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Role of +405C>G and +936C>T Polymorphisms of the Vascular Endothelial Growth Factor Gene and Risk of Esophageal Cancer in the Kashmiri Population

  • Qasim, Iqbal (Department of Immunology and Molecular Medicine Sheri Kashmir Institute of Medical Sciences) ;
  • Bhat, Imtiyaz Ahmad (Department of Immunology and Molecular Medicine Sheri Kashmir Institute of Medical Sciences) ;
  • Masoodi, Khalid Zafar (Department of Immunology and Molecular Medicine Sheri Kashmir Institute of Medical Sciences) ;
  • Shah, Zafar Amin (Department of Immunology and Molecular Medicine Sheri Kashmir Institute of Medical Sciences)
  • Published : 2015.02.04

Abstract

Background: The gene for the vascular endothelial growth factor (VEGF), which promotes angiogenesis and permeability, is polymorphic. The aim of the present study was to evaluate the relationship between +936C>T and +404C>G polymorphism of VEGF with risk of esophageal cancer in the Kashmiri population in India. Materials and Methods: 150 esophageal cancer patients and 150 unrelated healthy controls were genotyped for two VGEF SNPs (+405C/G, and +936C/T) using DNA extracted from prospectively collected blood samples by the PCR-RFLP method. Results: For the VEGF +936C>T polymorphism a significant association of CT and combined CT+TT genotypes was observed with increased risk of esophageal cancer (p=0.021; 0.024). For the +405C>G polymorphism we observed significantly increased frequency of GG genotype in cases as compared to controls and also the +405 GG Genotype was observed to have a two fold risk(OR=2.7356; 95%CI=1.1409-6.5593; p=0.020). The combined genotypes of GG-CC and GG-CT of +405C>G and +936C>T were found to be significantly associated with increased risk of esophageal cancer (p=0.0376; 0.0099). Conclusions: From the results of the present study a significant association of +936C>T and +405C>G polymorphisms with increased esophageal cancer risk exists in the Kashmiri population.

Keywords

Esophageal cancer;VEGF;polymorphisms;angiogenesis;Kashmir;India

Acknowledgement

Supported by : ICMR

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