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Inhibitory effect of Erythronium japonicum on the human breast cancer cell metastasis

  • You, Mi-Kyoung (Department of Food & Nutrition/Research Institute of Human Ecology, Mokpo National University) ;
  • Kim, Min-Sook (Nature Pure Korea Inc.) ;
  • Rhyu, Jin (Department of Food & Nutrition/Research Institute of Human Ecology, Mokpo National University) ;
  • Bang, Mi-Ae (Jeonnam Biofood Technology Center) ;
  • Kim, Hyeon-A (Department of Food & Nutrition/Research Institute of Human Ecology, Mokpo National University)
  • Received : 2014.07.25
  • Accepted : 2014.09.17
  • Published : 2015.02.01

Abstract

BACKGROUND/OBJECTIVES: In this study, the inhibitory effect of Erythronium japonicum extracts on the metastasis of MDA-MB-231 human breast cancer cell line was determined. MATERIALS/METHODS: Cells were cultured with DMSO or with 50, 75, 100 or $250{\mu}g/ml$ of Erythronium japonicum methanol or ethanol extract. RESULTS: Both methanol and ethanol extracts significantly inhibited the growth and induced apoptosis of MDA-MB-231 cells in a dose-dependent manner. Erythronium japonicum extracts inhibited the adhesion of MDA-MB-231 cells. The invasion of breast cancer cells was suppressed by Erythronium japonicum extracts in a dose-dependent manner. The motility and MMP-2 and MMP-9 activities were also inhibited by both methanol and ethanol extracts. CONCLUSIONS: Our results collectively indicate that Erythronium japonicum extracts inhibit the growth, adhesion, migration and invasion as well as induce the apoptosis of human breast cancer cells. Clinical application of Erythronium japonicum as a potent chemopreventive agent may be helpful in limiting breast cancer invasion and metastasis.

