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Human Telomerase Gene and High-Risk Human Papillomavirus Infection are Related to Cervical Intraepithelial Neoplasia

  • Zhao, Xu-Ye (Department of Gynecology and Obstetrics, Chinese PLA General Hospital & Chinese PLA Medical School) ;
  • Cui, Yongm (Department of Pathophysiology, Binzhou Medical University) ;
  • Jiang, Shu-Fang (Department of Gynecology and Obstetrics, Chinese PLA General Hospital & Chinese PLA Medical School) ;
  • Liu, Ke-Jun (Department of Gynecology and Obstetrics, Chinese PLA General Hospital & Chinese PLA Medical School) ;
  • Han, Hai-Qiong (Department of Gynecology, Shanxi Tumor Hospital) ;
  • Liu, Xiao-Su (Department of Gynecology, Shanxi Tumor Hospital) ;
  • Li, Yali (Department of Gynecology and Obstetrics, Chinese PLA General Hospital & Chinese PLA Medical School)
  • Published : 2015.02.25

Abstract

Our aims were to evaluate the clinical performance of human telomerase RNA gene component (hTERC gene) amplification assay with high-risk human papillomavirus (HR-HPV) DNA test of Hybrid Capture 2 DNA test (HC2), for the detection of high grade cervical precancerous lesions and cancer (CIN 2+). In addition, the association shown between hTERC gene amplification and HPV DNA test positive in women with and without cervical neoplasia was assessed. There were 92 women who underwent cytology, HR-HPV DNA test, hTERC gene amplification test, colposcopy and biopsy. We compared the clinical performance of hTERC gene test along with HR-HPV DNA test of women with colposcopy and routine screening. The samples were histology-confirmed high-grade cervical intraepithelial neoplasia (CIN 2) or worse (CIN2+) as the positive criterion. The test of hTERC gene showed the hTERC gene amplification positivity increased with the severity of histological abnormality and cytological abnormality. The test of hTERC gene showed higher specificity than HR-HPV DNA test for high-grade lesions (84.4% versus 50%) and also higher positive predictive value (90.4% versus 76.5%). Our results predicted that hTERC gene amplification demonstrated more specific performance for predicting the risk of progression and offer a strong potential as a tool for triage in cervical cancer screening, with the limited sensitive as HR-HPV DNA test.

Acknowledgement

Supported by : Chinese People's Liberation Army General Hospital

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