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IGF-1 from Adipose-Derived Mesenchymal Stem Cells Promotes Radioresistance of Breast Cancer Cells

  • Yang, Hui-Ying (Department of Anatomy, Nanfang Hospital, Southern Medical University) ;
  • Qu, Rong-Mei (Department of Anatomy, Nanfang Hospital, Southern Medical University) ;
  • Lin, Xiao-Shan (Department of Radiation Oncology, Nanfang Hospital, Southern Medical University) ;
  • Liu, Tong-Xin (Department of Radiation Oncology, Zhejiang Cancer Hospital) ;
  • Sun, Quan-Quan (Department of Radiation Oncology, Zhejiang Cancer Hospital) ;
  • Yang, Chun (Department of Anatomy, Nanfang Hospital, Southern Medical University) ;
  • Li, Xiao-Hong (Department of Anatomy, Zunyi Medical College) ;
  • Lu, Wei (Department of Anatomy, Zunyi Medical College) ;
  • Hu, Xiao-Fang (Department of Anatomy, Zunyi Medical College) ;
  • Dai, Jing-Xing (Department of Anatomy, Nanfang Hospital, Southern Medical University) ;
  • Yuan, Lin (Department of Anatomy, Nanfang Hospital, Southern Medical University)
  • Published : 2015.01.06

Abstract

Purpose: The aim of this study was to investigate effects of adipose-derived mesenchymal stem cells (AMSCs) on radioresistance of breast cancer cells. Materials and Methods: MTT assays were used to detect any influence of AMSC supernatants on proliferation of breast cancer cells; cell migration assays were used to determine the effect of breast cancer cells on the recruitment of AMSCs; the cell survival fraction post-irradiation was assessed by clonogenic survival assay; ${\gamma}$-H2AX foci number post-irradiation was determined via fluorescence microscopy; and expression of IGF-1R was detected by Western blotting. Results: AMSC supernatants promoted proliferation and radioresistance of breast cancer cells. Breast cancer cells could recruit AMSCs, especially after irradiation. IGF-1 derived from AMSCs might be responsible for the radioresistance of breast cancer cells. Conclusions: Our results suggest that AMSCs in the tumor microenvironment may affect the outcome of radiotherapy for breast cancer in vitro.

Keywords

Adipose-derived mesenchymal stem cells;insulin-like growth factor 1;breast cancer cell proliferation

