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Ethanol but not Aqueous Extracts of Tubers of Sauromatum Giganteum(Engl.) Cusimano and Hett Inhibit Cancer Cell Proliferation

  • Gao, Shi-Yong (Institute of Materia Medica, Research Center of Life Science and Environmental Science, Harbin University of Commerce, and Key Laboratory of Cancer Prevention and Anticancer Drugs of Heilongjiang Province) ;
  • Li, Jun (Institute of Materia Medica, Research Center of Life Science and Environmental Science, Harbin University of Commerce, and Key Laboratory of Cancer Prevention and Anticancer Drugs of Heilongjiang Province) ;
  • Wang, Long (Institute of Materia Medica, Research Center of Life Science and Environmental Science, Harbin University of Commerce, and Key Laboratory of Cancer Prevention and Anticancer Drugs of Heilongjiang Province) ;
  • Sun, Qiu-Jia (Institute of Materia Medica, Research Center of Life Science and Environmental Science, Harbin University of Commerce, and Key Laboratory of Cancer Prevention and Anticancer Drugs of Heilongjiang Province) ;
  • Gong, Yun-Fei (Institute of Materia Medica, Research Center of Life Science and Environmental Science, Harbin University of Commerce, and Key Laboratory of Cancer Prevention and Anticancer Drugs of Heilongjiang Province) ;
  • Gang, Jian (Institute of Materia Medica, Research Center of Life Science and Environmental Science, Harbin University of Commerce, and Key Laboratory of Cancer Prevention and Anticancer Drugs of Heilongjiang Province) ;
  • Su, Yi-Jun (Institute of Materia Medica, Research Center of Life Science and Environmental Science, Harbin University of Commerce, and Key Laboratory of Cancer Prevention and Anticancer Drugs of Heilongjiang Province) ;
  • Ji, Yu-Bin (Institute of Materia Medica, Research Center of Life Science and Environmental Science, Harbin University of Commerce, and Key Laboratory of Cancer Prevention and Anticancer Drugs of Heilongjiang Province)
  • Published : 2015.01.22

Abstract

Background: Both alcohol and aqueous extracts of Sauromatum giganteum(Engl.) Cusimano and Hett, the dried root tuber of which is named Baifuzi in Chinese, have been used for folklore treatment of cancer in Northeast of China. However, little is known about which is most suitable to the cancer therapy. Materials and Methods: Serum pharmacology and MTT assays were adopted to detect the effects of ethanol and aqueous extracts of Sauromatum giganteum(Engl.) Cusimano and Hett, prepared by heat reflux methods, on proliferation of different cancer cells. Results: Cancer cells treated with medium supplemented with 10%, 20%, 40% serum(v/v) containing ethanol extract had a decline in viability, with inhibition rates of 7.69%, 21.8%, 41.9% in MCF-7 cells, 42.8%, 48.1%, 51.8% in SGC-7901 cells, 44.1%, 49.2%, 53.7% in SMMC-7721 cells, 6.8%, 15.2%, 39.8% in HepG2 cells, 7.57%, 16.3%, 36.2% in HeLa cells, 6.24%, 12.5%, 27.4% in A549 cells, and 7.20%, 17.5%, 31.3% in MDA-MB-231 cells, respectively. Viability in the aqueous extract groups was no different with that of controls. Conclusions: An ethanol extract of Sauromatum giganteum(Engl.) Cusimano and Hett inhibited the proliferation of SMMC-7721, SGC-7901 and MCF-7 cells, which supports the use of alcoholic but not aqueous extracts for control of sensive cancers, which might include hepatocarcinoma, gastric cancer and breast cancer.

