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Motility, Fertilizability and Subsequent Embryonic Development of Frozen-thawed Spermatozoa derived from Epididymis in Hanwoo

  • Yang, Byoung-Chul (Hanwoo Research Institute, National Institute of Animal Science, RDA) ;
  • Kang, Sung-Sik (Hanwoo Research Institute, National Institute of Animal Science, RDA) ;
  • Park, Chang-Seok (Hanwoo Research Institute, National Institute of Animal Science, RDA) ;
  • Kim, Ui-Hyung (Hanwoo Research Institute, National Institute of Animal Science, RDA) ;
  • Kim, Hyeong-Cheol (Hanwoo Research Institute, National Institute of Animal Science, RDA) ;
  • Jeon, Gi-Jun (Hanwoo Research Institute, National Institute of Animal Science, RDA) ;
  • Kim, Sidong (Hanwoo Research Institute, National Institute of Animal Science, RDA) ;
  • Lee, Seok-Dong (Hanwoo Research Institute, National Institute of Animal Science, RDA) ;
  • Lee, Hyun-Jae (Hanwoo Research Institute, National Institute of Animal Science, RDA) ;
  • Cho, Sang-Rae (Hanwoo Research Institute, National Institute of Animal Science, RDA)
  • Received : 2015.11.19
  • Accepted : 2015.12.21
  • Published : 2015.12.31

Abstract

The aim of the study was to investigate the ability of sperm derived from the epididymis in regard to sperm motility, sperm penetration to oocyte and subsequent development of the embryo. Frozen-thawed sperm from epididymis showed similar percentage of motile sperm (VSL ${\geq}25{\mu}m/sec$) as compared to that of commercial sperm (control). Sperm penetration of frozen-thawed epididymal and commercial sperm was not significantly different. Moreover, cleavage and blastocyst rates were similar in both epididymal and control. Sperm derived from the epididymis also showed fertilizability and subsequent embryonic development.

Keywords

Acknowledgement

Grant : Development of the methods to control the genetic diversity and to improve the selection effect in Hanwoo line population

Supported by : Rural Development Administration

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