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Overexpression of Tbx3 Predicts Poor Prognosis of Patients with Resectable Pancreatic Carcinoma

  • Wang, Hong-Cheng (Department of General Surgery, Sixth People's Hospital Affiliated Shanghai Jiao Tong University) ;
  • Meng, Qing-Cai (Department of General Surgery, Sixth People's Hospital Affiliated Shanghai Jiao Tong University) ;
  • Shan, Ze-Zhi (Department of General Surgery, Sixth People's Hospital Affiliated Shanghai Jiao Tong University) ;
  • Yuan, Zhou (Department of General Surgery, Sixth People's Hospital Affiliated Shanghai Jiao Tong University) ;
  • Huang, Xin-Yu (Department of General Surgery, Sixth People's Hospital Affiliated Shanghai Jiao Tong University)
  • Published : 2015.03.09

Abstract

Background: To determine the expressions of Tbx3, a member of subgroup belonging to T-box family, and its prognostic value in pancreatic carcinoma. Materials and Methods: We determined the expression levels of Tbx3 on both mRNA and protein levels in 30 pairs of fresh tumor tissues and paratumor tissues by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting, respectively. In addition, protein level of Tbx3 were identified using immunochemistry in 80 pairs of paraffin-embedded specimen. The correlations between Tbx3 expression and various clinicopathological parameters as well as overall survival were evaluated. Results: Tbx3 mRNA and protein levels in tumor tissues were significantly higher than in the paratumor tissues by qRT-PCR ($0.05{\pm}0.007$ vs. $0.087{\pm}0.001$, p<0.001) and western blotting ($1.134{\pm}0.043$ vs. $0.287{\pm}0.017$, p<0.001). The statistical analysis based on immunohistochemical evaluation suggested that Tbx3 aberrant expression was significantly associated with several conventional clinicopathological variables, such as gender, age, tumor position, preoperative CA19-9 level, pathological T staging and N staging. Univariate and multivariate analyses revealed that Tbx3 expression was an independent prognostic factor for overall survival (<0.001). Conclusions: Our results suggest that overexpression of Tbx3 is associated with poor prognosis of pancreatic cancer patients. However, additional clinical trials are needed to accurately validate this observation.

Keywords

Tbx3;pancreatic carcinoma;prognosis

Acknowledgement

Supported by : Science and Technology Commission of Shanghai Municipality

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