DOI QR코드

DOI QR Code

Anti-inflammatory Effect of Water Extract from Tuna Heart on Lipopolysaccharide-induced Inflammatory Responses in RAW 264.7 Cells

Lipopolysaccharide로 유도된 RAW 264.7 세포에 대한 참치심장 물 추출물의 항염증 효과

  • Kim, Min-Ji (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Bae, Nan-Young (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Koth-Bong-Woo-Ri (Institute of Fisheries Sciences, Pukyong National University) ;
  • Park, Ji-Hye (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Park, Sun-Hee (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Cho, Young-Je (School of Food Science of Biotechnology, Kyungpook National University) ;
  • Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
  • 김민지 (부경대학교 식품공학과/식품연구소) ;
  • 배난영 (부경대학교 식품공학과/식품연구소) ;
  • 김꽃봉우리 (부경대학교 수산과학연구소) ;
  • 박지혜 (부경대학교 식품공학과/식품연구소) ;
  • 박선희 (부경대학교 식품공학과/식품연구소) ;
  • 조영제 (경북대학교 식품공학부) ;
  • 안동현 (부경대학교 식품공학과/식품연구소)
  • Received : 2015.10.16
  • Accepted : 2015.12.17
  • Published : 2015.12.27

Abstract

The anti-inflammatory effect of tuna heart water extract (THWE) was investigated using lipopolysaccharide-induced inflammatory response in this study. Anti-inflammatory effect was detected by the cell proliferation and the production levels of nitric oxide, pro-inflammatory cytokines such as interleukin-6 (IL-6), IL-$1{\beta}$, and tumor necrosis factor-alpha. As a result, there were no cytotoxic effects on proliferation of macrophages treated with THWE compared to the control. The production of pro-inflammatory cytokines was remarkably suppressed compared with that of the LPS only group. These results suggest that THWE exerts the anti-inflammatory property by inhibiting production of inflammatory factors and may be a potential material for anti-inflammatory therapy.

