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Growth kinetics and chlorine resistance of heterotrophic bacteria isolated from young biofilms formed on a model drinking water distribution system

모델 상수관망에 형성된 초기 생물막에서 분리한 종속영양세균의 생장 동역학 및 염소 내성

  • Park, Se-Keun (Department of Environmental Science & Biotechnology, Hallym University) ;
  • Kim, Yeong-Kwan (Department of Environmental Engineering, Kangwon National University) ;
  • Oh, Young-Sook (Department of Environmental Engineering and Energy, Myongji University) ;
  • Choi, Sung-Chan (Department of Environmental Science & Biotechnology, Hallym University)
  • 박세근 (한림대학교 환경생명공학과) ;
  • 김영관 (강원대학교 환경공학과) ;
  • 오영숙 (명지대학교 환경에너지공학과) ;
  • 최성찬 (한림대학교 환경생명공학과)
  • Received : 2015.10.26
  • Accepted : 2015.11.16
  • Published : 2015.12.31

Abstract

The present work quantified the growth of young biofilm in a model distribution system that was fed with chlorinated drinking water at a hydraulic retention time of 2 h. Bacterial biofilms grew on the surface of polyvinyl chloride (PVC) slides at a specific growth rate of $0.14{\pm}0.09day^{-1}$ for total bacteria and $0.16{\pm}0.08day^{-1}$ for heterotrophic bacteria, reaching $3.1{\times}10^4cells/cm^2$ and $6.6{\times}10^3CFU/cm^2$ after 10 days, respectively. The specific growth rates of biofilm-forming bacteria were found to be much higher than those of bulk-phase bacteria, suggesting that biofilm bacteria account for a major part of the bacterial production in this model system. Biofilm isolates exhibited characteristic kinetic properties, as determined by ${\mu}_{max}$ and $K_S$ values using the Monod model, in a defined growth medium containing various amounts of acetate. The lowest ${\mu}_{max}$ value was observed in bacterial species belonging to the genus Methylobacterium, and their slow growth seemed to confer high resistance to chlorine treatment (0.5 mg/L for 10 min). $K_S$ values (inversely related to substrate affinity) of Sphingomonas were two orders of magnitude lower for acetate carbon than those of other isolates. The Sphingomonas isolates may have obligate-oligotrophic characteristics, since the lower $K_S$ values allow them to thrive under nutrient-deficient conditions. These results provide a better understanding and control of multi-species bacterial biofilms that develop within days in a drinking water distribution system.

Keywords

Methylobacterium;Sphingomonas;biofilm;chlorine resistance;drinking water;growth kinetics

Acknowledgement

Grant : Projects for Developing Eco-Innovation Technologies

Supported by : Korea Ministry of Environment, Hallym University

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