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Polymorphisms in Epigenetic and Meat Quality Related Genes in Fourteen Cattle Breeds and Association with Beef Quality and Carcass Traits

  • Liu, Xuan (Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University) ;
  • Usman, Tahir (Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University) ;
  • Wang, Yachun (Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University) ;
  • Wang, Zezhao (Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University) ;
  • Xu, Xianzhou (Dalian Xuelong Industry Limited Group) ;
  • Wu, Meng (Dalian Xuelong Industry Limited Group) ;
  • Zhang, Yi (Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University) ;
  • Zhang, Xu (Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University) ;
  • Li, Qiang (Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University) ;
  • Liu, Lin (Beijing Dairy Cattle Center) ;
  • Shi, Wanhai (Beijing Dairy Cattle Center) ;
  • Qin, Chunhua (Ningxia Sygen BioEngineering Research Center) ;
  • Geng, Fanjun (Dingyuan Seedstock Bulls Breeding Ltd.) ;
  • Wang, Congyong (Dingyuan Seedstock Bulls Breeding Ltd.) ;
  • Tan, Rui (Xinjiang General Livestock Service) ;
  • Huang, Xixia (College of Animal Science, Xinjiang Agriculture University) ;
  • Liu, Airong (Xiertala Breeding Farm, Hailaer Farm Buro) ;
  • Wu, Hongjun (Hailaer Farm Buro) ;
  • Tan, Shixin (Xinjiang Tianshan Animal Husbandry Bio-Eng. Co. Ltd.) ;
  • Yu, Ying (Key Laboratory of Agricultural Animal Genetics and Breeding, National Engineering Laboratory for Animal Breeding, College of Animal Science and Technology, China Agricultural University)
  • Received : 2013.12.21
  • Accepted : 2014.08.14
  • Published : 2015.04.01

Abstract

Improvement for carcass traits related to beef quality is the key concern in beef production. Recent reports found that epigenetics mediates the interaction of individuals with environment and nutrition. The present study was designed to analyze the genetic effect of single nucleotide polymorphisms (SNPs) in seven epigenetic-related genes (DNMT1, DNMT3a, DNMT3b, DNMT3L, Ago1, Ago2, and HDAC5) and two meat quality candidate genes (CAPN1 and PRKAG3) on fourteen carcass traits related to beef quality in a Snow Dragon beef population, and also to identify SNPs in a total of fourteen cattle populations. Sixteen SNPs were identified and genotyped in 383 individuals sampled from the 14 cattle breeds, which included 147 samples from the Snow Dragon beef population. Data analysis showed significant association of 8 SNPs within 4 genes related to carcass and/or meat quality traits in the beef populations. SNP1 (13154420A>G) in exon 17 of DNMT1 was significantly associated with rib-eye width and lean meat color score (p<0.05). A novel SNP (SNP4, 76198537A>G) of DNMT3a was significantly associated with six beef quality traits. Those individuals with the wild-type genotype AA of DNMT3a showed an increase in carcass weight, chilled carcass weight, flank thicknesses, chuck short rib thickness, chuck short rib score and in chuck flap weight in contrast to the GG genotype. Five out of six SNPs in DNMT3b gene were significantly associated with three beef quality traits. SNP15 (45219258C>T) in CAPN1 was significantly associated with chuck short rib thickness and lean meat color score (p<0.05). The significant effect of SNP15 on lean meat color score individually and in combination with each of other 14 SNPs qualify this SNP to be used as potential marker for improving the trait. In addition, the frequencies of most wild-type alleles were higher than those of the mutant alleles in the native and foreign cattle breeds. Seven SNPs were identified in the epigenetic-related genes. The SNP15 in CAPN1 could be used as a powerful genetic marker in selection programs for beef quality improvement in the Snow Dragon Beef population.

Keywords

Association Study;Epigenetic-related Genes;CAPN1;Protein Kinase Subunit Gamma-3 Gene PRKAG3;Beef Quality;Carcass Traits

Acknowledgement

Supported by : National Natural Science Foundation of China

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