Acknowledgement

Grant : 수산가공부산물을 이용한 고부가가치 소재 및 식품 개발

Supported by : 한국해양과학기술진흥원

References

  1. Rocca, B. and G. A. FitzGerald (2002) Cyclooxygenases and prostaglandins: Shaping up the immune response. Int. Immunopharacol. 2: 603-630. https://doi.org/10.1016/S1567-5769(01)00204-1
  2. Lawrence, T., D. A. Willoughby, and D. W. Gilroy (2002) Antiinflammatory lipid mediators and insights into the resolution of inflammation. Nat. Rev. Immunol. 2: 787-795. https://doi.org/10.1038/nri915
  3. Jeong, J. B., S. C. Hong, H. J. Jeong, and J. S. Koo (2012) Antiinflammatory effects of ethyl acetate fraction from Cnidium officinale Makino on LPS-stimulated RAW 264.7 and THP-1 cells. Korean J. Plant Res. 25: 299-307. https://doi.org/10.7732/kjpr.2012.25.3.299
  4. Jeong, D. H., K. B. W. R. Kim, M. J. Kim, B. K. Kang, and D. H. Ahn (2014) Anti-inflammatory activity of methanol extract and nhexane fraction mojabanchromanol b from Myagropsis myagroides. Life Sci. 114: 12-19. https://doi.org/10.1016/j.lfs.2014.07.036
  5. Rankin, J. A. (2004) Biological mediators of acute inflammation. AACN Clin. Issues 15: 3-17. https://doi.org/10.1097/00044067-200401000-00002
  6. Guzik, T. J., R. Korbut, and T. Adamek-Guzik (2003) Nitric oxide and superoxide in inflammation and immune regulation. J. Physiol. Pharmacol. 54: 469-487.
  7. Masaki, M., M. Matsushita, and K. Wakitani (1998) Inhibitory effect of JTE-522, a novel prostaglandin H synthase-2 inhibitor, on adjuvant-induced arthritis and bone changes in rats. Inflamm. Res. 47: 187-192. https://doi.org/10.1007/s000110050316
  8. Pedersen, M. H., C. Molgaard, L. I. Hellgren, and L. Lauritzen (2010) Effects of fish oil supplementation on markers of the metabolic syndrome. J. Pediatr. 157: 395-400. https://doi.org/10.1016/j.jpeds.2010.04.001
  9. Tenore, G. G., G. Calabrese, A. Ritieni, P. Campiglia, D. Gianetti, and E. Novellino (2014) functional food potentially safer than commercial fish oil based pharmaceutical formulations. Food Chem. Toxicol. 71: 231-235. https://doi.org/10.1016/j.fct.2014.06.016
  10. McGahon, B. M., D. S. D. Martin, D. F. Horrobin, and M. A. Lynch (1999) Age-related changes in synaptic function: analysis of the effect of dietary supplementation with $\omega$-3 fatty acids. Neurosci. 94: 315-314. https://doi.org/10.1016/S0306-4522(99)00242-0
  11. Jang, J. R., K. K. Kim, S. B. Mun, and S. Y. Lim (2009) In vitro anticancer and antioxidant effect of solvent extracts from tuna dried at low temperature vacuum. J. Life Sci. 19: 633-638. https://doi.org/10.5352/JLS.2009.19.5.633
  12. Hunter, E. (1987) PUFA and eicosanoid research. J. Anim. Oil Chem. Soc. 64: 1088-1092. https://doi.org/10.1007/BF02612980
  13. Hwang, W. I., N. G. Baik, Y. K. Hwang, and S. D. Lee (1992) Antitumor and immunological effects of tuna extract. J. Korean Soc. Food Nutr. 21: 353-366.
  14. Nestel, P. J. (1987) Polyunsaturated fatty acids (n-3, n-6). Am. J. Clin. Nutr. 45: 1161-1167 https://doi.org/10.1093/ajcn/45.5.1161
  15. Gnez-Guillnm, M. C., M. E. Lpez-Caballero, A. Alemn, A. Lpez de Lacey, B. Gimnez, and P. Montero (2010) Antioxidant and antimicrobial peptide fractions from squid and tuna skin gelatin. pp. 89-115. In: Le Bihan E. ed. Sea by-products as real material: New ways of application. Transworld Research Network, Trivandrum, India.
  16. Kim, Y. M., I. H. Kim, J. W. Choi, M. K. Lee, and T. J. Nam (2015) The anti-obesity effects of a tuna peptide on 3T3-L1 adipocytes are mediated by the inhibition of the expression of lipogenic and adipogenic genes and by the activation of the Wnt/${\beta}$-catenin signaling pathway. Int. J. Mol. Med. 36:327-334. https://doi.org/10.3892/ijmm.2015.2231
