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Association between a p73 Gene Polymorphism and Genetic Susceptibility to Non-small Cell Lung Cancer in the South of China

  • Wang, Shuang-Shuang (Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology) ;
  • Guo, Hai-Yan (Department of Obstetrics and Gynecology, Xi'an No.4 Hospital) ;
  • Dong, Lin-Li (Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology) ;
  • Zhu, Xiang-Qian (Hunan Provincial Tumor Hospital) ;
  • Ma, Liang (Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology) ;
  • Li, Wen (Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology) ;
  • Tang, Jian-Xin (Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology)
  • Published : 2015.01.06

Abstract

Background: This study aimed to identify any association between the p73 gene G4C14-to-A4T14 polymorphism and risk of non-small cell lung cancer (NSCLC) in the south of China. Materials and Methods: We genotyped the p73 gene polymorphism of peripheral blood DNA from 168 patients with NSCLC and 195 normal controls using HRM (high resolution melting) and PCR-CTPP (polymerase chain reaction with confronting two-pair primers). Results: The results of genotyping by HRM and PCR-CTPP were consistent with direct sequencing, the p73 genotype distribution in 168 lung cancer patients being as follows: GC/GC 101 cases (60.1%), GC/AT 59 cases (35.1%), AT/AT 8 cases (4.8%). The carriers of AT/AT genotype had a significantly reduced risk of NSCLC (OR=0.370; 95%CI: 0.170-0.806; p=0.010) as compared with non-carriers. However, we found no relations between p73 genotypes and histological type (p=0.798, $x^2=0.452$), tumor stage (p=0.806, $x^2=0.806$), or lymph node metastasis (p=0.578, $x^2=1.098$). Conclusions: Our findings suggest that the p73 G4C14-to-A4T14 polymorphism may be a modifier of NSCLC susceptibility in the Chinese population.

Keywords

NSCLC;p73 gene polymorphism;HRM and PCR-CTPP

Acknowledgement

Supported by : National Natural Science Foundation of China

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