Acknowledgement

Supported by : National Natural Science Foundation of China

References

  1. Wang WE, Yang D, Li L, et al (2013). Prolyl hydroxylase domain protein 2 silencing enhances the survival and paracrine function of transplanted adipose-derived stem cells in infarcted myocardium. Circ Res, 113, 288-300 https://doi.org/10.1161/CIRCRESAHA.113.300929
  2. Xie G, Zhan J, Tian Y, et al (2012). Mammosphere cells from high-passage MCF7 cell line show variable loss of tumorigenicity and radioresistance. Cancer Lett, 316, 53-61 https://doi.org/10.1016/j.canlet.2011.10.018
  3. Yang C, Li D, Zeng W, et al (2011). Transplanted adipose-derived stem cells delay D-galactose-induced aging in rats. Neural Regen Res, 34, 2673-80.
  4. Yerushalmi R, Gelmon KA, Leung S, et al (2012). Insulin-like growth factor receptor (IGF-1R). in breast cancer subtypes. Breast Cancer Res Treat, 132, 131-42 https://doi.org/10.1007/s10549-011-1529-8
  5. Zhao M, Sachs PC, Wang X, et al (2012). Mesenchymal stem cells in mammary adipose tissue stimulate progression of breast cancer resembling the basal-type. Cancer Biol Ther, 13, 782-92 https://doi.org/10.4161/cbt.20561
  6. Zuk PA, Zhu M, Mizuno H, et al (2001). Multilineage cells from human adipose tissue, implications for cell-based therapies. Tissue Eng, 7, 211-28 https://doi.org/10.1089/107632701300062859
  7. Martin EC, Bratton MR, Zhu Y, et al (2012). Insulin-like growth factor-1 signaling regulates miRNA expression in MCF-7 breast cancer cell line. PLoS One, 7, 49067 https://doi.org/10.1371/journal.pone.0049067
  8. Martin FT, Dwyer RM, Kelly J, et al (2010). Potential role of mesenchymal stem cells (MSCs). in the breast tumour microenvironment, stimulation of epithelial to mesenchymal transition (EMT). Breast Cancer Res Treat, 124, 317-26 https://doi.org/10.1007/s10549-010-0734-1
  9. Pearl RA, Leedham SJ, Pacifico MD (2012). The safety of autologous fat transfer in breast cancer, lessons from stem cell biology. J Plast Reconstr Aesthet Surg, 65, 283-8 https://doi.org/10.1016/j.bjps.2011.07.017
  10. Perrot P, Rousseau J, Bouffaut AL, Redini F, Cassagnau E, Deschaseaux F, Heymann MF, Heymann D, et al (2010). Safety concern between autologous fat graft, mesenchymal stem cell and osteosarcoma recurrence. PLoS One, 5, 10999 https://doi.org/10.1371/journal.pone.0010999
  11. Qu YY, Hu SL, Xu XY, et al (2013). Nimotuzumab enhances the radiosensitivity of cancer cells in vitro by inhibiting radiation-induced DNA damage repair. PLoS One, 8, 70727 https://doi.org/10.1371/journal.pone.0070727
  12. Sachdev D, Yee D (2001). The IGF system and breast cancer. Endocr Relat Cancer, 8, 197-209 https://doi.org/10.1677/erc.0.0080197
  13. Semont A, Francois S, Mouiseddine M, et al (2006). Mesenchymal stem cells increase self-renewal of small intestinal epithelium and accelerate structural recovery after radiation injury. Adv Exp Med Biol, 585, 19-30
  14. Sharma M, Satyam A, Abhishek A, et al (2012). Molecular and circulatory expression of insulin growth factors in Indian females with advanced cervical cancer. Asian Pac J Cancer Prev, 13, 6475-9 https://doi.org/10.7314/APJCP.2012.13.12.6475
  15. Sun B, Roh KH, Park JR, et al (2009). Therapeutic potential of mesenchymal stromal cells in a mouse breast cancer metastasis model. Cytotherapy, 11, 289-98. https://doi.org/10.1080/14653240902807026
  16. Tai YT, Podar K, Catley L, et al (2003). Insulin-like growth factor-1 induces adhesion and migration in human multiple myeloma cells via activation of beta1-integrin and phosphatidylinositol 3'-kinase/AKT signaling. Cancer Res, 63, 5850-8
  17. Taunk NK, Goyal S, Moran MS, et al (2010). Prognostic significance of IGF-1R expression in patients treated with breast-conserving surgery and radiation therapy. Radiother Oncol, 96, 204-8 https://doi.org/10.1016/j.radonc.2010.03.009
  18. Turner BC, Haffty BG, Narayanan L, et al (1997). Insulin-like growth factor-I receptor overexpression mediates cellular radioresistance and local breast cancer recurrence after lumpectomy and radiation. Cancer Res, 57, 3079-83
