Effects of 60-Hz Time-Varying Electric Fields on DNA Damage and Cell Viability Support Negligible Genotoxicity of the Electric Fields

  • Yoon, Yeo Jun (Department of Biochemistry, College of Life Science & Biotechnology, Yonsei University) ;
  • Li, Gen (Department of Biochemistry, College of Life Science & Biotechnology, Yonsei University) ;
  • Kim, Gyoo Cheon (Department of Oral Anatomy, School of Dentistry, Pusan National University) ;
  • Lee, Hae June (Department of Electrical Engineering, Pusan National University) ;
  • Song, Kiwon (Department of Biochemistry, College of Life Science & Biotechnology, Yonsei University)
  • Received : 2015.05.04
  • Accepted : 2015.07.01
  • Published : 2015.07.31


The effect of a 60 Hz time-varying electric field was studied using a facing-electrode device (FED) and a coplanar-electrode device (CED) for further investigation of the genotoxicity of 60 Hz time-varying magnetic field (MF) from preceding research. Neither a single 30-minute exposure to the CED or to the FED had any obvious biological effects such as DNA double strand break (DSB) and apoptosis in cancerous SCC25, and HeLa cells, normal primary fibroblast IMR90 cells, while exposures of 60 Hz time-varying MF led to DNA damage with induced electric fields much smaller than those used in this experiment. Nor did repetitive exposures of three days or a continuous exposure of up to 144 hours with the CED induce any DNA damage or apoptosis in either HeLa or IMR90 cells. These results imply that the solitary electric field produced by time-varying MF is not a major cause of DSBs or apoptosis in cancer or normal cells.


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