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Solanum Nigrum Polysaccharide (SNL) Extract Effects in Transplanted Tumor-bearing Mice - Erythrocyte Membrane Fluidity and Blocking of Functions

  • Yuan, Hong-Liang (Harbin Commercial University Life Science and Environmental Science Research Center, Harbin University of Commerce) ;
  • Liu, Xiao-Lei (College of Pharmacy, Harbin University of Commerce) ;
  • Liu, Ying-Jie (Harbin Commercial University Life Science and Environmental Science Research Center, Harbin University of Commerce)
  • Published : 2015.01.06

Abstract

Background: Solanum nigrum L. has been used in traditional Chinese medicine because of its diuretic and antipyretic effects. The present research concerned effects of crude polysaccharides isolated from Solanum nigrum L. on erythrocyte membranes of tumor-bearing $S_{180}$ and $H_{22}$ in mice. Materials and Methods: Fluorescence-labeled red blood cell membranes were used with DPH fluorescence spectrophotometry to examine erythrocyte membrane fluidity, and colorimetry to determine degree of erythrocyte surface membrane blocking. Extent of reaction by tumor-bearing mice with the enzyme erythrocyte membrane bubble shadow detection of red cell membrane variation in the degree of closure before and after administration. Results: Solanum nigrum polysaccharide could significantly improve the $S_{180}$ and $H_{22}$ tumor-bearing mice erythrocyte membrane fluidity, compared with the control group, the difference was significant (p<0.01), SNL can significantly improve the red blood cell membrane and then $S_{180}$ tumor-bearing mice sealing ability, compared with the negative control group, the difference was significant(p<0.05, p<0.01). $H_{22}$ tumor-bearing mice can increase red cell membrane and then sealing ability, the difference was significant (p<0.05). Solanum nigrum polysaccharide degree of fluidity and blocking two transplanted tumors in mice restored the ability to raise the red cell membrane has a significant effect. Conclusions: Solanum nigrum L.-type mice transplanted tumor can affect the red blood cell membrane fluidity and re-closed, through the red cell membrane of red blood cells to enhance the immune function of the possibility of erythrocyte immunity against tumor formation garland provide experimental basis.

Keywords

Solanum nigrum polysaccharide;red cell membrane;membrane fluidity;membrane blocking degree

Acknowledgement

Supported by : Natural Science Foundation

References

  1. Bayer AS, Prasad R, Chandra J, et al (2000). In vitro resistance of Staphylococcus aureus to thrombin-induced platelet microbicidal protein is associated with alterations in cytoplasmic membrane fluidity. Infect Immun, 68, 3548-53. https://doi.org/10.1128/IAI.68.6.3548-3553.2000
  2. Cardoso SM, Proenca MT, Santos S, et al (2004). Cytochrome c oxidase is decreased in Alzheimer's disease platelets. Neurobiol Aging, 25, 105-10. https://doi.org/10.1016/S0197-4580(03)00033-2
  3. Doctor A, Spinella P (2012). Effect of processing and storage on RBC function in vivo. Semin Perinatol, 36, 248-59. https://doi.org/10.1053/j.semperi.2012.04.005
  4. Gao M, Cheung KL, Lau IP, et al (2012). Polyphyllin D induces apoptosis in human erythrocytesthrough $Ca^{2+}$ rise and membrane permeabilization. Arch Toxicol, 86, 741-52. https://doi.org/10.1007/s00204-012-0808-4
  5. Hongsheng Y, Zimin L, Xiaoyun Y, et al (2014). Low-dose radiation induces antitumor effects and erythrocyte system hormesis. Asian Pac J Cancer Prev, 14, 4121-6.
  6. Jean LD, Yun X, Huiyong Z, et al (2014), Immunopreventive effects against murine $H_{22}$ hepatocellular carcinoma in vivo by a DNA vaccine targeting a gastrin-releasing peptide. Asian Pac J Cancer Prev, 15, 9039-43. https://doi.org/10.7314/APJCP.2014.15.20.9039
  7. Lu DY, Lu TR, Wu HY (2012). Development of antimetastatic drugs by targeting tumor sialic acids. Sci Pharm, 80, 497-508. https://doi.org/10.3797/scipharm.1205-01
  8. Marcela S, Xi HZ, Yang Y, et al (2008). Protein 4.1R-dependent multiprotein complex: new insights into the structural organization of the red blood cell membrane. J Cell Biology, 105, 8026-31.
  9. Narla M, Patrick G, Gallagher (2008). Red cell membrane: past, present, and future. J Blood, 112, 3939-48. https://doi.org/10.1182/blood-2008-07-161166
  10. Narla M, Xiuli A (2012). Malaria and human red blood cells. Med Microbiol Immun, 201, 593-8. https://doi.org/10.1007/s00430-012-0272-z
  11. Qinjian X, Xinli C, Lu B, et al (2014), Anti-tumor Effects and Apoptosis Induction by realgar bioleaching solution in sarcoma-180 cells in vitro and transplanted tumors in mice in vivo. Asian Pac J Cancer Prev, 15, 2883-8. https://doi.org/10.7314/APJCP.2014.15.6.2883
  12. Riedl S, Zweytick D, Lohner K (2011). Membrane-active host defense peptides-Challenges and perspectives for the development of novel anticancer drugs. Chem Phys Lipids, 164, 766-81. https://doi.org/10.1016/j.chemphyslip.2011.09.004
  13. Supabphol A, Muangman V, Chavasiri W, et al (2009). N-acetylcysteine inhibits proliferation, adhesion, migration and invasion of human bladder cancer cells. J Med Assoc Thai, 92, 1171-7.
  14. Stevenson RA, Huang JA, Studdertm J, et al (2004). Sialic acid acts as a receptor forequine rhinitisA virusbinding and infection. J Gen Virol, 85, 2535. https://doi.org/10.1099/vir.0.80207-0
  15. Shannon W, Samira D, Aaron FC, et al (2009). Genetic and biochemical modulation of sialic acid O-acetylation on group B streptococcus: phenotypic and functional impact. Glycobiology, 19, 1204-13. https://doi.org/10.1093/glycob/cwp111
  16. Son Y, Kim J, Lim JC, et al (2003), Ripe fruit of Solanum nigrum L. inhibits cell growth and induces apoptosis in MCF-7 cells. Food Chem Toxicol, 41, 1421- 8. https://doi.org/10.1016/S0278-6915(03)00161-3
  17. Xu LB, Gao SY, Ji Yb (2009).SNL on S180 tumor erythrocyte immune function in mice. Chinese Herbal Med, 40, 211-2.
  18. Yoshinori N (2011). Adaptive regulation of membrane lipids and fluidity during thermal acclimation in Tetrahymena. P Jpn Acad B-Phys, 87, 450-62. https://doi.org/10.2183/pjab.87.450
  19. Yubin J, Na L, Xiaojun Z, et al (2014), Schedule-dependent effects of kappa-selenocarrageenan in combination with epirubicin on hepatocellular carcinoma. Asian Pac J Cancer Prev, 15, 3651-7. https://doi.org/10.7314/APJCP.2014.15.8.3651
  20. Zhao YZ, Jia J, Li YB, et al (2014). Effects of endosulfan on the immune function of erythrocytes and potential protection by testosterone propionate. J Toxicol Sci, 39, 701-10. https://doi.org/10.2131/jts.39.701

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