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Isolation and characterization analysis of the halophilic archaea isolated from solar saltern, Gomso

곰소 염전에서 분리한 호염성 고세균의 특성 분석

  • Koh, Hyeon-Woo (Department of Biology, Jeju National University) ;
  • Kim, So-Jeong (Department of Microbiology, Chungbuk National University) ;
  • Rhee, Sung-Keun (Department of Microbiology, Chungbuk National University) ;
  • Park, Soo-Je (Department of Biology, Jeju National University)
  • Received : 2015.08.26
  • Accepted : 2015.10.02
  • Published : 2015.12.31

Abstract

Most of halophilic archaea are found in the various hypersaline environments including solar saltern, salt lake with very high salt concentration. The present study is about isolation and characterization of halphilic archaea from Gomso solar saltern known as a representative high salt environment in Korea. In order to isolate the halophilic archaea, we prepared and used high salt medium. Finally, total 7 strains obtained were tentatively identified based on comparative similarity analysis for 16S rRNA gene sequence and physiological traits. All halophilic archaea belonged to Haloruburm, Halogeometriucm, Halobacterium, and Haloarcula genera. These isolates were all Gram-staining negative, and growth was not observed using nitrate as an alternative electron acceptor under anaerobic conditions. In addition, all isolates required about 12-30% (w/v, NaCl) salt. This case study might provide basic information on microbial isolation technologies and related research in halophilic microorganisms from domestic halophilic environments, and contribute to obtaining useful indigenous halophilic archaea in a variety of extreme environmental conditions.

Keywords

Gomso solar saltern;halophilic archaea

Acknowledgement

Supported by : 국립생물자원관, 충북대학교, 한국연구재단

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