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Bufalin Induces Mitochondrial Pathway-Mediated Apoptosis in Lung Adenocarcinoma Cells

  • Ding, Da-Wei (The First Clinical Medical College, Nanjing University of Chinese Medicine) ;
  • Zhang, Yong-Hong (The First Clinical Medical College, Nanjing University of Chinese Medicine) ;
  • Huang, Xin-En (Department of Medical Oncotherapy, Jiangsu Cancer Hospital) ;
  • An, Qing (Department of Intergrated Chinese and Western Medicine, Jiangsu Cancer Hospital) ;
  • Zhang, Xun (Department of Oncology, Jiangsu Province Hospital on Intergration of Chinese and Western Medicine)
  • Published : 2015.01.06

Abstract

Background: To evaluate the effects of bufalin in A549 human lung adenocarcinoma epithelial cells in vitro and assess the underlying mechanisms. Materials and Methods: Human A549 non-small cell lung cancer (NSCLC) cells were treated with various concentrations of bufalin. Cell proliferation was measured by CCK-8 assay, apoptotic cell percentage was calculated by flow cytometry and morphological change was observed by inverted phase contrast microscopy/transmission electron microscopy. In addition, the membrane potential of mitochondria was detected by JC-1 fluorescence microscopy assay, and the related protein expression of cytochrome C and caspase-3 was analyzed by Western blotting. Results: Bufalin could inhibit the proliferation of A549 cells via induction of apoptosis, with the evidence of characteristic morphological changes in the nucleus and mitochondria. Furthermore, bufalin decreased the mitochondrial membrane potential with up-regulation of cytochrome C in the cytosol, and activation of caspase-3. Conclusions: Bufalin inhibits the proliferation of A549 cells and triggers mitochondria-dependent apoptosis, pointing to therapeutic application for NSCLC.

Keywords

Bufalin-lung cancer;A549 lung cancer cells;apoptosis;mitochondrial pathway

Acknowledgement

Supported by : Graduate of Jiangsu Province

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