Development of Effective Screening Method for Efficacy Test of Trunk Injection Agents Against Pine Wood Nematode, Bersaphelenchus xylophilus in Japanese Black Pine, Pinus thunbergii

곰솔(Pinus thunbergii)에서 소나무재선충(Bersaphelenchus xylophilus) 나무주사 약제의 효율적인 평가법 개발

  • Shin, Won Sik (Department of Applied Biology, School of Environmental Ecology and Tourism, Kyungpook National University) ;
  • Jung, Young Hack (Devision of Applied Life Science (BK21 program), Department of Applied Biology, Gyeongsang National University) ;
  • Lee, Sang Myeong (SM Bio Co.) ;
  • Lee, Chae Min (Korea Forest Research Institute) ;
  • Lee, Chang Joon (Gyeongsangbukdo Forest and Environment Research Institute) ;
  • Kim, Dong Soo (Southern Forest Research Center, Korea Forest Research Institute) ;
  • Mun, Il Sung (Korea Forest Research Institute) ;
  • Lee, Dong Woon (Department of Applied Biology, School of Environmental Ecology and Tourism, Kyungpook National University)
  • 신원식 (경북대학교 생물응용학과, 생태환경관광학부) ;
  • 정영학 (경상대학교 대학원 응용생명과학부(BK21)) ;
  • 이상명 ((주)SM바이오) ;
  • 이채민 (국립산림과학원) ;
  • 이창준 (경상북도 산림환경연구원) ;
  • 김동수 (국립산림과학원 남부산림자원연구소) ;
  • 문일성 (국립산림과학원) ;
  • 이동운 (경북대학교 생물응용학과, 생태환경관광학부)
  • Received : 2015.11.30
  • Accepted : 2015.12.14
  • Published : 2015.12.31


Pine wood nematode (PWN), Bursaphelenchus xylophilus is one of the most serious pests of pine tree. Trunk injection of some nematicides in tree is well known as an effective control method. However there are some limitating factors which hindering the efficacy of trunk injection in field or potted tree. In this study we suggested easy and useful alternative screening methods of nematicides against PWN. Reproduction of PWN was influenced by tree twig moisture (high reproduction in high moisture trig) and paraffin coating was 78.6% reduced moisture loss in tested twig. There were no reproduction different in up and down site from infection site of twig at 1 month after inoculation of PWN and also distance (5 and 10 cm) from inoculation site of PWN did not influence the reproduction of PWN. Numbers of reproduced PWN were higher with decreasing diameter of twig. Numbers of reproduced PWN were similar to P. thunbergii and P. densiflora. However reproduction was increased depending on high inoculation density and longer propagation period. When inoculation of PWN on cut twig injected with emamectin benzoate 2.15% EC and morantel tartrate 8% SL in trunk of Pinus thunbergii in the field, PWN number were significantly reduced than untreated control. We suggest this screening method for PWN control agents.


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