  17. Ministry of Oceans and Fisheries (2003) Fisheries statistics yearbook. pp. 150-172. Korea.
  18. Kang, C. H., H. Y. Jung, D. H. Lee, J. K, Park, J. H. Ha, S. C. Lee, and Y. I. Hwang (2000) Analysis of chemical compounds on tuna processing by-products. J. Korean Soc. Food Sci. Nutr. 29: 981-986.
  19. Park, S. E., H. W. Kim, S. R. Lee, and B. K. Kim (2000) Effects of nucleic acids complex of tuna testis on immunological activities. J. Korean Assoc. Cancer Prev. 5: 15-23.
  20. Shin, M. O., M. J. Ku, and S. J. Bae (2007) Cytotoxicity and quinone reductase activity stimulating effects of fin of Thunnus thynnus extracts in various cancer cells. Korean J. Nutr. 40: 147-153.
  21. Lee, H. S., H. J. Kim, J. I. Choi, J. H. Kim, J. G. Kim, B. S. Chun, D. H. Ahn, Y. J. Chung, Y. J. Kim, M. W. Byun, and J. W. Lee (2008) Antioxidant activity of the ethanol extract from cooking drips of Thunnus thynnus by gamma irradiation. J. Korean Soc. Food Sci. Nutr. 37: 810-814. https://doi.org/10.3746/jkfn.2008.37.6.810
  22. Cheng, M. L., H. C. Wang, K. C. Hsu, and J. S. Hwang (2015) Anti-inflammatory peptides from enzymatic hydrolysates of tuna cooking juice. Food Agric. Immunol. 26: 770-781. https://doi.org/10.1080/09540105.2015.1036352
  23. Park, Y. M., J. H. Won, K. J. Yun, J. H. Ryu, Y. N. Han, S. K. Choi, and K. T. Lee (2006) Preventive effect of Ginkgo biloba extract (GBB) on the lipopolysaccharide-induced expressions of inducible nitric oxide synthase and cyclooxygenase-2 via suppression of nuclear factor-${\kappa}B$ in RAW 264.7 cells. Biol. Pharm. Bull. 29: 985-990. https://doi.org/10.1248/bpb.29.985
  24. Lee, S. T., Y. R. Jeong, M. H. Ha, S. H. Kim, M. W. Byun, and S. K. Jo (2000) Induction of nitric oxide and TNF-${\alpha}$by herbal plant extracts in mouse macrophages. J. Korean Soc. Food Sci. Nutr. 29: 342-348.
  25. Kang, B. K., M. J. Kim, K. B. W. R. Kim, N. K. Ahn, Y. U. Choi, S. W. Bark, W. M. Pak, B. R. Kim, J. H. Park, N. Y. Bae, and D. H. Ahn (2015) The anti-inflammatory effect of skipjack tuna (Katsuwonus pelamis) oil in LPS-induced RAW 264.7 cells and mouse models. Microbiol. Biotechnol. Lett. 43: 45-55. https://doi.org/10.4014/mbl.1412.12001
  26. Kang, B. K., K. B. W. R. Kim, N. K. Ahn, Y. U. Choi, M. J. Kim, S. W. Bark, W. M. Pak, B. R. Kim, J. H. Park, N. Y. Bae, and D. H. Ahn (2014) Immuno-stimulating activities of skipjack tuna Katsuwounus pelamis cooking juice concentrates on mouse macrophages and spleen cells. Kor. J. Fish. Aquat. Sci. 47: 776-784.
  27. Evans, C. H. (1995) Nitric oxide: what role does it play in inflammation and tissue destruction. Agents Actions Suppl. 47: 107-116.
  28. Kubes, P. and D. M. McCafferty (2000) Nitric oxide and intestinal inflammation. Am. J. Med. 109: 150-158. https://doi.org/10.1016/S0002-9343(00)00480-0
  29. Kim, J. Y., K. S. Jung, and H. G. Jeong (2004) Suppressive effects of the kahweol and cafestol on cyclooxygenase-2 expression in macrophages. FEBS Lett. 569: 321-326. https://doi.org/10.1016/j.febslet.2004.05.070
  30. Seo, J. S., T. H. Lee, S. M. Lee, S. E. Lee, N. S. Seong, and J. Y. Kim (2009) Inhibitory effects of methanolic extracts of medicinal plants on nitric oxide production in activated macrophage RAW 264,7 cells. Korean J. Medicinal Crop Sci. 17: 173-178.
  31. Weisz, A., I. Cicatiello, and H. Esumi (1996) Regulation of the mouse inducible-type nitric oxide synthase gene promoter by interferon- gamma, bacterial lipopolysaccharide and NG-monomethyl- L-arginine. Biochem J. 316: 209-215. https://doi.org/10.1042/bj3160209
