- Volume 15 Issue 24
DOI QR Code
Candida non albicans with a High Amphotericin B Resistance Pattern Causing Candidemia among Cancer Patients
- Kalantar, Enayatollah (Department of Microbiology and Immunology, School of Medicine, Alborz University of Medical Sciences) ;
- Assadi, Mojan (Department of Oncology, School of Medicine, Alborz University of Medical Sciences) ;
- Pormazaheri, Helen (Department of Microbiology and Immunology, School of Medicine, Alborz University of Medical Sciences) ;
- Hatami, Shiva (Department of Microbiology and Immunology, School of Medicine, Alborz University of Medical Sciences) ;
- Barari, Maryam Agha (Department of Nursing, School of Nursery and Midwifery, Alborz University of Medical Sciences) ;
- Asgari, Esfandiar (Educational Supervisor Cancer Institute, Imam Khomeini Hospital, Tehran University of Medical Sciences) ;
- Mahmoudi, Elaheh (Department of Pathobiology, School of Medicine, Alborz University of Medical Sciences) ;
- Kabir, Kourosh (Department of Social Medicine, School of Medicine, Alborz University of Medical Sciences) ;
- Marashi, Seyed Mahmoud Amin (Department of Microbiology and Immunology, School of Medicine, Alborz University of Medical Sciences)
- 발행 : 2015.01.22
Background: Many scientists have reported Candida species to be of great concern because of the high frequency that they colonize and infect human hosts, particularly cancer patients. Moreover, in the last decades Candida species have developed resistance to many antifungal agents. Based on this, we aimed to identify and determine the prevalence of Candida spp from blood culture bottles among cancer patients and their antifungal resistance pattern. Materials and Methods: From the blood culture bottles isolation and identification of the Candida spp were performed by conventional microbiological techniques. The in vitro antibiotic resistance pattern of the isolates was determined by CLSI guidelines. Genomic DNA was isolated and amplified. Each gene was separated by agar gel electrophoresis. Results: Identification of Candida spp was based on the presence of yeast cells in direct examination, culture and DNA extraction. Of the 68 blood samples collected during the study period (April 2013 to October 2013), five (7.35%) were positive for the presence of Candida spp, 2 (40%) of which were identified as Candida albicans and 3 (60%) were Candida non-albicans. Conclusions: High resistance to amphotricin B was observed among all the Candida non-albicans isolates. Regular investigations into antifungal resistance will help us to get an updated knowledge about their antibiotic resistance pattern which may help the physician in selecting the antibiotics for empirical therapy.
- Badiee P, Alborzi A, Shakiba E, et al (2011). Susceptibility of Candida species isolated from immunocompromised patients to antifungal agents. EMHJ, 17, 425-30.
- Badiee P, Alborzi A (2011). Susceptibility of clinical Candida species isolates to antifungal agents by E-test, Southern Iran: A five year study. Iran J Microbiol, 3, 183-8.
- Badiee P, Alborzi A, Shakiba E, et al (2009). Molecular identification and in-vitro susceptibility of Candida albicans and Candida dubliniensis isolated from Immunocompromised patients. Iranian Red Cres Med J, 11, 391-7.
- Blumberg HM, Jarvis WR, Soucie JM (2001). Risk factors for Candidal bloodstream infections in surgical intensive care unit patients: the NEMIS prospective multicenter study. The National Epidemiology of Mycosis Survey. Clin Infect Dis, 33, 177-86. https://doi.org/10.1086/321811
- Clinical and Laboratory Standards Institute. Performance standards for antimicrobial. Susceptibility testing, 16thinformational supplement (2010). M100-S16. Clinical and Laboratory Standards. Institute Wayne, USA.
- Colomboa AL, Guimaresb T, Camargoa LFA, et al (2013). Brazilian guidelines for the management of candidiasis - a joint meeting report of three medical societies. Braz J Infect Dis, 17, 283-312. https://doi.org/10.1016/j.bjid.2013.02.001
- Edmond MB, Wallace SE, McClish DK, et al (1999). Nosocomial bloodstream infections in United States hospitals: a three-year analysis. Clin Infect Dis, 29, 239-44. https://doi.org/10.1086/520192
- Fridkin SK (2005). Candidemia is costly - plain and simple. Clin Infect Dis, 41, 1240-1. https://doi.org/10.1086/496935
- Hadley S, Lee WW, Ruthazer R, et al (2002). Candidemia as a cause of septic shock and multiple organ failure in non-immunocompromised patients. Crit Care Med, 30, 1808-14. https://doi.org/10.1097/00003246-200208000-00023
- Hajjeh RA, Sofair AN, Harrison LH (2004). Incidence of bloodstream infections due to Candida species and susceptibilities of isolates collected from 1998 to 2000 in a population-based active surveillance program. J Clin Microbiol, 42, 1519-27. https://doi.org/10.1128/JCM.42.4.1519-1527.2004
- Iranparast S, Assarehzadegan MA, Heike Y, et al (2014). Wilms' tumor gene (WT1) expression correlates with vascular epithelial growth factor (VEGF) in Newly Acute Leukemia Patients Undergoing Chemotherapy. Asian Pac J Cancer Prev, 15, 9217-23 https://doi.org/10.7314/APJCP.2014.15.21.9217
- Ligozzi M and Fontana R (2003). Isolation of total DNA from bacteria and yeast. African J Biotechnol, 2, 251-3. https://doi.org/10.5897/AJB2003.000-1052
- Mousavi S, Gouya M, Ramazani M, et al (2009). Cancer incidence and mortality in Iran. Ann Oncol, 20, 556-3.
- Naghavi M (2003). Mortality views in 23 provinces of Iran 2003. Tehran, Iran: ministry of health, deputy to health directory. Res Development Office, 7, 75-6.
- Nolte FS, Parkinson T, Falconer DJ, et al (1997). Isolation and characterization of fluconazole- and amphotericin B-Resistant Candida albicans from Blood of Two Patients with Leukemia. Antimicrob Agents Chemother, 14, 196-9.
- Theoklis E. Argon J, Chu J, et al (2005). The epidemiology and attributable outcomes of candidemia in adults and children hospitalized in the United States: a propensity analysis. Clin Infect Dis, 41, 1232-9. https://doi.org/10.1086/496922
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