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Association Study Analysis of Phospholipase C Zeta (PLCz) Gene Polymorphism (g.158T>C) for Duroc Boar Post-Thawed Semen Motility and Kinematic Characteristics

PLCz 유전자의 유전적 다형성(g.158T>C)과 두록 동결정액의 운동학적 특성과의 연관성 분석

  • Sa, Soo-Jin (National Institute of Animal Science, Rural Development Adminstration) ;
  • Lee, Mi-Jin (National Institute of Animal Science, Rural Development Adminstration) ;
  • Kim, Ki-Hyun (National Institute of Animal Science, Rural Development Adminstration) ;
  • Woo, Jae-Seok (National Institute of Animal Science, Rural Development Adminstration) ;
  • Ko, Jun-Ho (National Institute of Animal Science, Rural Development Adminstration) ;
  • Kim, Young-Ju (National Institute of Animal Science, Rural Development Adminstration) ;
  • Cho, Eun-Seok (National Institute of Animal Science, Rural Development Adminstration)
  • 사수진 (농촌진흥청 국립축산과학원) ;
  • 이미진 (농촌진흥청 국립축산과학원) ;
  • 김기현 (농촌진흥청 국립축산과학원) ;
  • 우제석 (농촌진흥청 국립축산과학원) ;
  • 고준호 (농촌진흥청 국립축산과학원) ;
  • 김영주 (농촌진흥청 국립축산과학원) ;
  • 조은석 (농촌진흥청 국립축산과학원)
  • Received : 2015.09.10
  • Accepted : 2015.09.22
  • Published : 2015.09.30

Abstract

Cryopreservation of boar semen is continually researched in reproductive technologies and genetic resource banking in breed conservation. For evaluating the boar semen quality, sperm motility (MOT) is an important parameter because the movement of spermatozoa indicates active metabolism, membrane integrity and fertilizing capacity. Various researches have been trying to improve the quality of semen post-thawed in boar. Recently, polymorphism (g.158T>C) of phospholipase C zeta (PLCz) gene reported to be significant association with MOT. This study was conducted to evaluate the PLCz gene as a positional controlling for motility and kinematic characteristics of post-thawed boar semen. To results, The g.158 T>C SNP of PLCz was significantly associated with frozen semen motility and kinematic characteristics. g.158 T>C SNP was high significantly associated with MOT, VCL, VSL and VAP (p<0.0001, p=0.0002, p<0.0001 and p<0.0001, respectively). Therefore, we suggest that the intron region of the porcine PLCz, may be used as a molecular marker for Duroc boar post-thawed semen quality, although its functional effect was not defined yet. Whether the association is due to the candidate gene or not require further verification. Thus, it will be of interest to continue association studies in the regions surrounding those genes.

Acknowledgement

Grant : PRRS 저항성 마커를 이용한 돼지 생산성 향상 기술 개발

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