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Defensive roles of Sdu1, a PPPDE superfamily member with ubiquitin C-terminal hydrolase activity, against thermal stress in Schizosaccharomyces pombe

카르복시 말단 유비퀴틴 가수분해 효소 활성 보유 PPPDE superfamily member인 Schizosaccharomyces pombe Sdu1의 열 스트레스에 대한 방어적 역할

  • Han, Hee (Department of Biochemistry, Kangwon National University) ;
  • Heo, Tae Young (Department of Biochemistry, Kangwon National University) ;
  • Ryu, In Wang (Department of Biological Sciences, Kangwon National University) ;
  • Kim, Kyunghoon (Department of Biological Sciences, Kangwon National University) ;
  • Lim, Chang-Jin (Department of Biochemistry, Kangwon National University)
  • 한희 (강원대학교 자연과학대학 생화학과) ;
  • 허태영 (강원대학교 자연과학대학 생화학과) ;
  • 류인왕 (강원대학교 자연과학대학 생명과학과) ;
  • 김경훈 (강원대학교 자연과학대학 생명과학과) ;
  • 임창진 (강원대학교 자연과학대학 생화학과)
  • Received : 2015.08.31
  • Accepted : 2015.11.04
  • Published : 2015.12.31

Abstract

The $sdu1^+$ gene encodes Sdu1, a PPPDE superfamily member of deubiquitinating enzymes (DUBs) in Schizosaccharomyces pombe. Sdu1 was previously shown to contain an actual ubiquitin C-terminal hydrolase (UCH) activity using the recombinant plasmid pYSTP which harbors the $sdu1^+$ gene. This work was designed to assess a thermotolerant role of Sdu1 against high incubation temperatures. In the temperature-shift experiments, the S. pombe cells harboring pYSTP grew much better after the shifts to $37^{\circ}C$ and $42^{\circ}C$, when compared with the vector control cells. After being shifted to $37^{\circ}C$ and $42^{\circ}C$ for 6 h, the S. pombe cells harboring pYSTP contained lower reactive oxygen species (ROS) levels, compared with the vector control cells. The nitric oxide (NO) levels of the S. pombe cells harboring pYSTP were slightly lower than those of the vector control cells in the absence or presence of the temperature shifting. The total glutathione (GSH) levels of the S. pombe cells harboring pYSTP were significantly higher than those of the vector control cells. Total superoxide dismutase (SOD) and GSH peroxidase activities were also higher in the S. pombe cells harboring pYSTP after the temperature shifts than in the vector control cells. In brief, the S. pombe Sdu1 plays a thermotolerant role against high incubation temperature through the down-regulation of ROS and NO and the up-regulation of total GSH content, total SOD and GSH peroxidase activities.

Keywords

Schizosaccharomyces pombe;glutathione;reactive oxygen species;Sdu1;thermal stress

Acknowledgement

Supported by : Kangwon National University

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