- Volume 16 Issue 1
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Significance of Suppressor of Cytokine Signaling-3 Expression in Bladder Urothelial Carcinoma in Relation to Proinflammatory Cytokines and Tumor Histopathological Grading
- Gaballah, Hanaa Hibishy (Medical Biochemistry Department, Faculty of Medicine, Tanta University) ;
- Shafik, Noha Mohamed (Medical Biochemistry Department, Faculty of Medicine, Tanta University) ;
- Wasfy, Rania Elsayed (Pathology Department, Faculty of Medicine, Tanta University) ;
- Farha, Mohamed Osama Abou (Urology Department, Faculty of Medicine, Tanta University)
- Published : 2015.02.04
Background: Bladder cancer is among the five most common malignancies worldwide. Altered expression of suppressor of cytokine signaling -3 (SOCS-3) has been implicated in various types of human cancers; however, its role in bladder cancer is not well established. Aim: The present study was undertaken to investigate the mRNA expression of SOCS-3 in normal and cancerous bladder tissue and to explore its correlation with urinary levels of some proinflammatory cytokines, cytokeratin-18 (CK -18) and with tumor histopathological grading, in order to evaluate their role as potential diagnostic markers. Materials and Methods: SOCS3 mRNA expression levels were evaluated using quantitative real time PCR. Urinary levels of interleukins 6 and 8 were estimated by enzyme linked immunosorbent assay (ELISA). Cytokeratin-18 expression was analyzed by immuunohistochemistry then validated by ELISA. Results: SOC3 m RNA expression levels were significantly lower in high grade urothelial carcinoma (
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