org/10.2174/092986710790112620
  13. Feng Q, Kumagai T, Torii Y, et al (2001). Anticarcinogenic antioxidants as inhibitors against intracellular oxidative stress. Free Radic Res, 35, 779-88. https://doi.org/10.1080/10715760100301281
  14. Fini MA, Elias A, Johnson RJ, et al (2012). Contribution of uric acid to cancer risk, recurrence, and mortality. Clin Transl Med, 1, 16. https://doi.org/10.1186/2001-1326-1-16
  15. Gothoskar SV, Ranadive KJ (1971). Anticancer screening of SAN-AB: an extract of marking nut, Semecarpus anacardium. Indian J Exp Biol, 9, 372-5.
  16. Goto T, Tomizawa N, Kobayashi E, et al (2004). A comparative pharmacology study between the intracolonic and oral routes of 5-FU administration in a colon cancer-bearing Yoshida sarcoma rat model. J Pharmacol Sci, 95, 163-73. https://doi.org/10.1254/jphs.FP0040139
  17. Gupta M, Mazumder UK, Kumar RS, et al (2004). Antitumor activity and antioxidant status of Caesalpinia bonducella against Ehrlich ascites carcinoma in Swiss albino mice. J Pharmacol Sci, 94, 177-84. https://doi.org/10.1254/jphs.94.177
  18. Haldar PK, Kar B, Bala A, et al (2010). Antitumor activity of Sansevieria roxburghiana rhizome against Ehrlich ascites carcinoma in mice. Pharm Biol, 48, 1337-43. https://doi.org/10.3109/13880201003792592
  19. Jung H, Choi SC (1994). Sequential method of estimating the LD50 using a modified up-and-down rule. J Biopharm Stat, 4, 19-30. https://doi.org/10.1080/10543409408835069
  20. Khan SS, Nazim K, Mehmood KT, et al (2012). Phytochemical and antibacteria screening of crude extract of Sargassum tenerrimum J. Agardh against potential human pathogens. FUUAST Journal of Biology, 2, 65-8.
  21. Khanavi M, Nabavi M, Sadati N, et al (2010). Cytotoxic activity of some marine brown algae against cancer cell lines. Biol Res, 43, 31-7.
  22. Knopp KL, Nisenbaum ES, Arneric SP (2011). Evolving cancer pain treatments: rational approaches to improve the quality of life for cancer patients. Curr Pharm Biotechnol, 12, 1627-43. https://doi.org/10.2174/138920111798357267
  23. Kumar MS, Unnikrishnan MK, Patra S, et al (2003). Naringin and naringenin inhibit nitrite-induced methemoglobin formation. Pharmazie, 58, 564-6.
  24. Lee KH (1999). Anticancer drug design based on plant-derived natural products. J Biomed Sci, 6, 236-50.
  25. Lengyel Z, Boer K, Halaszlaki C, et al (2013). [Diabetes in patients with malignant tumors]. Magy Onkol, 57, 177-81.
  26. Li JJ, Oberley LW (1997). Overexpression of manganesecontaining superoxide dismutase confers resistance to the cytotoxicity of tumor necrosis factor alpha and/or hyperthermia. Cancer Res, 57, 1991-8.
  27. Lu C, Lee JJ, Komaki R, et al (2010). Chemoradiotherapy with or without AE-941 in stage III non-small cell lung cancer: a randomized phase III trial. J Natl Cancer Inst, 102, 859-65. https://doi.org/10.1093/jnci/djq179
  28. Miranda JJ, Kinra S, Casas JP, et al (2008). Non-communicable diseases in low- and middle-income countries: context, determinants and health policy. Trop Med Int Health, 13, 1225-34. https://doi.org/10.1111/j.1365-3156.2008.02116.x
  29. Misra HP, Fridovich I (1972). The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem, 247, 3170-5.
  30. Mohun AF, Cook IJ (1957). Simple methods for measuring serum levels of the glutamic-oxalacetic and glutamic-pyruvic transaminases in routine laboratories. J Clin Pathol, 10, 394-9. https://doi.org/10.1136/jcp.10.4.394
  31. Moron MS, Depierre JW, Mannervik B (1979). Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim Biophys Acta, 582, 67-78. https://doi.org/10.1016/0304-4165(79)90289-7
  32. Ohkawa H, Ohishi N, Yagi K (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95, 351-8. https://doi.org/10.1016/0003-2697(79)90738-3
  33. Ozkan A, Fiskin K (2004). Epirubicin HCl toxicity in humanliver derived hepatoma G2 cells. Pol J Pharmacol, 56, 435-44.