Acknowledgement

Supported by : Harbin Innovation Science Foundation for Youths

References

  1. Cao Y, Xia QH, Meng H, Zhong AP (2008). Pharmacological effects of serum containing Chinese medicine Bushen Huayu Jiedu compound recipe in lung cancer drug-resistance cells. Chin J Integr Med, 14, 46-50. https://doi.org/10.1007/s11655-007-9004-4
  2. Chi S, Cai W, Liu P, et al (2010). Baifuzi reduces transient ischemic brain damage through an interaction with the STREX domain of BKCa channels. Cell Death Dis, 1, 13. https://doi.org/10.1038/cddis.2009.10
  3. Dang X-y, Dong L, Shi H-t, et al (2013). Effects of serum containing Chinese medicine Sanpi Pingwei (ae center dot c pound (TM)-a (13)e integral integral) formula on proliferation and apoptosis of human SGC-7901 cells. Chinese J Integrative Med, 19, 119-26. https://doi.org/10.1007/s11655-012-1208-6
  4. Gao SB, Wang CM, Chen XS, et al (2007). Cerebrosides of baifuzi, a novel potential blocker of calcium-activated chloride channels in rat pulmonary artery smooth muscle cells. Cell Biol Int, 31, 908-15. https://doi.org/10.1016/j.cellbi.2007.02.005
  5. Gao SY, Li J, Qu XY, Zhu N, Ji YB (2014). Downregulation of Cdk1 and CyclinB1 expression contributes to oridonin-induced cell cycle arrest at G2/M phase and growth inhibition in SGC-7901 gastric cancer cells. Asian Pac J Cancer Prev, 15, 6437-41. https://doi.org/10.7314/APJCP.2014.15.15.6437
  6. Jiang Y, Miao Y, Yang L, et al (2012). Effect of chinese herbal drug-containing serum for activating-blood and dispelling-toxin on ox-LDL-induced inflammatory factors' expression in endothelial cells. Chin J Integrative Med, 18, 30-3. https://doi.org/10.1007/s11655-011-0849-1
  7. Kurokawa M, Ohyama H, Hozumi T, et al (1996). Assay for antiviral activity of herbal extracts using their absorbed sera. Chem Pharmac Bull, 44, 1270-2. https://doi.org/10.1248/cpb.44.1270
  8. Li Q, Jiang C, Zu Y, et al (2011a). SFE-CO2 Extract from Typhonium giganteum Engl. Tubers, Induces Apoptosis in Human Hepatoma SMMC-7721 Cells Involvement of a ROS-Mediated Mitochondrial Pathway. Molecules, 16, 8228-42. https://doi.org/10.3390/molecules16108228
  9. Li XH, Wu MX, Ye HZ, et al (2011b). Experimental Study on the Suppression of Sodium Nitroprussiate-Induced Chondrocyte Apoptosis by Tougu Xiaotong Capsule ( (sic))-Containing Serum. Chin J Integr Med, 17, 436-43. https://doi.org/10.1007/s11655-011-0751-x
  10. Liu N, Liu JT, Ji YY, et al (2013). Effects and mechanisms of the functional parts of Dahuang Zhechong Pill containing serum on platelet-derived growth factor-stimulated proliferation of vascular smooth muscle cells. Chin J Integr Med, 19, 432-8. https://doi.org/10.1007/s11655-012-1033-y
  11. Ma YL LY, Tang LP, Ai FW (2010). study of Lignins of Rhizoma Typhonii on the expression of TRAIL and its receptors in gastric carcinoma cell line (SGC-7901). Chin J New Drugs, 19, 225-8.
  12. Mosmann T (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunological Methods, 65, 55-63. https://doi.org/10.1016/0022-1759(83)90303-4
  13. Sakthivel KM, Kannan N, Angeline A, et al (2012). anticancer activity of acacia nilotica (L.) Wild. Ex. delile subsp indica against dalton's ascitic lymphoma induced solid and ascitic tumor model. Asian Pac Cancer Prev, 13, 3989-95. https://doi.org/10.7314/APJCP.2012.13.8.3989
  14. Tang D, Zhang Z, Gao Y, et al (2009). Protective effects of serum containing Ginkgo biloba extract on glomerulosclerosis in rat mesangial cells. J Ethnopharmacol, 124, 26-33. https://doi.org/10.1016/j.jep.2009.04.017
  15. Tashino S (1988). “Serum pharmacology” and “serum pharmaceutical chemistry”: from pharmacology of Chinese traditional medicines to start a new measurement of drug concentration in blood. Therap Drug Monit Res, 5, 54-6.
  16. Wang BC, Zhu LC, Chen Q (2005). Primary study on the application of serum pharmacology in chinese traditional medicine. Colloids and Surfaces B-Biointerfaces, 43, 194-7. https://doi.org/10.1016/j.colsurfb.2005.04.013
  17. Wang N, Deng Y, Wei W, et al (2012). Serum containing Tongqiaohuoxue decoction suppresses glutamate-induced PC12 cell injury. Neural Regener Res, 7, 1125-31.
  18. Wang P-y, Liang X-c, Zhang H, et al (2013a). Effect of serum containing jinmaitong capsule on rats' schwann cell apoptosis induced by high glucose concentration. Chin J Integr Med, 19, 517-23. https://doi.org/10.1007/s11655-013-1506-7
  19. Wang YL, Xiao ZQ, Liu S, et al (2013b). Antidiabetic effects of Swertia macrosperma extracts in diabetic rats. J Ethnopharmacol, 150, 536-44. https://doi.org/10.1016/j.jep.2013.08.053
  20. Yin DK, Liu ZQ, Peng DY, et al (2013). Serum containing Tao-Hong-Si-wu decoction induces human endothelial cell VEGF Production via PI3K/Akt-eNOS Signaling. Evid Based Complement Alternat Med, 2013, 195158.
  21. Zhang H, Xing W-W, Li Y-S, et al (2008). Effects of a traditional Chinese herbal preparation on osteoblasts and osteoclasts. Maturitas, 61, 334-9. https://doi.org/10.1016/j.maturitas.2008.09.023
  22. Zhang S, Huang WB, Wu L, et al (2014). A novel suberoylanilide hydroxamic acid histone deacetylase inhibitor derivative, n25, exhibiting improved antitumor activity in both human U251 and H460 Cells. Asian Pac Cancer Prev, 15, 4331-8. https://doi.org/10.7314/APJCP.2014.15.10.4331
  23. Zhang Y-H, Liu J-T, Wen B-Y, et al (2009). Mechanisms of inhibiting proliferation of vascular smooth muscle cells by serum of rats treated with Dahuang Zhechong pill. J Ethnopharmacol, 124, 125-9. https://doi.org/10.1016/j.jep.2009.04.012

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