  19. Ullrich A, Gray A, Tam AW, et al (1986). Insulin-like growth factor I receptor primary structure, comparison with insulin receptor suggests structural determinants that define functional specificity. Embo J, 5, 2503-12
  20. Valenciano A, Henriquez-Hernandez LA, Moreno M, Lloret M, Lara PC (2012). Role of IGF-1 receptor in radiation response. Transl Oncol, 5, 1-9 https://doi.org/10.1593/tlo.11265
  21. Vincent AM, Feldman EL (2002). Control of cell survival by IGF signaling pathways. Growth Horm Igf Res, 12, 193-7 https://doi.org/10.1016/S1096-6374(02)00017-5
  22. Begg AC, Stewart FA, Vens C (2011). Strategies to improve radiotherapy with targeted drugs. Nat Rev Cancer, 11, 239-53 https://doi.org/10.1038/nrc3007
  23. Chaput B, Foucras L, Le Guellec S, Grolleau JL, Garrido I (2013). Recurrence of an invasive ductal breast carcinoma 4 months after autologous fat grafting. Plast Reconstr Surg, 131, 123-4 https://doi.org/10.1097/01.prs.0000430109.42201.e6
  24. Eterno V, Zambelli A, Pavesi L, et al (2013). Adipose-derived Mesenchymal Stem Cells (ASCs). may favour breast cancer recurrence via HGF/c-Met signaling. Oncotarget
  25. Gee JM, Nicholson RI (2003). Expanding the therapeutic repertoire of epidermal growth factor receptor blockade, radiosensitization. Breast Cancer Res, 5, 126-9 https://doi.org/10.1186/bcr584
  26. Gooch JL, Van Den Berg CL, Yee D (1999). Insulin-like growth factor (IGF).-I rescues breast cancer cells from chemotherapy-induced cell death--proliferative and antiapoptotic effects. Breast Cancer Res Treat, 56, 1-10 https://doi.org/10.1023/A:1006208721167
  27. Green LM, Reade JL, Ware CF (1984). Rapid colorimetric assay for cell viability, application to the quantitation of cytotoxic and growth inhibitory lymphokines. J Immunol Methods 70, 257-68 https://doi.org/10.1016/0022-1759(84)90190-X
  28. Jemal A, Siegel R, Ward E, Hao Y, Xu J, Murray T, Thun MJ (2008). Cancer statistics, 2008. CA Cancer J Clin, 58, 71-96 https://doi.org/10.3322/CA.2007.0010
  29. Adams TE, Epa VC, Garrett TP, Ward CW (2000). Structure and function of the type 1 insulin-like growth factor receptor. Cell Mol Life Sci, 57, 1050-93 https://doi.org/10.1007/PL00000744
  30. Bartucci M, Morelli C, Mauro L, Ando S, Surmacz E (2001). Differential insulin-like growth factor I receptor signaling and function in estrogen receptor (ER).-positive MCF-7 and ER-negative MDA-MB-231 breast cancer cells. Cancer Res, 61, 6747-54
  31. Karnoub AE, Dash AB, Vo AP, et al (2007). Mesenchymal stem cells within tumour stroma promote breast cancer metastasis. Nature, 449, 557-63 https://doi.org/10.1038/nature06188
  32. Klopp AH, Lacerda L, Gupta A, et al (2010). Mesenchymal stem cells promote mammosphere formation and decrease E-cadherin in normal and malignant breast cells. PLoS One, 5, 12180 https://doi.org/10.1371/journal.pone.0012180
  33. Le Blanc K, Ringden O (2007). Immunomodulation by mesenchymal stem cells and clinical experience. J Intern Med, 262, 509-25 https://doi.org/10.1111/j.1365-2796.2007.01844.x
  34. Li P, Veldwijk MR, Zhang Q, et al (2013). Co-inhibition of epidermal growth factor receptor and insulin-like growth factor receptor 1 enhances radiosensitivity in human breast cancer cells. BMC Cancer, 13, 297 https://doi.org/10.1186/1471-2407-13-297
  35. Liu S, Ginestier C, Ou SJ, et al (2011). Breast cancer stem cells are regulated by mesenchymal stem cells through cytokine networks. Cancer Res, 71, 614-24 https://doi.org/10.1158/0008-5472.CAN-10-0538
  36. Liu T, Li Q, Sun Q, et al (2014). MET inhibitor PHA-665752 suppresses the hepatocyte growth factor-induced cell proliferation and radioresistance in nasopharyngeal carcinoma cells. Biochem Biophys Res Commun, 449, 49-54. https://doi.org/10.1016/j.bbrc.2014.04.147
  37. Livasy CA, Karaca G, Nanda R, et al (2006). Phenotypic evaluation of the basal-like subtype of invasive breast carcinoma. Mod Pathol, 19, 264-71 https://doi.org/10.1038/modpathol.3800528

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