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Molecular Screening of Blast Resistance Genes in Rice using SSR Markers

  • Singh, A.K. (Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University) ;
  • Singh, P.K. (Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University) ;
  • Arya, Madhuri (Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University) ;
  • Singh, N.K. (National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute) ;
  • Singh, U.S. (STRASA-IRRI-India Office, Rajendra Place, 9th Floor, Aggrawal Corporate Tower)
  • Received : 2014.06.12
  • Accepted : 2014.11.17
  • Published : 2015.03.01

Abstract

Rice Blast is the most devastating disease causing major yield losses in every year worldwide. It had been proved that using resistant rice varieties would be the most effective way to control this disease. Molecular screening and genetic diversities of major rice blast resistance genes were determined in 192 rice germplasm accessions using simple sequence repeat (SSR) markers. The genetic frequencies of the 10 major rice blast resistance genes varied from 19.79% to 54.69%. Seven accessions IC337593, IC346002, IC346004, IC346813, IC356117, IC356422 and IC383441 had maximum eight blast resistance gene, while FR13B, Hourakani, Kala Rata 1-24, Lemont, Brown Gora, IR87756-20-2-2-3, IC282418, IC356419, PKSLGR-1 and PKSLGR-39 had seven blast resistance genes. Twenty accessions possessed six genes, 36 accessions had five genes, 41 accessions had four genes, 38 accessions had three genes, 26 accessions had two genes, 13 accessions had single R gene and only one accession IC438644 does not possess any one blast resistant gene. Out of 192 accessions only 17 accessions harboured 7 to 8 blast resistance genes.

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