  34. Patra S, Muthuraman MS (2013). Gracilaria edulis extract induces apoptosis and inhibits tumor in Ehrlich ascites tumor cells in vivo. BMC Complement Altern Med, 13, 331. https://doi.org/10.1186/1472-6882-13-331
  35. Patra S, Muthuraman MS (2013). Gracilaria edulis extract induces apoptosis and inhibits tumor in Ehrlich ascites tumor cells in vivo. BMC Complement Altern Med, 13, 331. https://doi.org/10.1186/1472-6882-13-331
  36. Riely GJ, Gadgeel S, Rothman I, et al (2007). A phase 2 study of TZT-1027, administered weekly to patients with advanced non-small cell lung cancer following treatment with platinum-based chemotherapy. Lung Cancer, 55, 181-5. https://doi.org/10.1016/j.lungcan.2006.10.002
  37. Rudin CM, Yang Z, Schumaker LM, et al (2003). Inhibition of glutathione synthesis reverses Bcl-2-mediated cisplatin resistance. Cancer Res, 63, 312-8.
  38. Salem FS, Badr MO, Neamat-Allah AN (2011). Biochemical and pathological studies on the effects of levamisole and chlorambucil on Ehrlich ascites carcinoma-bearing mice. Vet Ital, 47, 89-95.
  39. Schwendel A, Siems WG, Grune T, et al (1994). Transitions of hepatic purine metabolism of Ehrlich ascites tumor bearing mice in different phases of tumor growth. Biochem Mol Biol Int, 34, 457-63.
  40. Seven A, Civelek S, Inci E, et al (1999). Evaluation of oxidative stress parameters in blood of patients with laryngeal carcinoma. Clin Biochem, 32, 369-73. https://doi.org/10.1016/S0009-9120(99)00022-3
  41. Sharma OP 1986. Textbook of Algae, New Delhi, Tata McGraw- Hill Education.
  42. Simmons TL, Andrianasolo E, McPhail K, et al (2005). Marine natural products as anticancer drugs. Mol Cancer Ther, 4, 333-42.
  43. Sinclair AJ, Barnett AH, Lunec J (1990). Free radicals and antioxidant systems in health and disease. Br J Hosp Med, 43, 334-44.
  44. Singaravelu G, Arockiamary JS, Kumar VG, et al (2007). A novel extracellular synthesis of monodisperse gold nanoparticles using marine alga, Sargassum wightii Greville. Colloids Surf B Biointerfaces, 57, 97-101. https://doi.org/10.1016/j.colsurfb.2007.01.010
  45. Sinha AK (1972). Colorimetric assay of catalase. Anal Biochem, 47, 389-94. https://doi.org/10.1016/0003-2697(72)90132-7
  46. Sinha AK (1972). Colorimetric assay of catalase. Anal Biochem, 47, 389-94. https://doi.org/10.1016/0003-2697(72)90132-7
  47. Sur P, Ganguly DK (1994). Tea plant root extract (TRE) as an antineoplastic agent. Planta Med, 60, 106-9. https://doi.org/10.1055/s-2006-959427
  48. Szabados G, Tretter L, Horvath I (1989). Lipid peroxidation in liver and Ehrlich ascites cell mitochondria. Free Radic Res Commun, 7, 161-70. https://doi.org/10.3109/10715768909087938
  49. Tohme R, Darwiche N, Gali-Muhtasib H (2011). A journey under the sea: the quest for marine anti-cancer alkaloids. Molecules, 16, 9665-96. https://doi.org/10.3390/molecules16119665
  50. Weber G (1983). Enzymes of purine metabolism in cancer. Clin Biochem, 16, 57-63. https://doi.org/10.1016/S0009-9120(83)94432-6
  51. Ye H, Wang K, Zhou C, et al (2008). Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum. Food Chemistry, 111, 428-32. https://doi.org/10.1016/j.foodchem.2008.04.012
  52. Yende SR, Harle UN, Chaugule BB (2014). Therapeutic potential and health benefits of species. Pharmacogn Rev, 8, 1-7. https://doi.org/10.4103/0973-7847.125514
  53. Zandi K, Ahmadzadeh S, Tajbakhsh S, et al (2010). Anticancer activity of Sargassum oligocystum water extract against human cancer cell lines. Eur Rev Med Pharmacol Sci, 14, 669-73.

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