Association with Post-Thawed Semen Motility and Kinematic Characteristics of g.35756 T>C on Estrogen Receptor 1 (ESR1) Gene in Duroc Pigs

두록의 동결정액의 운동학적 특성과 ESR1 유전자의 SNP(g.35756T>C)와 연관성 분석

  • Cho, Eun-Seok (National Institute of Animal Science, Rural Development Adminstration) ;
  • Kim, Ki-Hyun (National Institute of Animal Science, Rural Development Adminstration) ;
  • Woo, Jae-Seok (National Institute of Animal Science, Rural Development Adminstration) ;
  • Lee, Mi-Jin (National Institute of Animal Science, Rural Development Adminstration) ;
  • Ko, Jun-Ho (National Institute of Animal Science, Rural Development Adminstration) ;
  • Kim, Young-Ju (National Institute of Animal Science, Rural Development Adminstration) ;
  • Sa, Soo-Jin (National Institute of Animal Science, Rural Development Adminstration)
  • 조은석 (농촌진흥청 국립축산과학원) ;
  • 김기현 (농촌진흥청 국립축산과학원) ;
  • 우제석 (농촌진흥청 국립축산과학원) ;
  • 이미진 (농촌진흥청 국립축산과학원) ;
  • 고준호 (농촌진흥청 국립축산과학원) ;
  • 김영주 (농촌진흥청 국립축산과학원) ;
  • 사수진 (농촌진흥청 국립축산과학원)
  • Received : 2015.09.22
  • Accepted : 2015.09.24
  • Published : 2015.09.30


Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Various researches have been trying to improve the quality of semen Post-thawed in boar. Recently, polymorphism (g. 35756 T>C) of Estrogen Receptor 1 (ESR1) gene reported to be significant association with MOT. This study was conducted to evaluate the ESR1 gene as a positional controlling for motility and kinematic characteristics of post-thawed boar semen. To results, The g.35756 T>C SNP of ESR1 was significantly associated with frozen semen motility and kinematic characteristics. The g.35756 T>C SNP was high significantly associated with MOT, VCL, VSL and VAP (p<0.001). The SNP was also significantly associated with ALH (P<0.05). Therefore, we suggest that the g. 35756 T>C polymorphism in the intron 1 region of the porcine ESR1 gene could potentially be applied in frozen semen programs to improve MOT trait, but only after validation in other populations.


Supported by : Rural Development Administration


  1. Capon F, Allen MH, Ameen M, Burden AD, Tillman D, Barker JN and Trembath RC. 2004. A synonymous SNP of the corneodesmosin gene leads to increased mRNA stability and demonstrates association with psoriasis across diverse ethnic groups. Human Molecular Genetics. 13(20):2361-2368.
  2. Carreau S, Bourguiba S, Lambard S, Galeraud-Denis I, Genissel C and Levallet J. 2002. Reproductive system: Aromatase and estrogens. Molecular and Cellular Endocrinology. 193(1):137-143.
  3. Chen X, Zhu H, Hu C, Hao H, Zhang J, Li K, Zhao X, Qin T, Zhao K and Zhu H. 2014. Identification of differentially expressed proteins in fresh and frozen-thawed boar spermatozoa by iTRAQ-coupled 2D LC-MS/MS. Reproduction. 147(3):321-330.
  4. Couse JF and Korach KS. 1999. Estrogen receptor null mice: what have we learned and where will they lead us? Endocrine Reviews. 20(3):358-417.
  5. Diniz D, Lopes M, Broekhuijse M, Lopes P, Harlizius B, Guimaraes S, Duijvesteijn N, Knol E and Silva F. 2014. A genomewide association study reveals a novel candidate gene for sperm motility in pigs. Animal Reproduction Science. 151(3):201-207.
  6. Eddy E, Washburn T, Bunch D, Goulding E, Gladen B, Lubahn D and Korach K. 1996. Targeted disruption of the estrogen receptor gene in male mice causes alteration of spermatogenesis and infertility. Endocrinology. 137(11):4796-4805.
  7. Gunawan A, Cinar M, Uddin M, Kaewmala K, Tesfaye D, Phatsara C, Tholen E, Looft C and Schellander K. 2012. Investigation on association and expression of ESR2 as a candidate gene for boar sperm quality and fertility. Reproduction in Domestic Animals. 47(5):782-790.
  8. Hess RA, Gist DH, Bunick D, Lubahn DB, Farrell A, Bahr J, Cooke PS and Greene GL. 1997. Estrogen receptor($\alpha$ and $\beta$) expression in the excurrent ducts of the adult male rat reproductive tract. Journal of Andrology. 18(6):602-611.
  9. Johnson L. 1998. Current developments in swine semen: Preservation, artificial insemination and sperm sexing.
  10. Kaewmala K, Uddin M, Cinar M, Grosse Brinkhaus C, Jonas E, Tesfaye D, Phatsara C, Tholen E, Looft C and Schellander K. 2012. Investigation into association and expression of PLCz and COX 2 as candidate genes for boar sperm quality and fertility. Reproduction in Domestic Animals. 47(2):213-223.
  11. Kuiper G, Enmark E, Pelto-Huikko M, Nilsson S and Gustafsson J-A. 1996. Cloning of a novel receptor expressed in rat prostate and ovary. Proceedings of the National Academy of Sciences. 93(12):5925-5930.
  12. Lazaros LA, Xita NV, Kaponis AI, Zikopoulos KA, Plachouras NI and Georgiou IA. 2010. Estrogen receptor $\alpha$ and $\beta$ polymorphisms are associated with semen quality. Journal of Andrology. 31(3):291-298.
  13. Maes D, Nauwynck H, Rijsselaere T, Mateusen B, Vyt P, de Kruif A and Van Soom A. 2008. Diseases in swine transmitted by artificial insemination: An overview. Theriogenology. 70(8):1337-1345.
  14. Mazur P. 1984. Freezing of living cells: Mechanisms and implications. American Journal of Physiology-Cell Physiology. 247(3):C125-C142.
  15. Pagani F and Baralle FE. 2004. Genomic variants in exons and introns: Identifying the splicing spoilers. Nature Reviews Genetics. 5(5):389-396.
  16. Safarinejad MR, Shafiei N and Safarinejad S. 2010. Association of polymorphisms in the estrogen receptors alpha, and beta (ESR1, ESR2) with the occurrence of male infertility and semen parameters. The Journal of Steroid Biochemistry and Molecular Biology. 122(4):193-203.
  17. Solakidi S, Psarra AG, Nikolaropoulos S and Sekeris CE. 2005. Estrogen receptors $\alpha$ and $\beta$($ER\alpha$ and $ER\beta$) and androgen receptor(AR) in human sperm: Localization of $ER\beta$ and AR in mitochondria of the midpiece. Human Reproduction. 20(12):3481-3487.
  18. Suzuki Y, Sasagawa I, Itoh K, Ashida J, Muroya K and Ogata T. 2002. Estrogen receptor alpha gene polymorphism is associated with idiopathic azoospermia. Fertility and Sterility. 78(6):1341-1343.
  19. Xing Y, Ren J, Ren D, Guo Y, Wu Y, Yang G, Mao H, Brenig B and Huang L. 2009. A whole genome scanning for quantitative trait loci on traits related to sperm quality and ejaculation in pigs. Animal Reproduction Science. 114(1):210-218.
  20. Zeng C, He L, Peng W, Ding L, Tang K, Fang D and Zhang Y. 2014. Selection of optimal reference genes for quantitative RT-PCR studies of boar spermatozoa cryopreservation. Cryobiology. 68(1):113-121.