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Evaluation of BCL6 and MUM1 Expression in Patients with Diffuse Large B cell Lymphoma and their Correlations with Staging and Prognosis in Iran

  • Rahimi, Hossein (Hematology Oncology Department, Mashhad University of Medical Science) ;
  • Jafarian, Amirhossein (Pathology Department, Cancer and Molecular Pathology Research Center, Mashhad University of Medical Science) ;
  • Samadi, Alireza (Internal Medicine Research Center for Patient Safety, Mashhad University of Medical Science) ;
  • Meamar, Bahram (Pathology, Cancer Research Center, Mashhad University of Medical Science) ;
  • Rahmani, Shaghayegh (Research Center for Patient Safety, Mashhad University of Medical Science)
  • Published : 2015.02.04

Abstract

Background: Diffuse large B-cell lymphoma (DLBCL) is the most common form of non-Hodgkins lymphoma (NHL), accounting for approximately 25% of NHL cases. The aim of this study was to evaluate the association between the BCL6 and MUM1 gene expression and patient prognosis and stage. Materials and Methods: After ethical approval, in a cross-sectional study, tissue samples of 80 patients with diffuse large B-cell lymphoma were analyzed for BCL6 and MUM1 gene expression. Immunohistochemical staining was performed with division into categories of 0-5%, 5-25%, 26-50%, 51-75% and more than 75%. Other clinical and histological information such as lymph node involvement, T-stage, B symptoms and patient outcome were also recorded. Data were analyzed with SPSS version 16 and a P-value less than 0.05 was considered significant. Results: The patient mean age was $46.9{\pm}10.5$ years ($47.6{\pm}10.7$ and $46.1{\pm}9.6$ for males and females, respectively). A significant association was seen between lymphoma stage and BCL6 (p=0.045) but not MUM1 expression (p=0.09). However, the latter was associated with mortality (p=0.006) as was also the BCL6 level (p=0.006). Conclusions: : Overexpression of MUM1 and BCL6 is associated with poor prognosis in patients with diffuse large B-cell lymphoma.

Keywords

Diffuse large B-cell lymphoma;MUM1;BCL6;prognosis;Iran

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