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Pathogen Associated Molecular Pattern (PAMP)-Triggered Immunity Is Compromised under C-Limited Growth

  • Park, Hyeong Cheol (Division of Applied Life Science (BK21 Plus Program) and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Lee, Shinyoung (Division of Applied Life Science (BK21 Plus Program) and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Park, Bokyung (Division of Applied Life Science (BK21 Plus Program) and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Choi, Wonkyun (Division of Applied Life Science (BK21 Plus Program) and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Kim, Chanmin (Division of Applied Life Science (BK21 Plus Program) and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Lee, Sanghun (Department of Botany and Plant Pathology, Purdue University) ;
  • Chung, Woo Sik (Division of Applied Life Science (BK21 Plus Program) and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Lee, Sang Yeol (Division of Applied Life Science (BK21 Plus Program) and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Sabir, Jamal (College of Science, King Abdulaziz University) ;
  • Bressan, Ray A. (Division of Applied Life Science (BK21 Plus Program) and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Bohnert, Hans J. (Division of Applied Life Science (BK21 Plus Program) and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Mengiste, Tesfaye (Department of Botany and Plant Pathology, Purdue University) ;
  • Yun, Dae-Jin (Division of Applied Life Science (BK21 Plus Program) and Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University)
  • Received : 2013.06.11
  • Accepted : 2014.10.14
  • Published : 2015.01.31

Abstract

In the interaction between plants and pathogens, carbon (C) resources provide energy and C skeletons to maintain, among many functions, the plant immune system. However, variations in C availability on pathogen associated molecular pattern (PAMP) triggered immunity (PTI) have not been systematically examined. Here, three types of starch mutants with enhanced susceptibility to Pseudomonas syringae pv. tomato DC3000 hrcC were examined for PTI. In a dark period-dependent manner, the mutants showed compromised induction of a PTI marker, and callose accumulation in response to the bacterial PAMP flagellin, flg22. In combination with weakened PTI responses in wild type by inhibition of the TCA cycle, the experiments determined the necessity of C-derived energy in establishing PTI. Global gene expression analyses identified flg22 responsive genes displaying C supply-dependent patterns. Nutrient recycling-related genes were regulated similarly by C-limitation and flg22, indicating re-arrangements of expression programs to redirect resources that establish or strengthen PTI. Ethylene and NAC transcription factors appear to play roles in these processes. Under C-limitation, PTI appears compromised based on suppression of genes required for continued biosynthetic capacity and defenses through flg22. Our results provide a foundation for the intuitive perception of the interplay between plant nutrition status and pathogen defense.

Keywords

Arabidopsis;carbon;defense;energy;flg22;NAC;starch;PAMP;Pseudomonas;PTI

Acknowledgement

Supported by : National Research Foundation of Korea (NRF)

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