  32. Jeong, D. H. (2013) Anti-inflammatory activity of the tuna eyeball oil and Sargassum micracanthum extracts. Master Thesis. Pukyong National University, Busan, Korea.
  33. Khair-El-Din, T., S. C. Sicher, M. A. Vazquez, G. W. Chung, K. A. Stallworth, K. Kitamura, R. T. Miller, and C. Y. Lu (1996) Transcription of the murine iNOS gene is inhibited by docosahexaenoic acid, a major constituent of fetal and neonatal sera as well as fish oils. J. Exp. Med. 183: 1241-1246. https://doi.org/10.1084/jem.183.3.1241
  34. Hwang, J. W., S. J. Lee, Y. S. Kim, E. K. Kim, C. B. Ahn, Y. J. Jeon, S. H. Moon, B. T. Jeon, and P. J. Park (2012) Purification and characterization of a novel peptide with inhibitory effects on colitis induced mice by dextran sulfate sodium from enzymatic hydrolysates of Crussostrea gigas. Fish Shellfish Immunol. 33: 993-999. https://doi.org/10.1016/j.fsi.2012.08.017
  35. Lee, S. J., E. K. Kim, Y. S. Kim, J. W. Hwang, K. H. Lee, D. K. Choi, H. Kang, S. H. Moon, B. T. Jeon, and P. J. Park (2012) Purification and characterization of a nitric oxide inhibitory peptide from Ruditupes philippinarum. Food Chem. Toxicol. 50: 1660-1666. https://doi.org/10.1016/j.fct.2012.02.021
  36. Majdalawieh, A. and H. S. Ro (2010) Regulation of $I{\kappa}B{\alpha}$ function and NF-${\kappa}B$ signaling: AEBP1 is a novel proinflammatory mediator in macrophages. Mediators Inflamm. 2010: 1-27.
  37. Tizard, I. R. (1988) Immunology: An introduction. 2nd ed., pp. 423-441. Saunders College Publishing, New York, NY, USA.
  38. Delgado, A. V., A. T. McManus, and J. P. Chambers (2003) Production of tumor necrosis factor-alpha, interleukin 1-beta, interleukin 2 and interleukin 6 by rat leukocyte subpopulations after exposure to substance P. Neuropeptides. 37: 355-361. https://doi.org/10.1016/j.npep.2003.09.005
  39. Nathan, C. (1992) Nitric oxide as a secretory product of mammalian cells. FASEB J. 6: 3051-3064. https://doi.org/10.1096/fasebj.6.12.1381691
  40. Walsh, L. J., G. Trinchieri, H. A. Waldorf, D. Whitaker, and G. F. Murphy (1991) Human dermal mast cells contain and release tumor necrosis factor alpha, which induces endothelial leukocyte adhesion molecule 1. Proc. Natl. Acad. Sci. USA 88: 4220-4224. https://doi.org/10.1073/pnas.88.10.4220
  41. Dinarello, C. A. (1999) Cytokines as endogenous pyrogens. J. Infect. Dis. 179: 294-304. https://doi.org/10.1086/314577
  42. Lebovic, D. I., F. Bentzien, V. A. Chao, E. N. Garrett, Y. G. Meng and R. N. Taylor (2000) Induction of an angiogenic phenotype in endometriotic stromal cell cultures by interleukin-1beta. Mol. Hum. Reprod. 6: 269-275. https://doi.org/10.1093/molehr/6.3.269
  43. Lin, M. C., S. B. Lin, S. C. Lee, C. C. Lin, C. F. Hui, and J. Y. Chen (2010) Antimicrobial peptide of an antilipopolysaccharide factor modulates of the inflammatory response in RAW264.7 cells. Peptides 31: 1262-1272. https://doi.org/10.1016/j.peptides.2010.04.004
  44. Kang, B. K. (2015) Anti-inflammatory activity of Sargassum fulvellum and skipjack tuna (Katsuwonus pelamis) heart ethanol extract and purification of grasshopper ketone. Master Thesis. Pukyong National University, Busan, Korea.

Cited by

  1. on pro-inflammatory responses in lipopolysaccharide-induced Raw 264.7 Cells vol.60, pp.4, 2017, https://doi.org/10.3839/jabc.2017.057
  2. Inhibitory Activity of Sargassum hemiphyllum Ethanol Extract on Inflammatory Response in LPS-induced RAW 264.7 Cells and Mouse Model vol.32, pp.4, 2017, https://doi.org/10.7841/ksbbj.2017.32.4.319