IGF-1 from Adipose-Derived Mesenchymal Stem Cells Promotes Radioresistance of Breast Cancer Cells

  • Yang, Hui-Ying (Department of Anatomy, Nanfang Hospital, Southern Medical University) ;
  • Qu, Rong-Mei (Department of Anatomy, Nanfang Hospital, Southern Medical University) ;
  • Lin, Xiao-Shan (Department of Radiation Oncology, Nanfang Hospital, Southern Medical University) ;
  • Liu, Tong-Xin (Department of Radiation Oncology, Zhejiang Cancer Hospital) ;
  • Sun, Quan-Quan (Department of Radiation Oncology, Zhejiang Cancer Hospital) ;
  • Yang, Chun (Department of Anatomy, Nanfang Hospital, Southern Medical University) ;
  • Li, Xiao-Hong (Department of Anatomy, Zunyi Medical College) ;
  • Lu, Wei (Department of Anatomy, Zunyi Medical College) ;
  • Hu, Xiao-Fang (Department of Anatomy, Zunyi Medical College) ;
  • Dai, Jing-Xing (Department of Anatomy, Nanfang Hospital, Southern Medical University) ;
  • Yuan, Lin (Department of Anatomy, Nanfang Hospital, Southern Medical University)
  • Published : 2015.01.06


Purpose: The aim of this study was to investigate effects of adipose-derived mesenchymal stem cells (AMSCs) on radioresistance of breast cancer cells. Materials and Methods: MTT assays were used to detect any influence of AMSC supernatants on proliferation of breast cancer cells; cell migration assays were used to determine the effect of breast cancer cells on the recruitment of AMSCs; the cell survival fraction post-irradiation was assessed by clonogenic survival assay; ${\gamma}$-H2AX foci number post-irradiation was determined via fluorescence microscopy; and expression of IGF-1R was detected by Western blotting. Results: AMSC supernatants promoted proliferation and radioresistance of breast cancer cells. Breast cancer cells could recruit AMSCs, especially after irradiation. IGF-1 derived from AMSCs might be responsible for the radioresistance of breast cancer cells. Conclusions: Our results suggest that AMSCs in the tumor microenvironment may affect the outcome of radiotherapy for breast cancer in vitro.


Supported by : National Natural Science Foundation of China


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