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Isolation and identification of protease-producing bacteria from the intertidal zone in Jeju Island, Korea

제주 조간대로부터 단백질 가수분해효소를 생산하는 세균의 분리 및 동정

  • Moon, Young-Gun (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Life Sciences, Jeju National University) ;
  • Dharaneedharan, Subramanian (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Life Sciences, Jeju National University) ;
  • Kim, Dong-Hwi (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Life Sciences, Jeju National University) ;
  • Park, So-Hyun (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Life Sciences, Jeju National University) ;
  • Heo, Moon Soo (Marine Applied Microbes and Aquatic Organism Disease Control Lab, Department of Aquatic Biomedical Sciences, School of Marine Life Sciences, Jeju National University)
  • 문영건 (제주대학교 해양생명과학대학 수산의생명과학부) ;
  • 수브라마니안 다라니다란 (제주대학교 해양생명과학대학 수산의생명과학부) ;
  • 김동휘 (제주대학교 해양생명과학대학 수산의생명과학부) ;
  • 박소현 (제주대학교 해양생명과학대학 수산의생명과학부) ;
  • 허문수 (제주대학교 해양생명과학대학 수산의생명과학부)
  • Received : 2015.11.04
  • Accepted : 2015.12.24
  • Published : 2015.12.31

Abstract

Eleven protease-producing bacteria were isolated from the organisms' external shells and the inorganic materials collected from intertidal zone of Jeju Island, Republic of Korea. The samples were diluted serially, inoculated on Zobell agar plates with 1% skim milk and incubated at $20^{\circ}C$. Clear zone forming colonies were selected as protease-producing bacteria and each strain was identified based on the phylogenetic analysis with their 16S rDNA sequences. Strains JJM125, JJM129, YG47 and YG49 belong to the marine bacterial genus Pseudoalteromonas; strain JJM122 belong to the genus Microbulbifer; strains YG51, YG52, YG62 and YG63 belong to the genus Vibrio; and strain YG65 belong to genus Bacillus. Hence, the present study suggests that these protease producing bacteria could be further used to develop new varieties of protease with various biotechnological applications.

Keywords

bacteria;intertidal zone;Jeju Island;protease

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

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