- Volume 9 Issue 1
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Anti-diabetic effect of purple corn extract on C57BL/KsJ db/db mice
- Huang, Bo (College of Food Science and Engineering, Liaoning Medical University) ;
- Wang, Zhiqiang (Department of Food Science and Nutrition and Center for Aging and HealthCare, Hallym University) ;
- Park, Jong Hyuk (Institute of Natural Medicine, Hallym University Medical School) ;
- Ryu, Ok Hyun (Division of Endocrinology and Metabolism, Department of Internal Medicine, Hallym University) ;
- Choi, Moon Ki (Division of Endocrinology and Metabolism, Department of Internal Medicine, Hallym University) ;
- Lee, Jae-Yong (Institute of Natural Medicine, Hallym University Medical School) ;
- Kang, Young-Hee (Department of Food Science and Nutrition and Center for Aging and HealthCare, Hallym University) ;
- Lim, Soon Sung (Institute of Natural Medicine, Hallym University Medical School)
- Received : 2013.12.27
- Accepted : 2014.10.08
- Published : 2015.02.01
BACKGROUND/OBJECTIVES: Recently, anthocyanins have been reported to have various biological activities. Furthermore, anthocyanin-rich purple corn extract (PCE) ameliorated insulin resistance and reduced diabetes-associated mesanginal fibrosis and inflammation, suggesting that it may have benefits for the prevention of diabetes and diabetes complications. In this study, we determined the anthocyanins and non-anthocyanin component of PCE by HPLC-ESI-MS and investigated its anti-diabetic activity and mechanisms using C57BL/KsJ db/db mice. MATERIALS/METHODS: The db/db mice were divided into four groups: diabetic control group (DC), 10 or 50 mg/kg PCE (PCE 10 or PCE 50), or 10 mg/kg pinitol (pinitol 10) and treated with drugs once per day for 8 weeks. During the experiment, body weight and blood glucose levels were measured every week. At the end of treatment, we measured several diabetic parameters. RESULTS: Compared to the DC group, Fasting blood glucose levels were 68% lower in PCE 50 group and 51% lower in the pinitol 10 group. Furthermore, the PCE 50 group showed 2-fold increased C-peptide and adiponectin levels and 20% decreased HbA1c levels, than in the DC group. In pancreatic islets morphology, the PCE- or pinitol-treated mice showed significant prevention of pancreatic
Supported by : National Research Foundation of Korea (NRF)
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