DOI QR코드

DOI QR Code

Effects of 60-Hz Time-Varying Electric Fields on DNA Damage and Cell Viability Support Negligible Genotoxicity of the Electric Fields

  • Yoon, Yeo Jun (Department of Biochemistry, College of Life Science & Biotechnology, Yonsei University) ;
  • Li, Gen (Department of Biochemistry, College of Life Science & Biotechnology, Yonsei University) ;
  • Kim, Gyoo Cheon (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Lee, Hae June (Department of Electrical Engineering, Pusan National University) ;
  • Song, Kiwon (Department of Biochemistry, College of Life Science & Biotechnology, Yonsei University)
  • Received : 2015.05.04
  • Accepted : 2015.07.01
  • Published : 2015.07.31

Abstract

The effect of a 60 Hz time-varying electric field was studied using a facing-electrode device (FED) and a coplanar-electrode device (CED) for further investigation of the genotoxicity of 60 Hz time-varying magnetic field (MF) from preceding research. Neither a single 30-minute exposure to the CED or to the FED had any obvious biological effects such as DNA double strand break (DSB) and apoptosis in cancerous SCC25, and HeLa cells, normal primary fibroblast IMR90 cells, while exposures of 60 Hz time-varying MF led to DNA damage with induced electric fields much smaller than those used in this experiment. Nor did repetitive exposures of three days or a continuous exposure of up to 144 hours with the CED induce any DNA damage or apoptosis in either HeLa or IMR90 cells. These results imply that the solitary electric field produced by time-varying MF is not a major cause of DSBs or apoptosis in cancer or normal cells.

References

  1. International Agency for Research on Cancer by the Secretariat of the World Health Organization, "IARC monographs on the evaluation of carcinogenic risks to humans," 2002; http://monographs.iarc.fr/ENG/Monographs/vol80/mono80.pdf.
  2. International Commission on Non-Ionizing Radiation Protection, "ICNIRP guidelines for limited exposure to time-varying electric and magnetic field (1 Hz-100 kHz)," 2010; http://www.icnirp.org/cms/upload/publications/ICNIRPLFgdl.pdf.
  3. C. Polk and E. Postow, Handbook of Biological Effects of Electromagnetic Fields, Boca Raton, FL: CRC Press, 1995.
  4. E. D. Kirson, Z. Gurvich, R. Schneiderman, E. Dekel, A. Itzhaki, Y. Wasserman, R. Schatzberger and Y. Palti, "Disruption of cancer cell replication by alternating electric fields," Cancer Research, vol. 64, no. 9, pp. 3288-3295, 2004. https://doi.org/10.1158/0008-5472.CAN-04-0083
  5. E. D. Kirson, V. Dbaly, F. Tovarys, J. Vymazal, J. F. Soustiel, A. Itzhaki, D. Mordechovich, S. SteinbergShapira, Z. Gurvich, R. Schneiderman, Y. Wasserman, M. Salzberg, B. Ryffel, D. Goldsher, E. Dekel, and Y. Palti, "Alternating electric fields arrest cell proliferation in animal tumor models and human brain tumors," Proceedings of the National Academy of Science of the United States of America, vol. 104, no. 24, pp. 10152-10157, 2007. https://doi.org/10.1073/pnas.0702916104
  6. J. Kim, C. S. Ha, H. J. Lee, and K. Song, "Repetitive exposure to a 60-Hz time-varying magnetic field induces DNA double-strand breaks and apoptosis in human cells," Biochemical and Biophysics Research Communications, vol. 400, no. 4, pp. 739-744, 2010. https://doi.org/10.1016/j.bbrc.2010.08.140
  7. J. Kim, Y. Yoon, S. Yun, G. S. Park, H. J. Lee, and K. Song, "Time-varying magnetic fields of 60 Hz at 7 mT induce DNA double-strand breaks and activate DNA damage checkpoints without apoptosis," Bioelectromagnetics, vol. 33, no. 5, pp. 383-393, 2012. https://doi.org/10.1002/bem.21697
  8. D. T. Vistica, P. Skehan, D. Scudiero, A. Monks, A. Pittman, and M. R. Boyd, "Tetrazolium-based assays for cellular viability: a critical examination of selected parameters affecting formazan production," Cancer Research, vol. 51, no. 10, pp. 2515-2520, 1991.
  9. J. H. Hoeijmakers, "Genome maintenance mechanisms for preventing cancer," Nature, vol. 411, no. 6835, pp. 366-374, 2001. https://doi.org/10.1038/35077232
  10. D. C. van Gent, J. H. Hoeijmakers, and R. Kanaar, "Chromosomal stability and the DNA double-stranded break connection," Nature Reviews Genetics, vol. 2, no. 3, pp. 196-206, 2001. https://doi.org/10.1038/35056049
  11. M. O'Driscoll, A. R. Gennery, J. Seidel, P. Concannon, and P. A. Jeggo, "An overview of three new disorders associated with genetic instability: LIG4 syndrome, RS-SCID and ATR-Seckel syndrome," DNA Repair, vol. 3, no. 8, pp. 1227-1235, 2004. https://doi.org/10.1016/j.dnarep.2004.03.025
  12. E. P. Rogakou, D. R. Pilch, A. H. Orr, V. S. Ivanova, and W. M. Bonner, "DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139," Journal of Biological Chemistry, vol. 273, no. 10, pp. 5858-5868, 1998. https://doi.org/10.1074/jbc.273.10.5858
  13. T. T. Paull, E. P. Rogakou, V. Yamazaki, C. U. Kirchgessner, M. Gellert, and W. M. Bonner, "A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage," Current Biology, vol. 10, no. 15, pp. 886-895, 2000. https://doi.org/10.1016/S0960-9822(00)00610-2
  14. I. Rappold, K. Iwabuchi, T. Date, and J. Chen, "Tumor suppressor p53 binding protein 1 (53BP1) is involved in DNA damage-signaling pathways," Journal of Cell Biology, vol. 153, no. 3, pp. 613-620, 2001. https://doi.org/10.1083/jcb.153.3.613
  15. M. Lata, J. Prasad, S. Singh, R. Kumar, L. Singh, P. Chaudhary, R. Arora, R. Chawla, S. Tyagi, N. L. Soni, R. K. Sagar, M. Devi, R. K. Sharma, S. C. Puri, and R. P. Tripathi, "Whole body protection against lethal ionizing radiation in mice by REC-2001: a semi-purified fraction of Podophyllum hexandrum," Phytomedicine, vol. 16, no. 1, pp. 47-55, 2009. https://doi.org/10.1016/j.phymed.2007.04.010

Cited by

  1. DNA Spintronics: Charge and Spin Dynamics in DNA Wires vol.120, pp.5, 2016, https://doi.org/10.1021/acs.jpcc.5b09907