- Volume 38 Issue 1
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A Role for Peroxidasin PXN-1 in Aspects of C. elegans Development
- Lee, Juyeon (Department of Life Science, Gwangju Institute of Science and Technology) ;
- Bandyopadhyay, Jaya (Department of Biotechnology, West Bengal University of Technology) ;
- Lee, Jin Il (Division of Biological Science and Technology, College of Science and Technology, Yonsei University) ;
- Cho, Injeong (Department of Biology Education, College of Education, Chosun University) ;
- Park, Daeho (Department of Life Science, Gwangju Institute of Science and Technology) ;
- Cho, Jeong Hoon (Department of Biology Education, College of Education, Chosun University)
- Received : 2014.07.18
- Accepted : 2014.10.24
- Published : 2015.01.31
The Caenorhabditis elegans peroxidasins, PXN-1 and PXN-2, are extracellular peroxidases; pxn-2 is involved in muscle-epidermal attachment during embryonic morphogenesis and in specific axon guidance. Here we investigate potential roles of the other homologue of peroxidasin, pxn-1, in C. elegans. A pxn-1 deletion mutant showed high lethality under heat-stress conditions. Using a transcriptional GFP reporter, pxn-1 expression was observed in various tissues including neurons, muscles, and hypodermis. A translational fusion showed that PXN-1::GFP was secreted and localized in extracellular matrix, particularly along body wall muscles and pharyngeal muscles. Various neuronal developmental defects were observed in pxn-1 mutants and in pxn-1 over-expressing animals, including handedness, branching, breakage, tangling, and defasciculation. These results suggest that pxn-1, like other peroxidasins, plays an important role throughout development.
C. elegans;heat stress;neuronal development;peroxidasins;pxn-1
Supported by : National Research Foundation of Korea (NRF)
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