Acknowledgement

Supported by : Ministry of Agriculture, Food and Rural Affairs

References

  1. Yokota J. Tumor progression and metastasis. Carcinogenesis 2000;21:497-503. https://doi.org/10.1093/carcin/21.3.497
  2. Fidler IJ. The pathogenesis of cancer metastasis: the 'seed and soil' hypothesis revisited. Nat Rev Cancer 2003;3:453-8. https://doi.org/10.1038/nrc1098
  3. Evan GI, Vousden KH. Proliferation, cell cycle and apoptosis in cancer. Nature 2001;411:342-8. https://doi.org/10.1038/35077213
  4. Igney FH, Krammer PH. Death and anti-death: tumour resistance to apoptosis. Nat Rev Cancer 2002;2:277-88. https://doi.org/10.1038/nrc776
  5. Song G, Ohashi T, Sakamoto N, Sato M. Adhesive force of human hepatoma HepG2 cells to endothelial cells and expression of E-selectin. Mol Cell Biomech 2006;3:61-8.
  6. Weber GF. Molecular mechanisms of metastasis. Cancer Lett 2008;270:181-90. https://doi.org/10.1016/j.canlet.2008.04.030
  7. Festuccia C, Guerra F, D'Ascenzo S, Giunciuglio D, Albini A, Bologna M. In vitro regulation of pericellular proteolysis in prostatic tumor cells treated with bombesin. Int J Cancer 1998;75:418-31. https://doi.org/10.1002/(SICI)1097-0215(19980130)75:3<418::AID-IJC16>3.0.CO;2-4
  8. Festuccia C, Bologna M, Gravina GL, Guerra F, Angelucci A, Villanova I, Millimaggi D, Teti A. Osteoblast conditioned media contain TGF-beta1 and modulate the migration of prostate tumor cells and their interactions with extracellular matrix components. Int J Cancer 1999;81:395-403. https://doi.org/10.1002/(SICI)1097-0215(19990505)81:3<395::AID-IJC13>3.0.CO;2-V
  9. Kondo T, Okubo N, Miura T, Honda K, Ishikawa Y. Ecophysiology of seed germination in Erythronium japonicum (Liliaceae) with underdeveloped embryos. Am J Bot 2002;89:1779-84. https://doi.org/10.3732/ajb.89.11.1779
  10. Shin YJ, Jung DY, Ha HK, Park SW. Anticancer effect of Erythronium japonicum extract on ICR mouse and L1210 cells with alteration of antioxidant enzyme activities. Korean J Food Sci Technol 2004;36:968-73.
  11. Ritsema T, Smeekens S. Fructans: beneficial for plants and humans. Curr Opin Plant Biol 2003;6:223-30. https://doi.org/10.1016/S1369-5266(03)00034-7
  12. Virtanen SS, Vaananen HK, Harkonen PL, Lakkakorpi PT. Alendronate inhibits invasion of PC-3 prostate cancer cells by affecting the mevalonate pathway. Cancer Res 2002;62:2708-14.
  13. Chen PN, Hsieh YS, Chiang CL, Chiou HL, Yang SF, Chu SC. Silibinin inhibits invasion of oral cancer cells by suppressing the MAPK pathway. J Dent Res 2006;85:220-5. https://doi.org/10.1177/154405910608500303
  14. Goodman SL, Vollmers HP, Birchmeier W. Control of cell locomotion: perturbation with an antibody directed against specific glycoproteins. Cell 1985;41:1029-38. https://doi.org/10.1016/S0092-8674(85)80083-0
  15. Albini A, D'Agostini F, Giunciuglio D, Paglieri I, Balansky R, De Flora S. Inhibition of invasion, gelatinase activity, tumor take and metastasis of malignant cells by N-acetylcysteine. Int J Cancer 1995;61:121-9. https://doi.org/10.1002/ijc.2910610121
  16. Lee MS, Lim SC, Park HJ. The anthocyanin contents of the petals of Erythronium japonicum collected from several stocks. Korean J Pharmacogn 1993;24:251-4.
  17. Lee MS, Lim SC, Park HJ. Phthalate ester and flavonoids isolated from leaves of Erythronium japonicum. Korean J Med Crop Sci 1994;2:67-72.
  18. Chang WC, Hsieh CH, Hsiao MW, Lin WC, Hung YC, Ye JC. Caffeic acid induces apoptosis in human cervical cancer cells through the mitochondrial pathway. Taiwan J Obstet Gynecol 2010;49:419-24. https://doi.org/10.1016/S1028-4559(10)60092-7
  19. Hwang HJ, Park HJ, Chung HJ, Min HY, Park EJ, Hong JY, Lee SK. Inhibitory effects of caffeic acid phenethyl ester on cancer cell metastasis mediated by the down-regulation of matrix metalloproteinase expression in human HT1080 fibrosarcoma cells. J Nutr Biochem 2006;17:356-62. https://doi.org/10.1016/j.jnutbio.2005.08.009
  20. Sanderson JT, Clabault H, Patton C, Lassalle-Claux G, Jean-Francois J, Pare AF, Hebert MJ, Surette ME, Touaibia M. Antiproliferative, antiandrogenic and cytotoxic effects of novel caffeic acid derivatives in LNCaP human androgen-dependent prostate cancer cells. Bioorg Med Chem 2013;21:7182-93. https://doi.org/10.1016/j.bmc.2013.08.057
  21. Yamanaka D, Tajima K, Adachi Y, Ishibashi KI, Miura NN, Motoi M, Ohno N. Effect of polymeric caffeic acid on antitumour activity and natural killer cell activity in mice. J Funct Foods 2014;6:513-22. https://doi.org/10.1016/j.jff.2013.11.018
  22. Hazan RB, Phillips GR, Qiao RF, Norton L, Aaronson SA. Exogenous expression of N-cadherin in breast cancer cells induces cell migration, invasion, and metastasis. J Cell Biol 2000;148:779-90. https://doi.org/10.1083/jcb.148.4.779
  23. Zheng DQ, Woodard AS, Fornaro M, Tallini G, Languino LR. Prostatic carcinoma cell migration via alpha(v)beta3 integrin is modulated by a focal adhesion kinase pathway. Cancer Res 1999;59:1655-64.
  24. Hwang SL, Kim HN, Jung HH, Kim JE, Choi DK, Hur JM, Lee JY, Song H, Song KS, Huh TL. Beneficial effects of $\beta$-sitosterol on glucose and lipid metabolism in L6 myotube cells are mediated by AMP-activated protein kinase. Biochem Biophys Res Commun 2008;377:1253-8. https://doi.org/10.1016/j.bbrc.2008.10.136
  25. Kawasaki G, Yanamoto S, Yoshitomi I, Yamada S, Mizuno A. Overexpression of metastasis-associated MTA1 in oral squamous cell carcinomas: correlation with metastasis and invasion. Int J Oral Maxillofac Surg 2008;37:1039-46. https://doi.org/10.1016/j.ijom.2008.05.020
  26. Azios NG, Dharmawardhane SF. Resveratrol and estradiol exert disparate effects on cell migration, cell surface actin structures, and focal adhesion assembly in MDA-MB-231 human breast cancer cells. Neoplasia 2005;7:128-40. https://doi.org/10.1593/neo.04346
  27. Johansson N, Ahonen M, Kahari VM. Matrix metalloproteinases in tumor invasion. Cell Mol Life Sci 2000;57:5-15. https://doi.org/10.1007/s000180050495
  28. Radisky ES, Radisky DC. Matrix metalloproteinase-induced epithelialmesenchymal transition in breast cancer. J Mammary Gland Biol Neoplasia 2010;15:201-12. https://doi.org/10.1007/s10911-010-9177-x